Effect and Mechanism of Puerarin on Experimental Autoimmune Encephalomyelitis in Mice

Guo-yi LIU; Qing-qing ZHAO; Yan WU; Shan-shan LI; Lian-mei ZHONG; Jia GENG

doi:10.12259/j.issn.2095-610X.S20210401

Volume 42 Issue 4

Apr. 2021

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2021 > 42(4): 1-7

Guo-yi LIU, Qing-qing ZHAO, Yan WU, Shan-shan LI, Lian-mei ZHONG, Jia GENG. Effect and Mechanism of Puerarin on Experimental Autoimmune Encephalomyelitis in Mice[J]. Journal of Kunming Medical University, 2021, 42(4): 1-7. doi: 10.12259/j.issn.2095-610X.S20210401

Citation:

Guo-yi LIU, Qing-qing ZHAO, Yan WU, Shan-shan LI, Lian-mei ZHONG, Jia GENG. Effect and Mechanism of Puerarin on Experimental Autoimmune Encephalomyelitis in Mice[J]. Journal of Kunming Medical University, 2021, 42(4): 1-7. doi: 10.12259/j.issn.2095-610X.S20210401

Citation:

PDF( 966 KB)

Effect and Mechanism of Puerarin on Experimental Autoimmune Encephalomyelitis in Mice

doi: 10.12259/j.issn.2095-610X.S20210401

1.
Dept. of Neurology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
2.
Dept of Human Anatomy，Histology and Embryology，School of Basic Medicine，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2020-12-26
Publish Date: 2021-04-01

Abstract

Abstract

Objective To explore the effect and mechanism of Puerarin on experimental autoimmune encephalomyelitis （EAE） in mice. Methods C57BL / 6 mice were randomly divided into control group, EAE group and purearin treated EAE group. From the first day of EAE induction, puerarin group was gavaged with Puerarin （80 mg/kg）, and the other two groups were given the same volume of normal saline once a day. The neurological function score and weight changes were recorded every day; the demyelination of spinal cord was detected by LFB myelination staining; the levels of IL-6, IL-10, IL-17, IL-22 and TGF-β in serum were detected by ELISA; the protein expression of NF-κB, IκBα, IL-17, Foxp3 in spinal cord tissue were detected by Western blot. Results Compared with EAE group, the neurological function score and average score of purearin group were significantly decreased （P < 0.01）, while the body weight was significantly increased （P < 0.01）. The demyelinating area （P < 0.01） and pathological score （P < 0.05） of EAE mice were significantly decreased after puerarin treatment. The levels of proinflammatory cytokines IL-6, IL-17 and IL-22 were significantly decreased in puerarin group （P < 0.01）, while IL-10 and TGF-β were significantly increased （P < 0.05）. After puerarin treatment, the expressions of NF-κB and IL-17 significantly decreased （P < 0.05）, and the expressions of IκBα and Foxp3 significantly increased （P < 0.01）. Conclusion Puerarin can alleviate the symptoms of EAE mice and reduce demyelination. The mechanism of action is to inhibit NF-κB pathway, to balance of IL-17/Tregs, and to regulate of immune function.
- Experimental autoimmune encephalomyelitis,
- Puerarin,
- Th17,
- Treg,
- NF-κB pathway

FullText(HTML)

References(17)

