Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands

Hong-fei ZHANG; De-long GE; Xiao-fei HUANG; Di MEI; Li GUO; Yi-rong WANG; Hai-mei LI; Tong-yi SUN; Guang-tao JIA; Wen-hui LI

doi:10.12259/j.issn.2095-610X.S20210507

Volume 42 Issue 5

May 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(5): 35-40

Hong-fei ZHANG, De-long GE, Xiao-fei HUANG, Di MEI, Li GUO, Yi-rong WANG, Hai-mei LI, Tong-yi SUN, Guang-tao JIA, Wen-hui LI. Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands[J]. Journal of Kunming Medical University, 2021, 42(5): 35-40. doi: 10.12259/j.issn.2095-610X.S20210507

Citation:

Hong-fei ZHANG, De-long GE, Xiao-fei HUANG, Di MEI, Li GUO, Yi-rong WANG, Hai-mei LI, Tong-yi SUN, Guang-tao JIA, Wen-hui LI. Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands[J]. Journal of Kunming Medical University, 2021, 42(5): 35-40. doi: 10.12259/j.issn.2095-610X.S20210507

Citation:

Hong-fei ZHANG, De-long GE, Xiao-fei HUANG, Di MEI, Li GUO, Yi-rong WANG, Hai-mei LI, Tong-yi SUN, Guang-tao JIA, Wen-hui LI. Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands[J]. Journal of Kunming Medical University, 2021, 42(5): 35-40. doi: 10.12259/j.issn.2095-610X.S20210507

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Traditional Chinese Medicine Transcriptome Analysis of Characteristic Distribution of Scorpion Genes in Venomous Glands

doi: 10.12259/j.issn.2095-610X.S20210507

1.
School of Biological Science and Technology，Weifang Medical University，Weifang Shandong 261053
2.
Kunming Institute of Zoology，Kunming Yunnan 650223，China

Received Date: 2021-01-08
Available Online: 2021-06-02
Publish Date: 2021-05-20

Abstract

Abstract

Objective To obtain the transcriptome dataset of Buthus martensii. Methods Taking the characteristic Buthus martensii in Linyi area of Shandong Province as the research object, the second-generation high-throughput sequencing platform Illumina HiSeq 4000 150PE was used to sequence the transcriptome of the anterior abdomen, posterior abdomen and venom gland of Buthus martensii. The venom gland genes were compared with the preabdomen and the postabdomen gene sets, and then the intersection of the two comparison was analyzed. The screening differential gene sets were analyzed by GO and KO. Results There were 60 major categories of GO functional classification, containing 23 biological pathways, 13 cellular components, and 24 molecular functions. Among the first 20 KEGG pathways that were most significantly enriched, the calcium ion signaling pathway and phospholipase signaling pathway were closely related to toxins. Conclusion In this study, we have constructed the transcriptome sequence database of B. martensii by nonparticipating transcriptome sequencing, which provids the sequence basis for the future research on the gene mining function of Buthus martensii and the research basis for the biosynthesis of metabolites.
- Buthus martensii,
- Venom gland,
- Preabdomen,
- Postabdomen,
- Transcriptome sequencing

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References(24)

