Study on Anxious Behaviors of Pure and Multiple Concussion on the 14th Day Post-injury in Rats

Zhen WANG; Xue HAN; Wen YU; Zhen-zhen CAO; Huan ZHANG; Li YANG; Jian-yun YU

doi:10.12259/j.issn.2095-610X.S20210647

Volume 42 Issue 6

Jul. 2021

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Zhen WANG, Xue HAN, Wen YU, Zhen-zhen CAO, Huan ZHANG, Li YANG, Jian-yun YU. Study on Anxious Behaviors of Pure and Multiple Concussion on the 14th Day Post-injury in Rats[J]. Journal of Kunming Medical University, 2021, 42(6): 1-6. doi: 10.12259/j.issn.2095-610X.S20210647

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Zhen WANG, Xue HAN, Wen YU, Zhen-zhen CAO, Huan ZHANG, Li YANG, Jian-yun YU. Study on Anxious Behaviors of Pure and Multiple Concussion on the 14th Day Post-injury in Rats[J]. Journal of Kunming Medical University, 2021, 42(6): 1-6. doi: 10.12259/j.issn.2095-610X.S20210647

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Study on Anxious Behaviors of Pure and Multiple Concussion on the 14th Day Post-injury in Rats

doi: 10.12259/j.issn.2095-610X.S20210647

1.
Forensic Medical College of Kunming Medical University
2.
Dept. of Emergency，The First affiliated Hospital of Kunming Medical University
3.
Basic medical experimental teaching center
4.
Dept. of Human Anatomy and Histology and Embryology，Kunming Yunnan 650500，China

Received Date: 2021-04-11
Available Online: 2021-07-03
Publish Date: 2021-07-21

Abstract

Abstract

Objective To investigate the effects of pure and multiple cerebral concussion （MCC） on the changes of anxiety behaviors in rats. Methods There were 50 adult SD rats with single pendulum closed brain injury device to simulate pure concussion （PCC） and multiple concussion （MCC）, and a normal control group （N） with 12 rats in each group. Open field test （OFT） and high plus maze （HPM） were performed on the 14th day after injury to assess changes in anxious behaviours. Results （1） In the OFT experiment: ① In the PCC group and the 3 MCC group, the walking number in the CA was （9.500 （6.50, 16.75）, （5.00 （3.50, 9.00）, and the N group was （10.00 （7.00, 17.88）. The number of lattice walking and residence time in the central area in the injury group were both less than those in the control group, and the difference between the 3 MCC group and the N group and the PCC group was statistically significant （P < 0.05）（P = 0.024, P = 0.033）. ② In the PCC group and the 3 MCC group, the walking number in the SA was （54.50 （26.88, 62.25））, （65.00 （28.50, 81.00））, and the N group was （33.00 （1.13, 51.50））. The number of surround areas （SA） and walking time in the injury group were all higher than those in the control group, and the difference between the 3 MCC group and the N group was statistically significant （P < 0.05, P = 0.015）. ③ The frequency of combing hair in PCC group and 3 MCC group was （4.20±1.03） times, （2.44±0.73） times, and N group was （5.20±1.62） times.The number of grooming times in the injury group was less than that in the N group, and the difference between the 3 MCC group and the N group and the PCC group was statistically significant （P < 0.05, P = 0.013, P = 0.019）. ④ in the open field experiment, the number of walking lattice, walking time and the number of grooming in the CA area of rats in each injury group showed a decreasing trend with the increase of the number of blows.In addition, with the increase of the number of strikes, the number of walking lattice and walking time in SA area of injured rats presented an increasing trend.（2） In the HMP experiment: ① The number of OA in the PCC group and the 3 MCC group was （1.00 （0.00, 1.00）） times, （0.50 （0.00, 1.00）） times, and the N group was （1.00 （0.00, 2.00）） times. The Times and time of entering open arms （OA） in each injury group were less than those in the normal group, but the differences between groups were not statistically significant, P > 0.05. ② the number of times of exploration in OA in the injury group was less than that in the N group, and the difference of 3 MCC group was statistically significant （P < 0.05, P = 0.032）. ③ The number of EA in the PCC group and the 3 MCC group was （1.00 （1.00, 1.00））times, （0.00 （0.00, 1.00）） times, and the N group was （0.00 （0.00, 1.00）） times. The times and time of the lesion group entering the closed arm （EA） was higher than that of the N group, The number of EA entry was statistically significant between the 3 MCC group and the PCC group （P < 0.05, P = 0.015）. At the time of EA, there was a statistically significant difference between the 3 MCC group, N group and PCC group （P < 0.05, P = 0.042, P = 0.027）. ④ In the overhead cross maze experiment, the number of rats entering the OA arm, the time and the number of visits to the floor all decreased with the increase of the number of blows. In addition, with the increase of batting times, the residence time of injured rats in the EA arm increased. Conclusion The anxious behaviors of rats with PCC and MCC increased significantly on the 14th day after injury, and the anxious behaviors of rats with MCC are more serious than that with PCC.
- Multiple cerebral concussion,
- Anxious behavior,
- Open field test,
- High plus maze

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References(14)

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