Metabonomics of  Scopolamine Intoxication in Rats

Ming YAN; Jian-yun YU; Sheng-jie NIE; Yong-qiang QU; Shang-wen WANG; Rui WANG; Jun-jie SHU; Huan LIU; Shu-hua LI

doi:10.12259/j.issn.2095-610X.S20210403

Volume 42 Issue 4

Apr. 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(4): 13-19

Ming YAN, Jian-yun YU, Sheng-jie NIE, Yong-qiang QU, Shang-wen WANG, Rui WANG, Jun-jie SHU, Huan LIU, Shu-hua LI. Metabonomics of Scopolamine Intoxication in Rats[J]. Journal of Kunming Medical University, 2021, 42(4): 13-19. doi: 10.12259/j.issn.2095-610X.S20210403

Citation:

Ming YAN, Jian-yun YU, Sheng-jie NIE, Yong-qiang QU, Shang-wen WANG, Rui WANG, Jun-jie SHU, Huan LIU, Shu-hua LI. Metabonomics of Scopolamine Intoxication in Rats[J]. Journal of Kunming Medical University, 2021, 42(4): 13-19. doi: 10.12259/j.issn.2095-610X.S20210403

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Metabonomics of Scopolamine Intoxication in Rats

doi: 10.12259/j.issn.2095-610X.S20210403

School of Forensic Medicine，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2020-12-14
Publish Date: 2021-04-01

Abstract

Abstract

Objective To study the relationship between different metabolites, metabolic pathways and toxicity in scopolamine intoxicated rats. Methods 40 Sprague-Dawley （SD） rats were divided into control group and 3 groups with different doses of drugs. Chronic intoxication model was established after continuous administration for 30 days. By using Gas chromatography-mass spectrometry （GC/MSD）, and multivariate analysis and database retrieval, endogenous metabolites in serum and urine of different groups of rats were analyzed to screen and identify potential differential metabolites. Metabolic pathway enrichment was carried out by MetaboAnalyst software. The paraffin sections were stained with HE and observed microscopically. Results The metabolic profile of the control group and the administration group was significantly different （P < 0.05）. In the serum and urine samples of the drug administration groups, 17 different metabolites, including glutamic acid, glycine, proline, creatine and alanine, were screened out, mainly involving 9 metabolic pathways, including alanine-aspartate-glutamic acid metabolism, glycine-serine-threonine metabolism, and glutamine-glutamic acid metabolism, etc. The main pathological result was neurodegeneration. Conclusion The toxic effect of scopolamine is mainly neurotoxicity, and its mechanism of toxicity may be related to its disturbance of alanine-aspartic acid-glutamic acid metabolism, glycine-serine-threonine metabolism, glutamine-glutamic acid metabolism.
- Scopolamine,
- Rats,
- Chronic poisoning model,
- Metabonomics

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References(15)

References

[1]	董红锰,武军元,李毅贤,等. 急性曼陀罗中毒五例患者的诊治[J]. 中华急诊医学杂志,2020,25(5):730-731. doi: 10.3760/cma.j.issn.1671-0282.2020.05.024
[2]	翟丽梅,刘舒畅,燕宪亮,等. 家庭聚集性急性曼陀罗中毒7例临床分析[J]. 中国急救医学,2020,40(12):1205-1206.
[3]	张盛,蔡旗旗,林弘航,等. 一起社区群体性急性曼陀罗籽中毒5例救治体会[J]. 中国乡村医药,2020,27(19):30-31.
[4]	马旭霞,汪天宇,岳莹莹,等. 东莨菪碱快速抗抑郁的研究进展[J]. 中华精神科杂志,2020,53(3):258-262.
[5]	张均田, 吴冠华. 现代药理实验方法[M]. 北京: 北京协和医科大学出版社, 2010: 2173-2174.
[6]	骆利群. 神经生物学原理[M]. 北京: 高等教育出版社, 2018: 356.
[7]	彭国茳,史碧云,田俊生,等. 京尼平抗抑郁作用的~1H NMR代谢组学机制研究[J]. 药学学报,2014,40(2):209-216.
[8]	Kvamme E. Synthesis of glutamate and its regulation[J]. Prog Brain Res,1998,116(8):73-85.
[9]	Fleck M W,Henze D A,Barrionuevo G,et al. Aspartate and glutamate mediate excitatory synaptic transmission in area CA1 of the hippocampus[J]. J Neurosci,1993,13(9):3944-3955. doi: 10.1523/JNEUROSCI.13-09-03944.1993
[10]	Meldrum B S. Glutamate as a neurotransmitter in the brain:Review of physiology and pathology[J]. J Nutr,2000,130(4S Suppl):1007s-1015s.
[11]	Tang Y P,Shimizu E,Dube G R,et al. Genetic enhancement of learning and memory in mice[J]. Nature,1999,401(6748):63-69. doi: 10.1038/43432
[12]	徐向华,潘雅萍,王晓良. 东莨菪碱致记忆障碍大鼠中枢电压依赖性钾通道亚型的表达[J]. 药学学报,2002,49(4):241-244. doi: 10.3321/j.issn:0513-4870.2002.04.001
[13]	盖世英,刘伟国,马力,等. 谷氨酸介导中枢系统损伤神经毒性的分子机制概述[J]. 浙江创伤外科,2010,15(3):407-409. doi: 10.3969/j.issn.1009-7147.2010.03.097
[14]	Rajedra S,Lynch J W,Schofield P R. The glycine receptor[J]. Pharmacol Ther,1997,73(2):121-146. doi: 10.1016/S0163-7258(96)00163-5
[15]	扈启宽,陈宜张. 甘氨酸受体[J]. 生理科学进展,1993,47(3):267-268.

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