Expression of XB130 in Hepatocellular Carcinoma and Its Effect on Cell Invasion and Migration

Song-xu QI; Cheng QIU; Rong-jun CHEN; Zhan-hui CHEN; Wan-yu ZHANG; Shi-long TANG; Fan CAO; Chun-man LI; Nan XIE

doi:10.12259/j.issn.2095-610X.S20210413

Volume 42 Issue 4

Apr. 2021

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2021 > 42(4): 67-72

Song-xu QI, Cheng QIU, Rong-jun CHEN, Zhan-hui CHEN, Wan-yu ZHANG, Shi-long TANG, Fan CAO, Chun-man LI, Nan XIE. Expression of XB130 in Hepatocellular Carcinoma and Its Effect on Cell Invasion and Migration[J]. Journal of Kunming Medical University, 2021, 42(4): 67-72. doi: 10.12259/j.issn.2095-610X.S20210413

Citation:

Song-xu QI, Cheng QIU, Rong-jun CHEN, Zhan-hui CHEN, Wan-yu ZHANG, Shi-long TANG, Fan CAO, Chun-man LI, Nan XIE. Expression of XB130 in Hepatocellular Carcinoma and Its Effect on Cell Invasion and Migration[J]. Journal of Kunming Medical University, 2021, 42(4): 67-72. doi: 10.12259/j.issn.2095-610X.S20210413

Citation:

Song-xu QI, Cheng QIU, Rong-jun CHEN, Zhan-hui CHEN, Wan-yu ZHANG, Shi-long TANG, Fan CAO, Chun-man LI, Nan XIE. Expression of XB130 in Hepatocellular Carcinoma and Its Effect on Cell Invasion and Migration[J]. Journal of Kunming Medical University, 2021, 42(4): 67-72. doi: 10.12259/j.issn.2095-610X.S20210413

PDF( 896 KB)

Expression of XB130 in Hepatocellular Carcinoma and Its Effect on Cell Invasion and Migration

doi: 10.12259/j.issn.2095-610X.S20210413

1.
Dept. of Hepatology，Donghua Hospital of Sun Yat-sen University，Dongguan Guangdong 523000
2.
Dept. of Hepatopancreatobiliary Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2020-12-11
Publish Date: 2021-04-01

Abstract

Abstract

Objective To investigate the expression of new adaptor protein XB130 in hepatocellular carcinoma （HCC） tissues and adjacent tissues and its relationship with histopathology, and to explore its influence on the invasion and migration of hepatocellular carcinoma cells. Methods We collected and analyzed the pathological sections of stained HCC samples. The expression of XB130 in HCC cell lines HCCLM3, HepG2, SMMC-7721, Hep3B and MHCC-97 was screened and the XB130 gene was inhibited. The expression of XB130 was detected by Western blotting and RT-PCR. Cell invasion ability was observed using Tranwell and cell migration ability was observed through cell scratches. Results Among the 90 HCC samples, the positive staining rate was 44.4% （40 cases）; among the positive staining samples, 29 cases were strongly positive and 11 cases were moderately to weakly positive. The study also found that among these samples, 24 cases were patients with recurrence. The expression of XB130 in HCC tissues was significantly higher than that in adjacent tissues （P < 0.05）. The higher the expression of XB130 in HCC, the shorter the postoperative survival time and the higher recurrence rate of patients （P < 0.05）. The relative expression level, cell invasion and migration ability of XB130 were decreased after inhibiting XB130 gene （P < 0.05）. Conclusion XB130 is expected to become a new prognostic marker of HCC. Down-regulating the expression of XB130 in HCC cells can alleviate its invasion and migration.
- Hepatocellular carcinoma,
- Novel adaptor protein XB130,
- Histopathology,
- Cell invasion,
- Cell migration

FullText(HTML)

References(19)

