Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1

Ai-ping XU; Hua LIN; Li-hui GAO; Ling LI; Meng-wei CHENG; Ge WANG; Juan YANG; Yan-fen NIU

doi:10.12259/j.issn.2095-610X.S20210701

Volume 42 Issue 7

Jul. 2021

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2021 > 42(7): 1-6

Ai-ping XU, Hua LIN, Li-hui GAO, Ling LI, Meng-wei CHENG, Ge WANG, Juan YANG, Yan-fen NIU. Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1[J]. Journal of Kunming Medical University, 2021, 42(7): 1-6. doi: 10.12259/j.issn.2095-610X.S20210701

Citation:

Ai-ping XU, Hua LIN, Li-hui GAO, Ling LI, Meng-wei CHENG, Ge WANG, Juan YANG, Yan-fen NIU. Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1[J]. Journal of Kunming Medical University, 2021, 42(7): 1-6. doi: 10.12259/j.issn.2095-610X.S20210701

Citation:

Ai-ping XU, Hua LIN, Li-hui GAO, Ling LI, Meng-wei CHENG, Ge WANG, Juan YANG, Yan-fen NIU. Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1[J]. Journal of Kunming Medical University, 2021, 42(7): 1-6. doi: 10.12259/j.issn.2095-610X.S20210701

PDF( 1283 KB)

Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1

doi: 10.12259/j.issn.2095-610X.S20210701

Biomedical Engineering Research Center of Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2021-05-19
Publish Date: 2021-07-25

Abstract

Abstract

Objective To study the effects of norathyriol on epithelial-mesenchymal transition of HK-2 cells induced by TGF-β1. Methods MTT assay was used to detect the effect of norathyriol on HK-2 cell viability. The HK-2 cells were divided into normal group, model group, norathyriol （10^-8, 10^-7, 10^-6 mol·L^-1） groups and positive control （benzbromarone, 10^-7 mol/L） group. The norathyriol and benzbromarone were incubated for 4 h before HK-2 cells were treated by TGF-β1 （10 ng/mL） to induce EMT. Then, the HK-2 cells were cultured for another 24 h or 48 h. Scratch assay was used to detect the migration motility of HK-2 cells. Transwell invasion assay was used to detect invasion ability of HK-2 cells. The proteins expression of fibronectin （FN） and collagen type Ⅰ （ColⅠ） were detected by western blot. Results （1） The norathyriol （10^-9-10^-5 mol/L）has no effects on the HK-2 cell viability, compared with the normal group （P > 0.05）. （2） Compared with the normal group, the cells morphology of the model group changed from the original cobblestone shape to a long fusiform shape, the migration rate and the invasive ability of the model group was significantly increased （ P < 0.01）. The protein expression of Col and FN in the model group was significantly increased. Compared with the model group, the cells morphology of norathyriol （10 ^-8, 10^-7, 10^-6 mol/L） and benzbromarone （10^-7 mol/L） groups maintain thecobblestone shape partly, the migration rate （10^-8, 10^-7, 10^-6 mol/L） and the invasive ability （10^-6 mol/L） of norathyriol were significantly decreased （P < 0.01）; The protein expression of Col and FN were significantly down-regulated in the norathyriol （10 ^-6 mol/L） group and the benzbromarone group （P < 0.05, 0.01）. Conclusion Norathyriol can inhibit the migration and invasion of HK-2 cells induced by TGF-β1 and down-regulate the protein expression of fibronectin （FN） and collagen type Ⅰ （ColⅠ）.
- Norathyriol,
- TGF-β1,
- HK-2 cell,
- Migration,
- Invasion,
- Epithelial-mesenchymal transition （EMT）

FullText(HTML)

References(14)

