Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands

Hong-fei ZHANG; De-long GE; Xiao-fei HUANG; Di MEI; Li GUO; Yi-rong WANG; Hai-mei LI; Tong-yi SUN; Guang-tao JIA; Wen-hui LI

doi:10.12259/j.issn.2095-610X.S20210507

Volume 42 Issue 5

May 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(5): 35-40

Hong-fei ZHANG, De-long GE, Xiao-fei HUANG, Di MEI, Li GUO, Yi-rong WANG, Hai-mei LI, Tong-yi SUN, Guang-tao JIA, Wen-hui LI. Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands[J]. Journal of Kunming Medical University, 2021, 42(5): 35-40. doi: 10.12259/j.issn.2095-610X.S20210507

Citation:

Hong-fei ZHANG, De-long GE, Xiao-fei HUANG, Di MEI, Li GUO, Yi-rong WANG, Hai-mei LI, Tong-yi SUN, Guang-tao JIA, Wen-hui LI. Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands[J]. Journal of Kunming Medical University, 2021, 42(5): 35-40. doi: 10.12259/j.issn.2095-610X.S20210507

Citation:

Hong-fei ZHANG, De-long GE, Xiao-fei HUANG, Di MEI, Li GUO, Yi-rong WANG, Hai-mei LI, Tong-yi SUN, Guang-tao JIA, Wen-hui LI. Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands[J]. Journal of Kunming Medical University, 2021, 42(5): 35-40. doi: 10.12259/j.issn.2095-610X.S20210507

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Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands

doi: 10.12259/j.issn.2095-610X.S20210507

1.
School of Biological Science and Technology，Weifang Medical University，Weifang Shandong 261053
2.
Kunming Institute of Zoology，Kunming Yunnan 650223，China

Received Date: 2021-01-08
Available Online: 2021-06-02
Publish Date: 2021-05-20

Abstract

Abstract

Objective To obtain the transcriptome dataset of Buthus martensii. Methods Taking the characteristic Buthus martensii in Linyi area of Shandong Province as the research object, the second-generation high-throughput sequencing platform Illumina HiSeq 4000 150PE was used to sequence the transcriptome of the anterior abdomen, posterior abdomen and venom gland of Buthus martensii. The venom gland genes were compared with the preabdomen and the postabdomen gene sets, and then the intersection of the two comparison was analyzed. The screening differential gene sets were analyzed by GO and KO. Results There were 60 major categories of GO functional classification, containing 23 biological pathways, 13 cellular components, and 24 molecular functions. Among the first 20 KEGG pathways that were most significantly enriched, the calcium ion signaling pathway and phospholipase signaling pathway were closely related to toxins. Conclusion In this study, we have constructed the transcriptome sequence database of B. martensii by nonparticipating transcriptome sequencing, which provids the sequence basis for the future research on the gene mining function of Buthus martensii and the research basis for the biosynthesis of metabolites.
- Buthus martensii,
- Venom gland,
- Preabdomen,
- Postabdomen,
- Transcriptome sequencing

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