Metabonomics of  Scopolamine Intoxication in Rats

Ming YAN; Jian-yun YU; Sheng-jie NIE; Yong-qiang QU; Shang-wen WANG; Rui WANG; Jun-jie SHU; Huan LIU; Shu-hua LI

doi:10.12259/j.issn.2095-610X.S20210403

Volume 42 Issue 4

Apr. 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(4): 13-19

Ming YAN, Jian-yun YU, Sheng-jie NIE, Yong-qiang QU, Shang-wen WANG, Rui WANG, Jun-jie SHU, Huan LIU, Shu-hua LI. Metabonomics of Scopolamine Intoxication in Rats[J]. Journal of Kunming Medical University, 2021, 42(4): 13-19. doi: 10.12259/j.issn.2095-610X.S20210403

Citation:

Ming YAN, Jian-yun YU, Sheng-jie NIE, Yong-qiang QU, Shang-wen WANG, Rui WANG, Jun-jie SHU, Huan LIU, Shu-hua LI. Metabonomics of Scopolamine Intoxication in Rats[J]. Journal of Kunming Medical University, 2021, 42(4): 13-19. doi: 10.12259/j.issn.2095-610X.S20210403

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Metabonomics of Scopolamine Intoxication in Rats

doi: 10.12259/j.issn.2095-610X.S20210403

School of Forensic Medicine，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2020-12-14
Publish Date: 2021-04-01

Abstract

Abstract

Objective To study the relationship between different metabolites, metabolic pathways and toxicity in scopolamine intoxicated rats. Methods 40 Sprague-Dawley （SD） rats were divided into control group and 3 groups with different doses of drugs. Chronic intoxication model was established after continuous administration for 30 days. By using Gas chromatography-mass spectrometry （GC/MSD）, and multivariate analysis and database retrieval, endogenous metabolites in serum and urine of different groups of rats were analyzed to screen and identify potential differential metabolites. Metabolic pathway enrichment was carried out by MetaboAnalyst software. The paraffin sections were stained with HE and observed microscopically. Results The metabolic profile of the control group and the administration group was significantly different （P < 0.05）. In the serum and urine samples of the drug administration groups, 17 different metabolites, including glutamic acid, glycine, proline, creatine and alanine, were screened out, mainly involving 9 metabolic pathways, including alanine-aspartate-glutamic acid metabolism, glycine-serine-threonine metabolism, and glutamine-glutamic acid metabolism, etc. The main pathological result was neurodegeneration. Conclusion The toxic effect of scopolamine is mainly neurotoxicity, and its mechanism of toxicity may be related to its disturbance of alanine-aspartic acid-glutamic acid metabolism, glycine-serine-threonine metabolism, glutamine-glutamic acid metabolism.
- Scopolamine,
- Rats,
- Chronic poisoning model,
- Metabonomics

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References(15)

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