References

[1]	Torre la,Bray f,Siegel R L,et al. Global cancer statistics,2012.[J]. Ca-Cancer J Clin,2015,65(2):87-108. doi: 10.3322/caac.21262
[2]	Shiozaki A,Liu M. Roles of XB130,a novel adaptor protein,in cancer.[J]. J Clin Bioinforma,2011,1(1):10-14. doi: 10.1186/2043-9113-1-10
[3]	Zhang J,Park S I,Artime M C,et al. AFAP-110 is overexpressed in prostate cancer and contributes to tumorigenic growth by regulating focal contacts[J]. J Clin Invest,2007,117(10):2962-2973. doi: 10.1172/JCI30710
[4]	Shiozaki A,Kosuga T,Ichikawa D,et al. XB130 as an independent prognostic factor in human esophageal squamous cell carcinoma[J]. Ann Surg Oncol,2013,20(9):3140-3150. doi: 10.1245/s10434-012-2474-4
[5]	Lodyga M,Bai X H,Kapus A,et al. Adaptor protein XB130 is a Rac-controlled component of lamellipodia that regulates cell motility and invasion[J]. J Cell Sci,2010,123(23):4156-4169. doi: 10.1242/jcs.071050
[6]	Liu X,Cai H,Huang H,et al. The prognostic significance of apoptosis-related biological markers in Chinese gastric cancer patients[J]. PLoS One,2011,6(12):e29670. doi: 10.1371/journal.pone.0029670
[7]	Xu J D,Furuya T,Cao X X,et al. Loss of BCL2L10 protein expression as prognostic predictor for poor clinical outcome in gastric carcinoma[J]. Histopathology,2010,57(6):814-824. doi: 10.1111/j.1365-2559.2010.03720.x
[8]	Shiozaki A,Lodyga M,Bai X H,et al. XB130,a novel adaptor protein,promotes thyroid tumor growth[J]. Am J Pathol,2011,178(1):391-401. doi: 10.1016/j.ajpath.2010.11.024
[9]	Wang X,Wang R,Liu Z,et al. XB130 expression in human osteosarcoma:A clinical and experimental study[J]. Int J Clin Exp Pathol,2015,8(3):2565-2573.
[10]	Shi M,Zheng D,Sun L,et al. XB130 promotes proliferation and invasion of gastric cancer cells[J]. J Transl Med,2014,12(1):1-11. doi: 10.1186/1479-5876-12-1
[11]	Li N Y,Weber C E,Wai P Y,et al. An MAPK-dependent pathway induces epithelial-mesenchymal transition via Twist activation in human breast cancer cell lines[J]. Surgery,2013,154(2):404-410. doi: 10.1016/j.surg.2013.05.012
[12]	Shiozaki A,Shen G,Bai X,et al. XB130 mediates cancer cell proliferation and survival through multiple signaling events downstream of Akt[J]. PLoS One,2012,7(8):e43646. doi: 10.1371/journal.pone.0043646
[13]	Otsuki S,Inokuchi M,Enjoji M,et al. Vimentin expression is associated with decreased survival in gastric cancer[J]. Oncol Rep,2011,25(5):1235-1242.
[14]	Liu J,Hu Y,Hu W,et al. Expression and prognostic relevance of p21WAF1 in stage III esophageal squamous cell carcinoma[J]. Dis Esophagus,2012,25(1):67-71. doi: 10.1111/j.1442-2050.2011.01217.x
[15]	Li J,Sun W,Wei H,et al. Expression of XB130 in human ductal breast cancer[J]. Int J Clin Exp Pathol,2015,8(5):5300-5308.
[16]	Cunha I W,Carvalho K C,Martins W K,et al. Identification of genes associated with local aggressiveness and Metastatic behavior in soft tissue tumors[J]. Transl Oncol,2010,3(1):23-32. doi: 10.1593/tlo.09166
[17]	Zhang J,Jiang X,Zhang J. Prognostic significance of XB130 expression in surgically resected pancreatic ductal adenocarcinoma[J]. World J Surg Oncol,2014,12(1):49-53. doi: 10.1186/1477-7819-12-49
[18]	Xu J,Bai XH,Lodyga M,et al. XB130,a novel adaptor protein for signal transduction[J]. J Biol Chem,2007,282(22):16401-16412. doi: 10.1074/jbc.M701684200
[19]	Zuo Q,Huang H,Shi M,et al. Multivariate analysis of several molecular markers and clinicopathological features in postoperative prognosis of hepatocellular carcinoma[J]. Anat Rec(Hoboken),2012,295(3):423-431. doi: 10.1002/ar.21531

Relative Articles(20)