References

[1]	Denrou C A,Fugger L,FriseR M A. Immunopathology of multiple sclerosis[J]. Nature reviews. Immunology,2015,15(9):545-558. doi: 10.1038/nri3871
[2]	Correale J,Gaitan M I,Ysrraelit M C,et al. Progressive multiple sclerosis:from pathogenic mechanisms to treatment[J]. Brain:A journal of neurology,2017,140(3):527-546.
[3]	Cai Y,Shen H,Qin C,et al. The Spatio-Temporal Expression Profiles of CD4+T Cell Differentiation and Function-Related Genes During EAE Pathogenesis[J]. Inflammation,2017,40(1):195-204.
[4]	Luckel C,Picard F,Raifer H,et al. IL-17 CD8 T cell suppression by dimethyl fumarate associates with clinical response in multiple sclerosis[J]. Nature communications,2019,10(1):5722. doi: 10.1038/s41467-019-13731-z
[5]	Eggert M,Goertsches R,Seeck U,et al. Changes in the activation level of NF-kappa B in lymphocytes of MS patients during glucocorticoid pulse therapy[J]. Journal of the neurological sciences,2008,264(1-2):145-150. doi: 10.1016/j.jns.2007.08.026
[6]	Kipp M,Nyamoya S,Hochstrasser T,et al. Multiple sclerosis animal models:a clinical and histopathological perspective[J]. Brain Pathol,2017,27(2):123-137.
[7]	Chao C C,Gutierrez-Vazquez C,Rothhammer V,et al. Metabolic Control of Astrocyte Pathogenic Activity via cPLA2-MAVS[J]. Cell,2019,179(7):1483-1498 e1422. doi: 10.1016/j.cell.2019.11.016
[8]	Correa J O,Aarestrup B J,Aarestrup F M. Effect of thalidomide and pentoxifylline on experimental autoimmune encephalomyelitis (EAE)[J]. Exp Neurol,2010,226(1):15-23.
[9]	Toyama M,Kudo D,Aoyagi T,et al. Attenuated accumulation of regulatory T cells and reduced production of interleukin 10 lead to the exacerbation of tissue injury in a mouse model of acute respiratory distress syndrome[J]. Microbiol Immunol,2018,62(2):111-123. doi: 10.1111/1348-0421.12564
[10]	Wang N,Liang S,Jin J,et al. CD226 attenuates Treg suppressive capacity via CTLA-4 and TIGIT during EAE[J]. Immunol Res,2019,67(6):486-496. doi: 10.1007/s12026-019-09112-9
[11]	Hou H,Cao R,Quan M,et al. Rapamycin and fingolimod modulate Treg/Th17 cells in experimental autoimmune encephalomyelitis by regulating the Akt-mTOR and MAPK/ERK pathways[J]. J Neuroimmunol,2018,324(1):26-34. doi: 10.1016/j.jneuroim.2018.08.012
[12]	Lin X,Liu Y,Ma L,et al. Amelioration of experimental autoimmune encephalomyelitis by Rhodiola rosea,a natural adaptogen[J]. Biomed Pharmacother,2020,125(1):109960. doi: 10.1016/j.biopha.2020.109960
[13]	Li J,Chen Y,Chen Z,et al. Therapeutic effects of human adipose tissue-derived stem cell (hADSC) transplantation on experimental autoimmune encephalomyelitis (EAE) mice[J]. Scientific reports,2017,7(2):42695.
[14]	Zhan T,Wang X,Ouyang Z,et al. Rotating magnetic field ameliorates experimental autoimmune encephalomyelitis by promoting T cell peripheral accumulation and regulating the balance of Treg and Th1/Th17[J]. Aging,2020,12(7):6225-6239. doi: 10.18632/aging.103018
[15]	Mc Guire C,Volckaert T,Wolke U,et al. Oligodendrocyte-specific FADD deletion protects mice from autoimmune-mediated demyelination[J]. Journal of immunology (Baltimore,Md.:1950),2010,185(12):7646-7653. doi: 10.4049/jimmunol.1000930
[16]	Wang Z-K,Chen R-R,Li J-H,et al. Puerarin protects against myocardial ischemia/reperfusion injury by inhibiting inflammation and the NLRP3 inflammasome:The role of the SIRT1/NF-κB pathway[J]. International immunopharmacology,2020,89(Pt B):107086.
[17]	Jeon Y-D,Lee J-H,Lee Y-M,et al. Puerarin inhibits inflammation and oxidative stress in dextran sulfate sodium-induced colitis mice model[J]. Biomedecine & pharmacotherapie,2020,124(1):109847.

Relative Articles(20)

