Effects of Different Concentrations of Magnesium Ions on Endothelial Progenitor Cells in Women with Gestational Diabetes

Yan-shang ZHANG; Li LI; Xin-ling WANG; Su-xin LIU; Guo-juan MA; Yan HUO

doi:10.12259/j.issn.2095-610X.S20210416

Volume 42 Issue 4

Apr. 2021

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Yan-shang ZHANG, Li LI, Xin-ling WANG, Su-xin LIU, Guo-juan MA, Yan HUO. Effects of Different Concentrations of Magnesium Ions on Endothelial Progenitor Cells in Women with Gestational Diabetes[J]. Journal of Kunming Medical University, 2021, 42(4): 84-90. doi: 10.12259/j.issn.2095-610X.S20210416

Citation:

Yan-shang ZHANG, Li LI, Xin-ling WANG, Su-xin LIU, Guo-juan MA, Yan HUO. Effects of Different Concentrations of Magnesium Ions on Endothelial Progenitor Cells in Women with Gestational Diabetes[J]. Journal of Kunming Medical University, 2021, 42(4): 84-90. doi: 10.12259/j.issn.2095-610X.S20210416

Citation:

Yan-shang ZHANG, Li LI, Xin-ling WANG, Su-xin LIU, Guo-juan MA, Yan HUO. Effects of Different Concentrations of Magnesium Ions on Endothelial Progenitor Cells in Women with Gestational Diabetes[J]. Journal of Kunming Medical University, 2021, 42(4): 84-90. doi: 10.12259/j.issn.2095-610X.S20210416

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Effects of Different Concentrations of Magnesium Ions on Endothelial Progenitor Cells in Women with Gestational Diabetes

doi: 10.12259/j.issn.2095-610X.S20210416

Dept.of Obstetrics，Hebei Provincial People’s Hospital Shijiazhuang，Shijiazhuang Hebei 050051，China

Received Date: 2020-12-26
Publish Date: 2021-04-01

Abstract

Abstract

Objective To explore the effects of different concentrations of magnesium ion intervention on endothelial progenitor cells （endothelial progenitor cells, EPCs） in women with gestational diabetes （gestaional diabetes mellitus, GDM）. Methods 204 cases of gestational diabetes mellitus from May 2016 to March 2020 were selected as the observation group, and 204 cases of normal pregnant women hospitalized during the same period were selected as the control group. The observation group was divided into group A （4 mmol/L）, group B （8 mmol/L） and group C （16 mmol/L） by random number table method, and were treated with different concentrations of magnesium ions. Using the same method, the control group was divided into group a （4 mmol/L）, group b （8 mmol/L） and group c （16 mmol/L）, and was also given different concentrations of magnesium ions. There were 68 patients in each of the six groups. In the six groups of patients, each pregnant woman was enrolled for cord blood isolation and culture, and each case was enrolled with 6-well cells. The cell proliferation, apoptosis, adhesion and cell cycle of EPCs were compared. Results The proliferative capacity of the three groups of women in the observation group was lower than the those of control group, with group B better than group A, group A better than group C, group b better than group a, and group a better than group c. The apoptosis rate is 100% in normal population. The proliferative capacity of the three groups of women in the observation group is higher than that in the control group, with group B lower than group A, group A lower than group C, group b lower than group a, group a lower than group c, the difference is statistically significant （P < 0.05）. According to the calculation using Image J software, the cell area and cell density in the observation group were lower than those in the control group, with group B higher than group A, group A higher than group C, group b higher than group a, and group a higher than group c; the difference is statistically significant （P < 0.05）. The occurrence of G0 / G1 phase cell cycle arrest in the observation group was lower than that in control group, with group B better than group A, group A better than group C, group b better than group a, and group a better than group c, the difference is statistically significant （P < 0.05）. Conclusion 8 mmol/L magnesium ion may be associated with the proliferation and adhesion of EPCs in pregnant women, thus inhibiting apoptosis and upregulating cell cycle, and its effect on women with GDM is more evident, which can be used to help improve the pregnancy outcome of pregnant women with GDM after further study.
- Gestational diabetes,
- Endothelial progenitor cells,
- Magnesium ion

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