PNS Promotes the Wound-healing of Superficial Second-degree Burn Rats by Regulating PDGF-BB/PDGFR-β Expression

Li ZHANG; Jian-yu YANG; Yan YANG; Ren-hua YANG; Zhi-qiang SHEN; Wen-ying CHAI; Peng CHEN

doi:10.12259/j.issn.2095-610X.S20210639

Volume 42 Issue 6

Jul. 2021

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Li ZHANG, Jian-yu YANG, Yan YANG, Ren-hua YANG, Zhi-qiang SHEN, Wen-ying CHAI, Peng CHEN. PNS Promotes the Wound-healing of Superficial Second-degree Burn Rats by Regulating PDGF-BB/PDGFR-β Expression[J]. Journal of Kunming Medical University, 2021, 42(6): 7-15. doi: 10.12259/j.issn.2095-610X.S20210639

Citation:

Li ZHANG, Jian-yu YANG, Yan YANG, Ren-hua YANG, Zhi-qiang SHEN, Wen-ying CHAI, Peng CHEN. PNS Promotes the Wound-healing of Superficial Second-degree Burn Rats by Regulating PDGF-BB/PDGFR-β Expression[J]. Journal of Kunming Medical University, 2021, 42(6): 7-15. doi: 10.12259/j.issn.2095-610X.S20210639

Citation:

Li ZHANG, Jian-yu YANG, Yan YANG, Ren-hua YANG, Zhi-qiang SHEN, Wen-ying CHAI, Peng CHEN. PNS Promotes the Wound-healing of Superficial Second-degree Burn Rats by Regulating PDGF-BB/PDGFR-β Expression[J]. Journal of Kunming Medical University, 2021, 42(6): 7-15. doi: 10.12259/j.issn.2095-610X.S20210639

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PNS Promotes the Wound-healing of Superficial Second-degree Burn Rats by Regulating PDGF-BB/PDGFR-β Expression

doi: 10.12259/j.issn.2095-610X.S20210639

1.
School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products
2.
School of Basic Medical Science，Kunming Medical University，Kunming Yunnan 650500
3.
Dept. of Breast Surgery First Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650031，PR China

Received Date: 2021-03-06
Available Online: 2021-07-03
Publish Date: 2021-07-21

Abstract

Abstract

Objective To observe the therapeutic of PNS on wound healing and the expression of PDGF-BB, PDGFR-β in rats superficial second-degree burn model, and to explore the underlying mechanism of PNS on second-degree burn wound healing. Methods Rats with superficial second-degree burn were treated with PNS, applying HE staining to detect burn severity, and then biopsied biological tissue on days 0, 7, 14, 21 after administration, respectively. Moreover, Immunohistochemistry （IHC）, Western blot and RT-PCR methods were used to detect protein and mRNA expression levels of PDGF-BB/PDGFR-β in skin tissues after treatment with PNS, respectively. Furthermore, the skin wound healing at different time points was monitored by macroscopic observation. Results HE staining suggested that the replication of rats second-degree burn model was successful. Macro monitoring data demonstrates that compared with model group, the removal time of scab and fur-growthing were shortened, and the wound healing rate was increased in the PNS high-dose group （P < 0.05）. Combination of IHC, WB and RT-PCR showed that the expression levels of PDGF-BB/PDGFR-β increased sharply on day 7, maintaining a high level until day 14, approaching normal levels on day 21 after PNS treatment. However, the protein and mRNA expressions of PDGF-BB/PDGFR-β showed high values on day 21 in the model group, which was a slow process. Our results suggest that compared with model group, the peak expression of PDGF-BB/PDGFR-β are earlier, shortening of fur-growthing and decrustation time, and improvement of wound healing rate in rats superficial second-degree burn model. Conclusion PNS can significantly promote skin wound healing in rats superficial second-degree burn model, and its mechanism is closely related to the up-regulation of the expression levels of PDGF-BB/PDGFR-β in skin tissue.
- PNS,
- Rats,
- Superficial second-degree burn model,
- PDGF-BB/PDGFR-β

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References(16)

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