Relative Articles

[1]	Zhuohui LIU, Shiyin QIN, Hexiang ZHAO, Fengfeng JIA, Biao RUAN, Ruiqing LONG. Inhibitory Effect of Crocin on Pituitary Adenomas via IRF7/NF-κB Signaling Pathway. Journal of Kunming Medical University, 2024, 46(): 1-9.
[2]	Aheng WANG, Zhen SONG, Guanghua YANG, Haiyan HE. Regulatory Mechanisms of TLR4/NF-κB/ MUC5B in Allergic Rhinitis. Journal of Kunming Medical University, 2024, 45(11): 1-6.
[3]	Tingna ZHU, Yuanyuan CAO, Yuan ZHANG, Pengliang LIU, Yihang WANG, Yamei WU, Lihua LI, Yongna ZHAO, Shijun HONG. Effects of Different Doses of Gastrodin on TLR4/MyD88/NF-κB Inflammatory Signal Pathway in Hippocampus of Methamphetamine Dependent CPP Rats. Journal of Kunming Medical University, 2023, 44(2): 33-39. doi: 10.12259/j.issn.2095-610X.S20230226
[4]	Yidan FU, Wenting CHEN, Xiaoyang SU, Yan ZHAO, Danfeng LAN, Qiuping YANG. Role of Mertk-mediated NF-κb Pathway in Inflammatory Response of Schwann Cells. Journal of Kunming Medical University, 2023, 44(12): 20-24. doi: 10.12259/j.issn.2095-610X.S20231203
[5]	Jun HE, Xuezhong GU, Yating LIN, Fan LI. Research Progress on the Mechanism of Regulatory T Cells in Tumor Microenvironment of Diffuse Large B-cell Lymphoma. Journal of Kunming Medical University, 2023, 44(6): 150-154. doi: 10.12259/j.issn.2095-610X.S20230607
[6]	Chen WANG, Sha LI, Boxue ZHANG, Jinhua HE. Effect of VitD Supplementation on the Improvement of Autoimmune Thyroiditis in Childbearing Age Females. Journal of Kunming Medical University, 2022, 43(9): 135-140. doi: 10.12259/j.issn.2095-610X.S20220907
[7]	Kun ZHAO, Yun XIAO, Chun YANG, Zhiling YAN, Minna DONG, Bingquan XIANG, Mingyao XIAO. Protective Effect of Interleukin-4 in Lipopolysaccharide-induced Acute Lung Injury Models. Journal of Kunming Medical University, 2022, 43(8): 41-46. doi: 10.12259/j.issn.2095-610X.S20220805
[8]	Le ZHAN, Ruiyu MA, Ni WAN, Liwen MO, Huiying CHEN, Li ZHANG. Effects of Puerarin on Th17/Treg Cell Immune Homeostasis and the Expression of Related Transcription Factors in Rats with Periodontitis. Journal of Kunming Medical University, 2022, 43(11): 36-43. doi: 10.12259/j.issn.2095-610X.S20221129
[9]	Zhi-qiang LI, Hong-qiang GAO, Jing-feng LI, Jie-qi YU, Dong-dong WANG, Gang CHEN, Guang CHU. Expression of ICAM-1, MMP-9 and NF-κB in Cholangiocarcinoma and Their Relationship with Immunosuppressive Cells in Peripheral Blood. Journal of Kunming Medical University, 2021, 42(11): 93-98. doi: 10.12259/j.issn.2095-610X.S20211117
[10]	Jun-jie LI, Hai-yan JIANG, Wen-ya BAI, Si-ying HUO, Zhi-sheng SUN, Jian-lin SHAO. Effects of Silencing RND3 Expression on the Inflammatory Response and Apoptosis of Hippocampal Neurons Injured by Oxygen Glucose Deprivation/Reoxygenation. Journal of Kunming Medical University, 2021, 42(10): 14-21. doi: 10.12259/j.issn.2095-610X.S20211012
[11]	Huang Ying , Xu Kun Jing , Pu Dong , Li Xiao Fei , Chen Hai Yun , Wu Yan , Wang Li Hua , Li Wen Ming , Luo Yun , He Hua . . Journal of Kunming Medical University, 2019, 40(02): 116-122.
[12]	Long Jun Jun , Li Lan . . Journal of Kunming Medical University, 2019, 40(11): 152-155.
[13]	Fan Yi Dan , Chen Yu , Rao Chun Mei , Gao Xue Juan , Zhang Fang , Fang Shan Dan , Xiang Run Qing , Fan Yuan , Wu Yang . . Journal of Kunming Medical University, 2018, 39(11): 62-66.
[14]	Tang Wei Wei , He Yong Wen . . Journal of Kunming Medical University, 2018, 39(10): 42-45.
[15]	Tian Bo Yan , Yang Qin Ling , Zhang Jiao Qiong , Han Na , Liu Shao Hua . The Curative Effect of Auxiliary Hepatic Arterial Chemoembolization in the Treatment of Microvascular Invasion of Hepatocellular Carcinoma. Journal of Kunming Medical University, 2018, 39(08): 86-92.
[16]	Zhang Nan Yang , Pan Yu , Wu Guo Dong , Zeng Yue Qin , Chen Liang . . Journal of Kunming Medical University, 2018, 39(12): 23-27.
[17]	Ren Chao Feng . Cuture of Regulatory T Cells and the Changes of Immune Factors in COPD Rats after Venous injection of Regulatory T Cells. Journal of Kunming Medical University,
[18]	Jiang Yan . The Relationship of Regulatory T Cells and Senile Osteoporosis. Journal of Kunming Medical University,
[19]	Lu Ping . . Journal of Kunming Medical University,
[20]	. Clinical and Pathological Characteristics of 92 Patients with Autoimmune Hepatitis. Journal of Kunming Medical University,