Expression of XB130 in Hepatocellular Carcinoma and Its Effect on Cell Invasion and Migration

Song-xu QI; Cheng QIU; Rong-jun CHEN; Zhan-hui CHEN; Wan-yu ZHANG; Shi-long TANG; Fan CAO; Chun-man LI; Nan XIE

doi:10.12259/j.issn.2095-610X.S20210413

Volume 42 Issue 4

Apr. 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(4): 67-72

Song-xu QI, Cheng QIU, Rong-jun CHEN, Zhan-hui CHEN, Wan-yu ZHANG, Shi-long TANG, Fan CAO, Chun-man LI, Nan XIE. Expression of XB130 in Hepatocellular Carcinoma and Its Effect on Cell Invasion and Migration[J]. Journal of Kunming Medical University, 2021, 42(4): 67-72. doi: 10.12259/j.issn.2095-610X.S20210413

Citation:

Song-xu QI, Cheng QIU, Rong-jun CHEN, Zhan-hui CHEN, Wan-yu ZHANG, Shi-long TANG, Fan CAO, Chun-man LI, Nan XIE. Expression of XB130 in Hepatocellular Carcinoma and Its Effect on Cell Invasion and Migration[J]. Journal of Kunming Medical University, 2021, 42(4): 67-72. doi: 10.12259/j.issn.2095-610X.S20210413

Citation:

Song-xu QI, Cheng QIU, Rong-jun CHEN, Zhan-hui CHEN, Wan-yu ZHANG, Shi-long TANG, Fan CAO, Chun-man LI, Nan XIE. Expression of XB130 in Hepatocellular Carcinoma and Its Effect on Cell Invasion and Migration[J]. Journal of Kunming Medical University, 2021, 42(4): 67-72. doi: 10.12259/j.issn.2095-610X.S20210413

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Expression of XB130 in Hepatocellular Carcinoma and Its Effect on Cell Invasion and Migration

doi: 10.12259/j.issn.2095-610X.S20210413

1.
Dept. of Hepatology，Donghua Hospital of Sun Yat-sen University，Dongguan Guangdong 523000
2.
Dept. of Hepatopancreatobiliary Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2020-12-11
Publish Date: 2021-04-01

Abstract

Abstract

Objective To investigate the expression of new adaptor protein XB130 in hepatocellular carcinoma （HCC） tissues and adjacent tissues and its relationship with histopathology, and to explore its influence on the invasion and migration of hepatocellular carcinoma cells. Methods We collected and analyzed the pathological sections of stained HCC samples. The expression of XB130 in HCC cell lines HCCLM3, HepG2, SMMC-7721, Hep3B and MHCC-97 was screened and the XB130 gene was inhibited. The expression of XB130 was detected by Western blotting and RT-PCR. Cell invasion ability was observed using Tranwell and cell migration ability was observed through cell scratches. Results Among the 90 HCC samples, the positive staining rate was 44.4% （40 cases）; among the positive staining samples, 29 cases were strongly positive and 11 cases were moderately to weakly positive. The study also found that among these samples, 24 cases were patients with recurrence. The expression of XB130 in HCC tissues was significantly higher than that in adjacent tissues （P < 0.05）. The higher the expression of XB130 in HCC, the shorter the postoperative survival time and the higher recurrence rate of patients （P < 0.05）. The relative expression level, cell invasion and migration ability of XB130 were decreased after inhibiting XB130 gene （P < 0.05）. Conclusion XB130 is expected to become a new prognostic marker of HCC. Down-regulating the expression of XB130 in HCC cells can alleviate its invasion and migration.
- Hepatocellular carcinoma,
- Novel adaptor protein XB130,
- Histopathology,
- Cell invasion,
- Cell migration

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