Effect and Mechanism of Puerarin on Experimental Autoimmune Encephalomyelitis in Mice

Guo-yi LIU; Qing-qing ZHAO; Yan WU; Shan-shan LI; Lian-mei ZHONG; Jia GENG

doi:10.12259/j.issn.2095-610X.S20210401

Volume 42 Issue 4

Apr. 2021

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Guo-yi LIU, Qing-qing ZHAO, Yan WU, Shan-shan LI, Lian-mei ZHONG, Jia GENG. Effect and Mechanism of Puerarin on Experimental Autoimmune Encephalomyelitis in Mice[J]. Journal of Kunming Medical University, 2021, 42(4): 1-7. doi: 10.12259/j.issn.2095-610X.S20210401

Citation:

Guo-yi LIU, Qing-qing ZHAO, Yan WU, Shan-shan LI, Lian-mei ZHONG, Jia GENG. Effect and Mechanism of Puerarin on Experimental Autoimmune Encephalomyelitis in Mice[J]. Journal of Kunming Medical University, 2021, 42(4): 1-7. doi: 10.12259/j.issn.2095-610X.S20210401

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Effect and Mechanism of Puerarin on Experimental Autoimmune Encephalomyelitis in Mice

doi: 10.12259/j.issn.2095-610X.S20210401

1.
Dept. of Neurology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
2.
Dept of Human Anatomy，Histology and Embryology，School of Basic Medicine，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2020-12-26
Publish Date: 2021-04-01

Abstract

Abstract

Objective To explore the effect and mechanism of Puerarin on experimental autoimmune encephalomyelitis （EAE） in mice. Methods C57BL / 6 mice were randomly divided into control group, EAE group and purearin treated EAE group. From the first day of EAE induction, puerarin group was gavaged with Puerarin （80 mg/kg）, and the other two groups were given the same volume of normal saline once a day. The neurological function score and weight changes were recorded every day; the demyelination of spinal cord was detected by LFB myelination staining; the levels of IL-6, IL-10, IL-17, IL-22 and TGF-β in serum were detected by ELISA; the protein expression of NF-κB, IκBα, IL-17, Foxp3 in spinal cord tissue were detected by Western blot. Results Compared with EAE group, the neurological function score and average score of purearin group were significantly decreased （P < 0.01）, while the body weight was significantly increased （P < 0.01）. The demyelinating area （P < 0.01） and pathological score （P < 0.05） of EAE mice were significantly decreased after puerarin treatment. The levels of proinflammatory cytokines IL-6, IL-17 and IL-22 were significantly decreased in puerarin group （P < 0.01）, while IL-10 and TGF-β were significantly increased （P < 0.05）. After puerarin treatment, the expressions of NF-κB and IL-17 significantly decreased （P < 0.05）, and the expressions of IκBα and Foxp3 significantly increased （P < 0.01）. Conclusion Puerarin can alleviate the symptoms of EAE mice and reduce demyelination. The mechanism of action is to inhibit NF-κB pathway, to balance of IL-17/Tregs, and to regulate of immune function.
- Experimental autoimmune encephalomyelitis,
- Puerarin,
- Th17,
- Treg,
- NF-κB pathway

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References(17)

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