The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells

Lan YANG; Xiao JIA; Yi-tong JIANG; Qi CUI; Guang-ci LIU; Ying-hong HE

doi:10.12259/j.issn.2095-610X.S20210504

Volume 42 Issue 5

May 2021

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2021 > 42(5): 18-23

Lan YANG, Xiao JIA, Yi-tong JIANG, Qi CUI, Guang-ci LIU, Ying-hong HE. The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells[J]. Journal of Kunming Medical University, 2021, 42(5): 18-23. doi: 10.12259/j.issn.2095-610X.S20210504

Citation:

Lan YANG, Xiao JIA, Yi-tong JIANG, Qi CUI, Guang-ci LIU, Ying-hong HE. The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells[J]. Journal of Kunming Medical University, 2021, 42(5): 18-23. doi: 10.12259/j.issn.2095-610X.S20210504

Citation:

Lan YANG, Xiao JIA, Yi-tong JIANG, Qi CUI, Guang-ci LIU, Ying-hong HE. The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells[J]. Journal of Kunming Medical University, 2021, 42(5): 18-23. doi: 10.12259/j.issn.2095-610X.S20210504

PDF( 952 KB)

The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells

doi: 10.12259/j.issn.2095-610X.S20210504

Lan YANG^{1, 2},
Xiao JIA^{1, 2},
Yi-tong JIANG¹,
Qi CUI¹,
Guang-ci LIU¹,
Ying-hong HE^{1, 2
,
,}

1.
School of Basic Medicine of Dali University，Dali Yunnan 671000
2.
Key Laboratory of University Cell Biology，Yunnan Province，Dali Yunnan 671000，China

Received Date: 2021-01-15
Available Online: 2021-06-02
Publish Date: 2021-05-20

Abstract

Abstract

Objective To investigate the influence of the silence of UBE2C gene on the gastric cancer AGS cells proliferation and migration of human. Methods Using RNA interference technology, the expression vector of UBE2C silenced lentivirus was transfered into 293T cell line with packaging plasmids to produce the lentiviral particles. Lentivirus was used to infect human gastric cancer AGS cells, and the cells with stably expressing UBE2C were obtained by puromycin screening. The experiment was divided into the interference group and the interference control group. The expression levels of UBE2C mRNA in human gastric cancer AGS cells were detected by quantitative real-time polymerase chain reaction （qRT-PCR）. The capacity of AGS cell proliferation and migration was determined with real-time label free cell analyzer （RTCA）. Meanwhile, Cell counting kit-8 （CCK-8） was used to further confirm the effect of UBE2C expression level on the proliferation of human gastric cancer AGS cells. Results Compared with the interference control group, UBE2C shRNA transfected human gastric cancer AGS cells significantly decreased the expression of UBE2C mRNA （P < 0.05）; the cellular proliferation and migration ability were obviously decreased. Conclusion Targeted silencing of UBE2C gene expression can inhibit the malignant biological behavior of human gastric cancer AGS cells.
- UBE2C,
- human gastric cancer AGS cells,
- proliferation,
- Migration

FullText(HTML)

References(13)

References

[1]	Etemadi A,Safiri S,Sepanlou S. G,et al. The global,regional,and national burden of stomach cancer in 195 countries,1990-2017:A systematic analysis for the Global Burden of Disease study 2017[J]. Lancet Gastroenterol Hepatol,2020,5(1):42-54. doi: 10.1016/S2468-1253(19)30328-0
[2]	王静雷,杨一兵,耿云霞,等. 1990-2017年中国胃癌发病,患病及死亡状况趋势分析[J]. 中国慢性病预防与控制,2020,28(5):321-325.
[3]	A. Williamson,K. E. Wickliffe,B. G. Mellone,et al. Identification of a physiological E2 module for the human anaphase-promoting complex[J]. Proc. Natl. Acad. Sci. U. S. A,2009,106(43):18213-18218. doi: 10.1073/pnas.0907887106
[4]	M. K. Summers,B. Pan,K. Mukhyala,et al. The unique N terminus of the UbcH10 E2 enzyme controls the threshold for APC activation and enhances checkpoint regulation of the APC[J]. Mol. Cell,2008,31(4):544-556. doi: 10.1016/j.molcel.2008.07.014
[5]	Townsley F M,Aristarkhov A,Beck S,et al. Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase[J]. Proceedings of the National Academy of Sciences of the United States of America,1997,94(6):2362-2367. doi: 10.1073/pnas.94.6.2362
[6]	Yanagida M. Basic mechanism of eukaryotic chromosome segregation. Philosophical[J]. Transactions Biological Sciences,2005,360(1455):609-621. doi: 10.1098/rstb.2004.1615
[7]	Reddy S K,Rape M,Margansky W A,et al. Ubiquitination by the anaphase-promoting complex drives spindle checkpoint inactivation[J]. Nature,2007,446(7138):921-925. doi: 10.1038/nature05734
[8]	Okamoto Y,Ozaki T,Kou M,et al. UbcH10 is the cancer-related E2 ubiquitin-conjugating enzyme[J]. Cancer Research,2003,63(14):4167-4173.
[9]	Guo J,Wu Y,Du J,et al. Deregulation of UBE2C-mediated autophagy repression aggravates NSCLC progression[J]. Oncogenesis,2018,7(6):49-64. doi: 10.1038/s41389-018-0054-6
[10]	Wang R,Song Y,Liu X,et al. UBE2C induces EMT through Wnt/βcatenin and PI3K/Akt signaling pathways by regulating phosphorylation levels of Aurora-A[J]. International Journal of Oncology,2017,50(4):1116-1126. doi: 10.3892/ijo.2017.3880
[11]	A Z J,B X Z,A L C,et al. UBE2C promotes the progression of head and neck squamous cell carcinoma[J]. Biochemical and Biophysical Research Communications,2020,523(2):389-397. doi: 10.1016/j.bbrc.2019.12.064
[12]	Mo C H,Gao L,Zhu X F,et al. The clinicopathological significance of UBE2C in breast cancer:A study based on immunohistochemistry,microarray and RNA-sequencing data[J]. Cancer Cell International,2017,17(1):83-100. doi: 10.1186/s12935-017-0455-1
[13]	魏震,苌婉梅,郭小雨,等. UBE2C在结直肠癌中的研究现状与进展[J]. 现代肿瘤医学,2019,27(4):144-147.