References

[1]	Liu B C,Tang T T,Lv L L,et al. Renal tubule injury:A driving force toward chronic kidney disease[J]. Kidney international,2018,93(3):568-579. doi: 10.1016/j.kint.2017.09.033
[2]	Djudiai S,Boor P. Cellular and molecular mechanisms of kidney fibrosis[J]. Molecular Aspects of Medicine,2019,65:16-36. doi: 10.1016/j.mam.2018.06.002
[3]	石咏琪,王墨. 肾间质纤维化发生机制研究进展[J]. 现代医药卫生,2019,35(19):3002-3006. doi: 10.3969/j.issn.1009-5519.2019.19.020
[4]	Cruz-Solbes A S,Youker K. Epithelial to mesenchymal transition (EMT) and endothelial to mesenchymal transition (EndMT):role and implications in kidney fibrosis[J]. Results and Problems in Cell Differentiation,2017,60:345-372.
[5]	Iwano M,Plieth D,Danoff T M,et al. Evidence that fibroblasts derive from epithelium during tissue fibrosis[J]. The Journal of Clinical Investigation,2002,110(3):341-350. doi: 10.1172/JCI0215518
[6]	遆安航,陈瑾,翟康欣,等. 连翘归尾煎对人乳腺癌MCF-7细胞增殖、迁移、凋亡和周期的影响[J]. 山西医科大学学报,2021,52(5):565-571.
[7]	Saral L,Michael Z,Raghu K. Partial epithelial-to-mesenchymal transition and other new mechanisms of kidney fibrosis[J]. Trends in Endocrinology and Metabolism,2016,27(10):681-695. doi: 10.1016/j.tem.2016.06.004
[8]	Meng X M,Nikolic-paterson D J,Lan H Y. TGF-β:the master regulator of fibrosis[J]. Nature Reviews. Nephrology,2016,12(6):325-338. doi: 10.1038/nrneph.2016.48
[9]	Xu C G,Zhu X L,Wang W,et al. Ursolic acid inhibits epithelial-mesenchymal transition in vitro and in vivo[J]. Pharmaceutical Biology,2019,57(1):169-175. doi: 10.1080/13880209.2019.1577464
[10]	Fan Y,Wang X,Li Y,et al. PAK4 enhances TGF-β1-induced epithelial- mesenchymal transition through activating β-catenin signaling pathway in renal tubular epithelial cells[J]. International Journal of Clinical and Experimental pathology,2018,11(6):3026-3035.
[11]	Song Y,Lv S S,Wang F,et al. Overexpression of BMP-7 reverses TGF-β1-induced epithelial-mesenchymal transition by attenuating the Wnt3/β-catenin and /Smad2/3 signaling pathways in HK-2 cells[J]. Molecular Medicine Reports,2019,21(2):833-841.
[12]	周栋,傅海燕. 细胞外基质的产生、重塑与肾脏纤维化[J]. 江苏医药,2015,41(8):936-938.
[13]	李淑菊,詹晓丹,田锋,等. 参地补肾胶囊含药血清对高糖诱导转分HK-2细胞ZO-1、α-SMA表达的影响[J]. 中国中医药科技,2018,25(6):797-800.
[14]	Louis K,Hertig A. How tubular epithelial cells dictate the rate of renal fibrogenesis?[J]. World Journal of Nephrology,2015,4(3):367-373. doi: 10.5527/wjn.v4.i3.367

Relative Articles(20)

