Aqueous Extract of <i>Moringa Oleifera</i><italic/> Leaves Alleviate Olanzapine-Induced Metabolic Syndrome in Mice

Xue-fang YANG; Rong XIAO; Shun-shan LIAO; De-hong CAI; Zhi-bi ZHANG; Jian-kun LIU

doi:10.12259/j.issn.2095-610X.S20210404

Volume 42 Issue 4

Apr. 2021

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Xue-fang YANG, Rong XIAO, Shun-shan LIAO, De-hong CAI, Zhi-bi ZHANG, Jian-kun LIU. Aqueous Extract of Moringa Oleifera Leaves Alleviate Olanzapine-Induced Metabolic Syndrome in Mice[J]. Journal of Kunming Medical University, 2021, 42(4): 20-27. doi: 10.12259/j.issn.2095-610X.S20210404

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Xue-fang YANG, Rong XIAO, Shun-shan LIAO, De-hong CAI, Zhi-bi ZHANG, Jian-kun LIU. Aqueous Extract of Moringa Oleifera Leaves Alleviate Olanzapine-Induced Metabolic Syndrome in Mice[J]. Journal of Kunming Medical University, 2021, 42(4): 20-27. doi: 10.12259/j.issn.2095-610X.S20210404

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Aqueous Extract of Moringa Oleifera Leaves Alleviate Olanzapine-Induced Metabolic Syndrome in Mice

doi: 10.12259/j.issn.2095-610X.S20210404

1.
Incubation Center for Scientific and Technological Achievements，Kunming Yunnan 650500
2.
First Clinical College，Kunming Medical University， Kunming Yunnan 650032
3.
School of Pharmaceutical Science，Kunming Medical University
4.
Biomedical Engineering Research Center，Kunming Medical University，Kunming Yunnan 650500
5.
Dept of Gastroenterology，No. 920 Hospital of the Joint Security Force，Kunming Yunnan 650032， China

Received Date: 2020-12-18
Publish Date: 2021-04-01

Abstract

Abstract

Objective To explore the protective effect and mechanisms of aqueous extract of Moringa oleifera leaves （EMO） on olanzapine （OLA） induced metabolic disorders in mice. Method 90 female mice were randomly divided into control group, OLA group （3 mg/kg）, positive control drug group （OLA + metformin （MET）, 3 mg/kg + 75 mg/kg）, EMO group （EMO-H/M/L. 400/200/100 mg/kg）, OLA + EMO group （OLA + EMO-H/M/L, 3 mg/kg + 400/200/100）. Each group was given intragastric administration for 14 days, and weight gain, food and water intake, fasting blood glucose, blood lipid, serum leptin, ghrelin, malondialdehyde （MDA）, serum peroxidase dismutase （SOD） activity, glutathione peroxidase （GSH-Px） activity, liver fatty acid synthase （FAS） and adipose differentiation related protein （ADRP） gene mRNA expression, liver tissue pathology damage were detected. Result Compared with control group, in OLA group food intake increased significantly （P < 0.01）, the contents of FBG, THC, TG and LDL-C, the levels of serum leptin and ghrelin increased significantly （P < 0.05 or P < 0.01）, and the expression of liver FAS and ADRP genes was significantly increased （P < 0.01）. Serum SOD and GSH-Px enzyme activities were significantly reduced （P < 0.05）, MDA content was significantly increased （P < 0.05）, and liver tissue damage was severe. Compared with the OLA group, in OLA + EMO-H / M group food and water intake significantly reduced （P < 0.05 or P < 0.01）, body weight gain significantly reduced （P < 0.05 or P < 0.01）, serum FBG and THC, TG, and LDL-C levels were significantly reduced （P < 0.05）, leptin and ghrelin levels were significantly reduced （P < 0.05）. Liver FAS and ADRP gene mRNA expression was significantly down-regulated, serum SOD and GSH-Px enzyme activities were significantly increased （P < 0.05）, MDA concentration was significantly reduced （P < 0.05）, and liver tissue damage was partially reversed. Compared the Control group, in the EMO group the above indicators were not significantly different. Conclusion EMO may partially reverse the glucose and lipid metabolism disorder induced by olanzapine. The mechanism is that the antioxidant effect protects the normal functions of tissues and organs, reduces the secretion of leptin and ghrelin, and reduces the symptoms of overeating. In addition, it can protect the cellular function of liver tissue, down-regulate the expression of genes related to fatty acid synthesis and transport, and reduce the content of blood lipid.
- Moringa oleifera leaves,
- Olanzapine,
- Metabolic syndrome,
- Antioxidant

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References(18)

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