PNS Promotes the Wound-healing of Superficial Second-degree Burn Rats by Regulating PDGF-BB/PDGFR-β Expression

Li ZHANG; Jian-yu YANG; Yan YANG; Ren-hua YANG; Zhi-qiang SHEN; Wen-ying CHAI; Peng CHEN

doi:10.12259/j.issn.2095-610X.S20210639

Volume 42 Issue 6

Jul. 2021

Turn off MathJax

Article Contents

Abstract

References

Relative Articles

Article Navigation > Journal of Kunming Medical University > 2021 > 42(6): 7-15

Dongyan YANG, Hua LIN, Yuxiao ZHANG, Kunying ZHAO, Lirong XIANG, Shuda YANG, Weiyan HU. Efficacy and Safety of Bevacizumab Combined with Temozolomide for Recurrent High-Grade Glioma[J]. Journal of Kunming Medical University, 2024, 45(4): 67-73. doi: 10.12259/j.issn.2095-610X.S20240410

Citation:

Li ZHANG, Jian-yu YANG, Yan YANG, Ren-hua YANG, Zhi-qiang SHEN, Wen-ying CHAI, Peng CHEN. PNS Promotes the Wound-healing of Superficial Second-degree Burn Rats by Regulating PDGF-BB/PDGFR-β Expression[J]. Journal of Kunming Medical University, 2021, 42(6): 7-15. doi: 10.12259/j.issn.2095-610X.S20210639

Citation:

PDF( 1022 KB)

PNS Promotes the Wound-healing of Superficial Second-degree Burn Rats by Regulating PDGF-BB/PDGFR-β Expression

doi: 10.12259/j.issn.2095-610X.S20210639

1.
School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products
2.
School of Basic Medical Science，Kunming Medical University，Kunming Yunnan 650500
3.
Dept. of Breast Surgery First Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650031，PR China

Received Date: 2021-03-06
Available Online: 2021-07-03
Publish Date: 2021-07-21

Abstract

Abstract

Objective To observe the therapeutic of PNS on wound healing and the expression of PDGF-BB, PDGFR-β in rats superficial second-degree burn model, and to explore the underlying mechanism of PNS on second-degree burn wound healing. Methods Rats with superficial second-degree burn were treated with PNS, applying HE staining to detect burn severity, and then biopsied biological tissue on days 0, 7, 14, 21 after administration, respectively. Moreover, Immunohistochemistry （IHC）, Western blot and RT-PCR methods were used to detect protein and mRNA expression levels of PDGF-BB/PDGFR-β in skin tissues after treatment with PNS, respectively. Furthermore, the skin wound healing at different time points was monitored by macroscopic observation. Results HE staining suggested that the replication of rats second-degree burn model was successful. Macro monitoring data demonstrates that compared with model group, the removal time of scab and fur-growthing were shortened, and the wound healing rate was increased in the PNS high-dose group （P < 0.05）. Combination of IHC, WB and RT-PCR showed that the expression levels of PDGF-BB/PDGFR-β increased sharply on day 7, maintaining a high level until day 14, approaching normal levels on day 21 after PNS treatment. However, the protein and mRNA expressions of PDGF-BB/PDGFR-β showed high values on day 21 in the model group, which was a slow process. Our results suggest that compared with model group, the peak expression of PDGF-BB/PDGFR-β are earlier, shortening of fur-growthing and decrustation time, and improvement of wound healing rate in rats superficial second-degree burn model. Conclusion PNS can significantly promote skin wound healing in rats superficial second-degree burn model, and its mechanism is closely related to the up-regulation of the expression levels of PDGF-BB/PDGFR-β in skin tissue.
- PNS,
- Rats,
- Superficial second-degree burn model,
- PDGF-BB/PDGFR-β

FullText(HTML)

References(16)