Relative Articles

[1]	Xiaochuan YIN, Ruiyang YIN, Ranhua LI, Fangqi CAI, Yue CUI, Tao BI, Xinghe TONG. Culture of Malignant Pleural Mesothelioma Cells and the Effects of CDKN2B on Cancer Cell. Journal of Kunming Medical University, 2024, 45(1): 28-34. doi: 10.12259/j.issn.2095-610X.S20240105
[2]	Zhuohui LIU, Shiyin QIN, Hexiang ZHAO, Fengfeng JIA, Biao RUAN, Ruiqing LONG. Inhibitory Effect of Crocin on Pituitary Adenomas via IRF7/NF-κB Signaling Pathway. Journal of Kunming Medical University, 2024, 46(): 1-9.
[3]	Xiangchuan QIN, Jinqiu LI, Xiaojing HUANG, Kuerban HUTUBIDING·, Hasim AXIANGU·. Effect of HPV E6 on Proliferation，Invasion and Migration of Cervical Cancer Cells Through Rap1 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(9): 9-16. doi: 10.12259/j.issn.2095-610X.S20240902
[4]	Dongxing XU, Bo TANG, Guo ZHU, Xuefen LEI, Qiuhong WANG, Dong WEI. Effect and Mechanism of Twist1 Regulating Bmi1 on Invasion and Migration of Gallbladder Carcinoma Cells. Journal of Kunming Medical University, 2023, 44(3): 28-33. doi: 10.12259/j.issn.2095-610X.S20230320
[5]	Yao ZOU, Ziyu WANG, Jiao LI, Xingxin LI, Yaqiong SHEN, Jing XU, Yan JIN. Association between LOXL2 and Snail Expression Levels and Overall Survival Rate after Surgery in Patients with Cholangiocarcinoma. Journal of Kunming Medical University, 2022, 43(12): 81-87. doi: 10.12259/j.issn.2095-610X.S20221215
[6]	Yu’e JIANG, Zhaoming ZHONG, Yanzhang GAO, Wei WANG, Ruimei SUN, Chuanzheng SUN. Role and Mechanism of TGF β1 in Platelet Promoting Invasion and Migration of Anaplastic Thyroid Carcinoma Cells. Journal of Kunming Medical University, 2022, 43(12): 1-5. doi: 10.12259/j.issn.2095-610X.S20221201
[7]	Bin ZHAO, Yuanpeng DUAN, Guoying ZHANG, Chengwei BI, Libo YANG, Zhiyu SHI, Yong YANG, Jianpeng ZHANG, Ting GAO. CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p. Journal of Kunming Medical University, 2022, 43(7): 25-32. doi: 10.12259/j.issn.2095-610X.S20220731
[8]	Li ZHANG, Qingcen WANG, Luohui ZHOU, Juan YANG, Hong WANG. Luteolin Inhibits HASMCs Migration by Endoplasmic Reticulum Stress. Journal of Kunming Medical University, 2022, 43(5): 12-17. doi: 10.12259/j.issn.2095-610X.S20220518
[9]	Zhoujun LIAO, Shaohua YANG, Lixin LIU, Sheng HU, Yihui CHEN, Qiang KANG, Xiaowen ZHANG. Effect of AK4 on Proliferation and Migration of Intrahepatic Bile Duct Carcinoma Cell HUCCT1. Journal of Kunming Medical University, 2022, 43(6): 1-6. doi: 10.12259/j.issn.2095-610X.S20220611
[10]	Zhi-hong LI, Yong HU, Zheng-hua XIONG, Xi-cui LONG, Shao-bo LU, Xue-song HAN. Expression and Significance of TGF - β 1，TIMP-1 and Collagen Ⅲ in Vaginal Wall of Patients with Pelvic Organ Prolapse. Journal of Kunming Medical University, 2021, 42(9): 95-100. doi: 10.12259/j.issn.2095-610X.S20210909
[11]	Ping-fen YANG, Hua LIN, Li-hui GAO, Ling LI, Meng-wei CHENG, Juan YANG, Ge WANG, Yan-fen NIU. Effect of Norathyriol on Uric Acid Excretion Indicators in Hyperuricemic Nephropathy Rats. Journal of Kunming Medical University, 2021, 42(6): 16-21. doi: 10.12259/j.issn.2095-610X.S20210633
[12]	Lan YANG, Xiao JIA, Yi-tong JIANG, Qi CUI, Guang-ci LIU, Ying-hong HE. The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells. Journal of Kunming Medical University, 2021, 42(5): 18-23. doi: 10.12259/j.issn.2095-610X.S20210504
[13]	Li Shan Shan , Quan Yu Hang , Gong Ling Li , Yang Hui , Pu Jing Hong , Wang Zhong Hui . . Journal of Kunming Medical University, 2020, 41(05): 103-107.
[14]	Li Kang Jian , Luo Yu Hui , Mo Yin , Shen Ji Hong , Liu Xiao Dong , Li Hao . Small Interfering RNA Silencing VDR in HK-2 Cells and Its Significance for Hypocitraturia. Journal of Kunming Medical University, 2017, 38(10): 22-26.
[15]	Zhao Bin , Li Rui Qian , Zhang Guo Ying , Lei Yong Hong , Wang Qi Lin , Liu Zhi Min . Overexpression of miR-4328 Inhibits the Proliferation, Migration and Epithelial Mesenchymal Transition of Prostate Cancer Cells. Journal of Kunming Medical University, 2017, 38(11): 29-33.
[16]	Liang Nai Chao . Correlation between Expression of MMP-9， TIMP-1 and VEGF and the Invasion of Non Small Cell Lung Cancer. Journal of Kunming Medical University,
[17]	Liu Feng . . Journal of Kunming Medical University,
[18]	Shi Zhao Kun . . Journal of Kunming Medical University,
[19]	Liu Jia Xin . . Journal of Kunming Medical University,
[20]	. Influence of Hypoxia on Hepatoma Carcinoma Cell Line SMMC7721 in Vitro. Journal of Kunming Medical University,