Relative Articles(20)

Relative Articles

[1]	Yuru ZHAI, Yan BAI, Yunyun LI. FGF2 Regulates Hypoxia-Induced Proliferation and Collagen Metabolism of Scleral Fibroblasts Through the PERK/EIF2α/ATF4 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(10): 22-28. doi: 10.12259/j.issn.2095-610X.S20241004
[2]	Ling WANG, Xiangchuan QIN, Jinqiu LI, Hasim AXIANGU. CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome. Journal of Kunming Medical University, 2024, 45(1): 15-21. doi: 10.12259/j.issn.2095-610X.S20240103
[3]	Xiangchuan QIN, Jinqiu LI, Xiaojing HUANG, Kuerban HUTUBIDING·, Hasim AXIANGU·. Effect of HPV E6 on Proliferation，Invasion and Migration of Cervical Cancer Cells Through Rap1 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(9): 9-16. doi: 10.12259/j.issn.2095-610X.S20240902
[4]	Yelin ZHANG, Liya MA, Xuhui PENG, Hefeng YANG, Rui SHE. Hsa-let-7a-5p Regulates Proliferation and Apoptosis of Periodontal Ligament Stem Cells. Journal of Kunming Medical University, 2023, 44(10): 47-54. doi: 10.12259/j.issn.2095-610X.S20231028
[5]	Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells. Journal of Kunming Medical University, 2023, 44(9): 7-14. doi: 10.12259/j.issn.2095-610X.S20230926
[6]	Bangqing LIU, Jianfeng LI, Xiaohui LIU, Jinnan ZHANG, Jinping LIANG. MiR-196b Promotes Proliferation and Migration of Lung Adenocarcinoma by Targeting ERG. Journal of Kunming Medical University, 2023, 44(10): 83-91. doi: 10.12259/j.issn.2095-610X.S20231023
[7]	Li ZHANG, Qingcen WANG, Luohui ZHOU, Juan YANG, Hong WANG. Luteolin Inhibits HASMCs Migration by Endoplasmic Reticulum Stress. Journal of Kunming Medical University, 2022, 43(5): 12-17. doi: 10.12259/j.issn.2095-610X.S20220518
[8]	Wei ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Liang ZHANG. miR-125b-5p Regulates HK2 to Inhibit Proliferation and Glycolysis of Gallbladder Cancer Cells. Journal of Kunming Medical University, 2022, 43(12): 23-29. doi: 10.12259/j.issn.2095-610X.S20221206
[9]	Zhoujun LIAO, Shaohua YANG, Lixin LIU, Sheng HU, Yihui CHEN, Qiang KANG, Xiaowen ZHANG. Effect of AK4 on Proliferation and Migration of Intrahepatic Bile Duct Carcinoma Cell HUCCT1. Journal of Kunming Medical University, 2022, 43(6): 1-6. doi: 10.12259/j.issn.2095-610X.S20220611
[10]	Bin ZHAO, Yuanpeng DUAN, Guoying ZHANG, Chengwei BI, Libo YANG, Zhiyu SHI, Yong YANG, Jianpeng ZHANG, Ting GAO. CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p. Journal of Kunming Medical University, 2022, 43(7): 25-32. doi: 10.12259/j.issn.2095-610X.S20220731
[11]	Ai-ping XU, Hua LIN, Li-hui GAO, Ling LI, Meng-wei CHENG, Ge WANG, Juan YANG, Yan-fen NIU. Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1. Journal of Kunming Medical University, 2021, 42(7): 1-6. doi: 10.12259/j.issn.2095-610X.S20210701
[12]	Li Shan Shan , Quan Yu Hang , Gong Ling Li , Yang Hui , Pu Jing Hong , Wang Zhong Hui . . Journal of Kunming Medical University, 2020, 41(05): 103-107.
[13]	Pang Yue Wen , Gong Hui Jie , Wang Ling , Shi Jing , Duan JunYao , Feng Ying Ying , Yan Yong Ji . . Journal of Kunming Medical University, 2019, 40(08): 22-27.
[14]	Wei Dong . Effect of Fragile Site WWOX Gene on Regulating Proliferation of Human Gallbladder Cancer Cells in Vitro. Journal of Kunming Medical University,
[15]	Jia Nan Nan . Effect of Gnaq on the Proliferation of SH-SY5Y Cells and Its Mechanism of Action. Journal of Kunming Medical University,
[16]	Liu Li Xin . . Journal of Kunming Medical University,
[17]	Shi Zhao Kun . . Journal of Kunming Medical University,
[18]	Wang Hai Feng . . Journal of Kunming Medical University,
[19]	Liu Jia Xin . . Journal of Kunming Medical University,
[20]	Wu Bin . . Journal of Kunming Medical University,