References

[1]	李钟杰,吴文澜,王勇. 东亚钳蝎多肽资源的药理活性研究进展[J]. 中草药,2018,49(21):5179-5190. doi: 10.7501/j.issn.0253-2670.2018.21.033
[2]	毛歆,刘辉,李文鑫,等. 马氏正钳蝎的形态和结构特征[J]. 动物学杂志,2017,52(3):468-477.
[3]	Chippaux J P,Goyffon M. Epidemiology of scorpionism:A global appraisal[J]. Acta Tropica,2008,107(2):71-79. doi: 10.1016/j.actatropica.2008.05.021
[4]	杨彬,高文远,张艳军. 基于转录组学-蛋白质组学-多肽组学整合关联分析策略的动物药蛋白多肽类成分研究思路及方法[J]. 中草药,2019,50(5):1033-1038. doi: 10.7501/j.issn.0253-2670.2019.05.001
[5]	王巍杰,王涛. 蓖麻基因组毒素家族的生物信息学分析[J]. 基因组学与应用生物学,2017,36(8):3083-3090.
[6]	张博,苏杭,任献青,等. 基于高通量转录组测序的菟丝子黄酮改善雷公藤多苷片致大鼠生殖损伤的机制研究[J]. 中国中药杂志,2019,44(16):3478-3485.
[7]	吴昕怡,严媛,刘小莉. 基于高通量测序的青叶胆转录组研究[J]. 中国现代应用药学,2018,35(3):363-369.
[8]	吴超,彭娟,向林,等. 基于高通量测序的铁皮石斛叶片转录组分析[J]. 分子植物育种,2016,14(12):3334-3346.
[9]	Grabherr M G,Haas B J,Yassour M,et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome[J]. Nature Biotechnology,2011,29(7):644-652. doi: 10.1038/nbt.1883
[10]	贾岩,张福生,肖淑贤,等. 款冬花不同发育阶段的代谢组学和比较转录组学分析[J]. 中国生物化学与分子生物学报,2017,33(6):615-623.
[11]	冯陈晨,王文龙,红梅,等. 骆驼斯氏副柔线虫雌虫和雄虫比较转录组学分析[J]. 中国农业大学学报,2017,22(7):61-70. doi: 10.11841/j.issn.1007-4333.2017.07.008
[12]	江灵敏,王碧霞,孟蕾. 小叶女贞叶片转录组研究[J]. 中国现代应用药学,2018,35(11):1670-1674.
[13]	Ali Mortazavi,Brian A Williams,Kenneth McCue,et al. Mapping and quantifying mammalian transcriptomes by RNA-Seq[J]. Nature Methods,2008,5(7):621-628. doi: 10.1038/nmeth.1226
[14]	Ye J,Fang L,Zheng H,et al. WEGO:A web tool for plotting GO annotations[J]. Nucleic Acids Res,2006,34(2):293-297.
[15]	Anders S,Huber W. Differential expression analysis for sequence count data[J]. Genome Biol,2010,11(10):R106. doi: 10.1186/gb-2010-11-10-r106
[16]	Conesa A,Gotz S,et al. Blast2GO:A universal tool for annotation,visualization and analysis in functional genomics research[J]. Bioinformatics,2005,21(18):3674-3676. doi: 10.1093/bioinformatics/bti610
[17]	罗辉,叶华,肖世俊,等. 转录组学技术在水产动物研究中的运用[J]. 水产学报,2015,39(4):598-607.
[18]	马赛箭,安超,薛文娇,等. 基于高通量测序的出芽短梗霉转录组学研究[J]. 基因组学与应用生物学,2016,35(4):931-941.
[19]	蒋可人,马峥,郑航,等. 转录组与蛋白质组整合分析在生物学研究中的应用[J]. 生物技术通报,2018,34(12):50-55.
[20]	展波,高媛媛,林维平,等. 中华大蟾蜍皮肤Cathelicidin家族新型抗菌肽的鉴定及其抗菌活性[J]. 中国中药杂志,2016,41(4):630-635.
[21]	RA Dueñas-Cuellar,Santana C,ACM Magalhães,et al. Scorpion toxins and ion channels:Potential applications in cancer therapy[J]. Toxins,2020,12(5):326-327. doi: 10.3390/toxins12050326
[22]	陈惠,辛丽丽,龚婕宁. 基于全转录组测序技术的转录组学在中医药领域的应用前景分析[J]. 环球中医药,2013,6(10):759-763. doi: 10.3969/j.issn.1674-1749.2013.10.014
[23]	张晓萌,李健春,王琼,等. 转录组测序技术在中医药领域的应用[J]. 中国现代中药,2016,18(8):1084-1087.
[24]	肖炜卓,张龙,王晓冬,等. 慢性牙周炎患者龈下菌斑宏转录组学分析[J]. 中国医药导报,2019,16(21):21-23.

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