Negative Regulation of hTERT Expression in Cervical Cancer Hela Cell Line by miR-21

Yang LIU; Jing LIAO; You-hui LU; Chun-yi SUN; Yu-shi MENG

doi:10.12259/j.issn.2095-610X.S20210307

Volume 42 Issue 3

Apr. 2021

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Yang LIU, Jing LIAO, You-hui LU, Chun-yi SUN, Yu-shi MENG. Negative Regulation of hTERT Expression in Cervical Cancer Hela Cell Line by miR-21[J]. Journal of Kunming Medical University, 2021, 42(3): 23-28. doi: 10.12259/j.issn.2095-610X.S20210307

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Yang LIU, Jing LIAO, You-hui LU, Chun-yi SUN, Yu-shi MENG. Negative Regulation of hTERT Expression in Cervical Cancer Hela Cell Line by miR-21[J]. Journal of Kunming Medical University, 2021, 42(3): 23-28. doi: 10.12259/j.issn.2095-610X.S20210307

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Negative Regulation of hTERT Expression in Cervical Cancer Hela Cell Line by miR-21

doi: 10.12259/j.issn.2095-610X.S20210307

1.
Dept. of Reproductive Medicine
2.
Dept. of Gynecology，The Second Affiliated Hospital of Kunming Medical University，Yunnan Kunming 650101，China

Received Date: 2020-12-26
Publish Date: 2021-04-09

Abstract

Abstract

Objective To observe the effect of miR-21 on hTERT expression in human cervical cancer Hela cell line, explore the regulatory effect of miR-21 on hTERT. Methods RT-PCR was used to detect the expressions of miR-21 and hTERT mRNA in human cervical cancer Hela cell line. West Blot was used to detect the expression of hTERT protein; miR-21 mimics, miR-21 inhibitor, mimics control and inhibitor control were designed and synthesized, then they were transfected into human cervical cancer Hela cell line after parceled by liposome 2000, blank liposome 2000 was designed as control group. The changes of expressions of hTERT mRNA and protein at 24 hours after transfection were detected. Results miR-21 and hTERT were positive in human cervical cancer Hela cell line; the relative expression levels of hTERT mRNA in mimics group, mimics control group, inhibitor group, inhibitor control group and liposome group at 24 hours after transfection were （0.51±0.33）, （0.69±0.19）, （1.33±0.39）, （0.83±0.26） and （0.58±0.16）, respectively; the relative expression levels of hTERT protein were （0.41±0.18）, （0.62±0.18）, （1.45±0.60）, （0.83±0.15） and （0.82±0.30）, respectively. The relative expression levels of hTERT mRNA and protein in mimics group were statistically significantly lower than those in mimics control group and liposome group （P < 0.05）. The relative expression levels of hTERT mRNA and protein in inhibitor group were statistically significantly higher than those in inhibitor control group and liposome group （P < 0.05）. Conclusions miR-21 and hTERT genes are highly expressed in HeLa cell lines. MiR-21 can regulate hTERT mRNA and protein expression levels, thus negatively regulating hTERT.
- miR-21,
- hTERT,
- Cervical carcinoma,
- Hela,
- Negative regulation

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