Relative Articles

[1]	Qingbin ZENG, Rong Xu, Wenzhi DONG, Zhijian CHEN, Weiran LIAO, Kui LONG. Expression of Zinc Finger Transcription Factor Sall4 In Pancreatic Cancer and Its Impact on Cell Invasion and Migration. Journal of Kunming Medical University, 2024, 46(): 1-7.
[2]	Xiaohua LU, Hongxin YUAN. The Analysis of the Correlation between BTLA，CTLA-4 Gene Polymorphisms and the Efficacy and Prognosis of TACE Combined Targeted Therapy for Liver Cancer. Journal of Kunming Medical University, 2023, 44(9): 126-135. doi: 10.12259/j.issn.2095-610X.S20230927
[3]	Xuesha ZHANG, Jie ZHOU, Cailin LI, Dingyu WU, Fang PENG. Effects of Periplaneta americana Extract on Autophagy and Invasive Metastasis of Subcutaneous Transplanted Hepatocellular Carcinoma in Nude Mice. Journal of Kunming Medical University, 2023, 44(10): 39-46. doi: 10.12259/j.issn.2095-610X.S20231018
[4]	Liqiang LAN, Jing LI, Qingbo REN. Value Evaluation of New Markers AFP-L3 and PIVKAⅡ in the Diagnosis of Liver Cancer. Journal of Kunming Medical University, 2023, 44(7): 136-141. doi: 10.12259/j.issn.2095-610X.S20230705
[5]	Zhenhuan MA, Zhen LI, Xianglin ZHOU, Guojian LI, Guokai YANG, Jia WAN, Lingjuan DU, Yong YANG. Iodine-125 Seed Inhibits the Proliferation and Invasion of Gastric Cancer Cells by Regulating MicroRNA-193b-5p Expression. Journal of Kunming Medical University, 2022, 43(1): 8-13. doi: 10.12259/j.issn.2095-610X.S20220120
[6]	Heng ZHANG, Cheng ZHANG, Weiran LIAO, Yutao HE, Yuyang ZHANG, Bin DU, Lin WANG. Research Progress of Zinc Finger Protein and Hepatocellular Carcinoma. Journal of Kunming Medical University, 2022, 43(1): 144-149. doi: 10.12259/j.issn.2095-610X.S20220135
[7]	Shun-hui YIN, Jian-zhong ZHOU, Zi-liang LI. Tiger17 Promotes the Proliferation and Migration of Human Oral Mucosa Fibroblast Cells. Journal of Kunming Medical University, 2021, 42(8): 128-131. doi: 10.12259/j.issn.2095-610X.S20210823
[8]	Si-yu CHEN, Shuai FU, Yong WU. Effect of Gossypol Acetate Acid on Invasion of Human Tongue Squamous Cell Carcinoma Cal-27 Cells in vitro. Journal of Kunming Medical University, 2020, 41(11): 12-17. doi: 10.12259/j.issn.2095-610X.S20201101
[9]	Zhu Rong Song , Wei Xiao Ping , Dai Bin , Fan Hai Jun , Tan Yi Zheng , Fan Xue Rong , Tian Da Guang . . Journal of Kunming Medical University, 2019, 40(05): 68-72.
[10]	Xie Huan , Tian Jie , Huang Ya Fen . . Journal of Kunming Medical University, 2019, 40(05): 18-23.
[11]	Liu Xing . Research Advance in the Role of Toll-like Receptors in Hepatocarcinogenesis. Journal of Kunming Medical University,
[12]	Zhu Wei . Clinical and Pathological Analysis on 286 Cases of Seborrheic Keratosis. Journal of Kunming Medical University,
[13]	He Hai Yu . The Expression of GP73 in Serum and Tissues of Patients with Chronic Hepatitis B，Liver Cirrhosis and Primary Live r Cancer. Journal of Kunming Medical University,
[14]	Guo Tao . . Journal of Kunming Medical University,
[15]	Liu Li Xin . . Journal of Kunming Medical University,
[16]	Jiang Wei Guo . . Journal of Kunming Medical University,
[17]	Jiang Wei Guo . . Journal of Kunming Medical University,
[18]	Zhao Yu Min . . Journal of Kunming Medical University,
[19]	Ping Jing . . Journal of Kunming Medical University,
[20]	Yuan Hui . . Journal of Kunming Medical University,