References

[1]	Matthew P Rowan,Leopoldo C Cancio,Eric A Elster,et al. Burn wound healing and treatment:review and advancements[J]. Crit Care,2015,6(19):243-251.
[2]	Xu Y,Tan H Y,Li S,et al. Panax notoginseng for inflammation-related chronic diseases:A review on the modulations of multiple pathways[J]. The American Journal of Chinese Medicine,2018,46(5):1-26.
[3]	Li Qing-rong,WANG Zi yu. Progress in pharmacological action of panax notoginseng saponins[J]. Hunan Journal of Traditional Chinese Medicine,2017,33(9):216-218.
[4]	Wang Ting,Guo Ri-xin,Zhou Guo-hong,et al. Traditional uses,botany,phytochemistry,pharmacology and toxicology of Panaxnotoginseng(Burk.)F. H. Chen:Areview[J]. Journal of Ethnopharmacology,2016,88(1):234-258.
[5]	Yang Zong-cheng. Chinese Burn Surgery [M]. Beijing: People’s Medical Publishing House, 2016: 345-380.
[6]	Reinke J M,Sorg H. Wound repair and regeneration[J]. Eur Surg Res,2012,49(1):35-43. doi: 10.1159/000339613
[7]	Giri P,Ebert S,Braumann U. Skin regeneration in deep second-degree scald injuries either by infusion pumping,or topical application of recombinant human erythropoietin gel[J]. Drug Des Devel Ther,2015,9(3):2565-2579.
[8]	Fu Ying-ping,Sun Qian-hong,Yang Jan-yu,et al. An experimental research on the treatment of burn rats with PPE compound preparation[J]. Journal of Kunming Medical University,2010,2(6):11-15.
[9]	Teng Jia,Guo Hui Yang Jan-yu,et al. Experimental research on the treatment of cautery injury in mice by compound preparation[J]. Yunnan Journal of Traditional Chinese Medicine,2009,30(7):57-58.
[10]	Andrade A L M,Brassolatti P,Luna G F,et al. Effect of photobiomodulation associated with cell therapy in the process of cutaneous regeneration in third degree burns in rats[J]. Tissue Eng Regen Med,2020,14(5):673-683. doi: 10.1002/term.3028
[11]	Luo G X. Mechanism and prevention of organ complications post burn injury[J]. Zhong hua Shao Shang Za Zhi,2019,35(8):565-567.
[12]	Xu C,Wang W,Wang B,et al. Analytical methods and biological activities of Panax notoginseng saponins:Recent trends[J]. J Ethnopharmacol,2019,23(236):443-465.
[13]	Kimura Y,Sumiyoshi M,Kawahira K,et al. Effects of ginseng saponins isolated from Red Ginseng roots on burn wound healing in mice[J]. British Journal of Pharmacology,2006,148(6):860-70. doi: 10.1038/sj.bjp.0706794
[14]	Bayir Y,Un H,Ugan R A,et al. The effects of Beeswax,Olive oil and Butter impregnated bandage on burn wound healing[J]. Burns,2019,45(6):1410-1417. doi: 10.1016/j.burns.2018.03.004
[15]	Kondo T,Ishida Y. Molecular pathology of wound healing[J]. Forensic Sci Int,2010,203(3):93-98.
[16]	Martin P. Wound healing—Aiming for perfect skin regeneration[J]. Science,1997,276(5309):75-81. doi: 10.1126/science.276.5309.75

Relative Articles(20)

Relative Articles

[1]	Yiting TAO, Ge BAI, Dan YU, Zhu ZHOU, Miao LI. A Comparative Study on the Establishment of Animal Models of Chronic Renal Failure by Two Methods. Journal of Kunming Medical University, 2024, 45(12): 28-34. doi: 10.12259/j.issn.2095-610X.S20241204
[2]	Linjuan YANG, Wenjiao JIN, Jinzhi LU, Zhenghua XIONG, Bin YAN, Xuesong HAN. A Comparative Study of Rat Models of Intrauterine Adhesions Constructed in Different Ways. Journal of Kunming Medical University, 2023, 44(3): 7-14. doi: 10.12259/j.issn.2095-610X.S20230322
[3]	Le ZHAN, Ruiyu MA, Ni WAN, Liwen MO, Huiying CHEN, Li ZHANG. Effects of Puerarin on Th17/Treg Cell Immune Homeostasis and the Expression of Related Transcription Factors in Rats with Periodontitis. Journal of Kunming Medical University, 2022, 43(11): 36-43. doi: 10.12259/j.issn.2095-610X.S20221129
[4]	Shibing QIAN, Mingyao MENG, Hongbin YU, Kaiwen DUAN, Changquan LI, Zhigang XIA. Effect of Panax Noto-ginseng Saponins on the Proliferation of Human Stem Cells from Apical Papilla. Journal of Kunming Medical University, 2022, 43(10): 22-27. doi: 10.12259/j.issn.2095-610X.S20221018
[5]	Xia-rong GONG, Li-gong BIAN, Yu-hui CHEN, Jing CHEN, Bo WANG, Guo-li BI. A Study on Diffusion Kurtosis Imaging in Acute Epilepsy Rats. Journal of Kunming Medical University, 2021, 42(4): 28-32. doi: 10.12259/j.issn.2095-610X.S20210405
[6]	Liu YANG, Qi-cai QU, Rui CHEN, Shu-lan JIAO, Yong-yu SI, Hua ZHOU. Effects of Dexmedetomidine on Inflammatory Response in Rats with Smoke Inhalation Lung Injury. Journal of Kunming Medical University, 2020, 41(11): 30-37. doi: 10.12259/j.issn.2095-610X.S20201124
[7]	Ke Jin , Cheng Xin , Hou An Guo . Preparation and in Vitro Release Study of Panax Notoginseng Sustained-release Pellets. Journal of Kunming Medical University, 2017, 38(09): 24-29.
[8]	Tian Wei . Liraglutide Improves The Symptoms of Non-alcoholic Fatty Liver Disease by Regulating Fatty Acid β-oxidation. Journal of Kunming Medical University,
[9]	Zhong Lian Mei . The Activation and Polarization of Microglia in Epileptic Rats Induced by Pilocarpine. Journal of Kunming Medical University,
[10]	Li Yu Huan . . Journal of Kunming Medical University,
[11]	Chang Lu . . Journal of Kunming Medical University,
[12]	Tian Jin Tao . . Journal of Kunming Medical University,
[13]	Xu Lin Cheng . . Journal of Kunming Medical University,
[14]	Xiong Yu Xin . . Journal of Kunming Medical University,
[15]	Zeng Yu Jian . . Journal of Kunming Medical University,
[16]	Li Hong Li . . Journal of Kunming Medical University,
[17]	Li Jun Yan . . Journal of Kunming Medical University,
[18]	Lan Wei Dong . . Journal of Kunming Medical University,
[19]	Li Hua . . Journal of Kunming Medical University,
[20]	Zhou Lei . . Journal of Kunming Medical University,