Application of 16SrRNA Gene Detection in the Early Diagnosis of Bacterial Meningitis in Children

Shu-lan SHI; Xi-ting DAI; Guang-zhou ZHAO; Xiao Juan LI; Rong-jie LI; Ming-biao MA

doi:10.12259/j.issn.2095-610X.S20210522

Volume 42 Issue 5

May 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(5): 120-125

Shu-lan SHI, Xi-ting DAI, Guang-zhou ZHAO, Xiao Juan LI, Rong-jie LI, Ming-biao MA. Application of 16SrRNA Gene Detection in the Early Diagnosis of Bacterial Meningitis in Children[J]. Journal of Kunming Medical University, 2021, 42(5): 120-125. doi: 10.12259/j.issn.2095-610X.S20210522

Citation:

Shu-lan SHI, Xi-ting DAI, Guang-zhou ZHAO, Xiao Juan LI, Rong-jie LI, Ming-biao MA. Application of 16SrRNA Gene Detection in the Early Diagnosis of Bacterial Meningitis in Children[J]. Journal of Kunming Medical University, 2021, 42(5): 120-125. doi: 10.12259/j.issn.2095-610X.S20210522

Citation:

Shu-lan SHI, Xi-ting DAI, Guang-zhou ZHAO, Xiao Juan LI, Rong-jie LI, Ming-biao MA. Application of 16SrRNA Gene Detection in the Early Diagnosis of Bacterial Meningitis in Children[J]. Journal of Kunming Medical University, 2021, 42(5): 120-125. doi: 10.12259/j.issn.2095-610X.S20210522

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Application of 16SrRNA Gene Detection in the Early Diagnosis of Bacterial Meningitis in Children

doi: 10.12259/j.issn.2095-610X.S20210522

1.
Yunnan Children’s Medical Center，Clinical Laboratory of Kunming Children’s Hospital，Kunming Yunnan 650228
2.
Clinical Laboratory of Yunnan Cancer Hospital，Kunming Yunnan 650118，China

Received Date: 2021-02-11
Available Online: 2021-06-08
Publish Date: 2021-05-20

Abstract

Abstract

Objective To explore the value of 16SrRNA gene detection in cerebrospinal fluid （CSF） in the early diagnosis of bacterial meningitis （BM） in children. Methods CSF specimens were collected from 40 patients with BM who were diagnosed of BM in Kunming Children’ s Hospital between January 2019 and June 2020. PCR was used to detect the 16SrRNA gene in the specimens. Then the 16SrRNA gene was sequenced for the positive samples by PCR, and the sequencing results sequence alignment and homology were analyzed by NCBI BLAST. At the same time, all the specimens were cultured simultaneously. Results Of the 40 children’ s CSF samples, 16 were positive for 16SrRNA gene PCR, with a positive rate of 40%; 7 cases were positive for bacterial culture, with a positive rate of 17.5%. The positive rate of PCR method was higher than that of the bacterial culture method （χ² = 4.93, P < 0.05）, with CSF as the “gold standard”, the sensitivity and specificity of PCR assay were 71.4% （5/7） and 66.7% （22/33）; the results of 16SrRNA gene sequencing were consistent with the results of CSF culture, and five bacterias which were not detected by CSF culture; the time of CSF culture was （61.21±12.62） h, however, the PCR detection time required （7.09±0.45） h, and the difference of detection time between the two methods was statistically significant （P < 0.05）. Conclusion The PCR detection method of 16SrRNA gene in CSF can improve the detection rate of CSF pathogenic bacteria in BM patients, and it can reduce the missed detection rate. It has the specific and rapid characteristics and can provide the reliable pathogenic basis for early diagnosis of clinical BM in time.
- 16SrRNA gene,
- Bacterial meningitis,
- Cerebrospinal fluid culture,
- Polymerase chain reaction,
- Early diagnosis,
- Children

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References(16)

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