Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1

Ai-ping XU; Hua LIN; Li-hui GAO; Ling LI; Meng-wei CHENG; Ge WANG; Juan YANG; Yan-fen NIU

doi:10.12259/j.issn.2095-610X.S20210701

Volume 42 Issue 7

Jul. 2021

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Ai-ping XU, Hua LIN, Li-hui GAO, Ling LI, Meng-wei CHENG, Ge WANG, Juan YANG, Yan-fen NIU. Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1[J]. Journal of Kunming Medical University, 2021, 42(7): 1-6. doi: 10.12259/j.issn.2095-610X.S20210701

Citation:

Ai-ping XU, Hua LIN, Li-hui GAO, Ling LI, Meng-wei CHENG, Ge WANG, Juan YANG, Yan-fen NIU. Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1[J]. Journal of Kunming Medical University, 2021, 42(7): 1-6. doi: 10.12259/j.issn.2095-610X.S20210701

Citation:

Ai-ping XU, Hua LIN, Li-hui GAO, Ling LI, Meng-wei CHENG, Ge WANG, Juan YANG, Yan-fen NIU. Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1[J]. Journal of Kunming Medical University, 2021, 42(7): 1-6. doi: 10.12259/j.issn.2095-610X.S20210701

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Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1

doi: 10.12259/j.issn.2095-610X.S20210701

Biomedical Engineering Research Center of Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2021-05-19
Publish Date: 2021-07-25

Abstract

Abstract

Objective To study the effects of norathyriol on epithelial-mesenchymal transition of HK-2 cells induced by TGF-β1. Methods MTT assay was used to detect the effect of norathyriol on HK-2 cell viability. The HK-2 cells were divided into normal group, model group, norathyriol （10^-8, 10^-7, 10^-6 mol·L^-1） groups and positive control （benzbromarone, 10^-7 mol/L） group. The norathyriol and benzbromarone were incubated for 4 h before HK-2 cells were treated by TGF-β1 （10 ng/mL） to induce EMT. Then, the HK-2 cells were cultured for another 24 h or 48 h. Scratch assay was used to detect the migration motility of HK-2 cells. Transwell invasion assay was used to detect invasion ability of HK-2 cells. The proteins expression of fibronectin （FN） and collagen type Ⅰ （ColⅠ） were detected by western blot. Results （1） The norathyriol （10^-9-10^-5 mol/L）has no effects on the HK-2 cell viability, compared with the normal group （P > 0.05）. （2） Compared with the normal group, the cells morphology of the model group changed from the original cobblestone shape to a long fusiform shape, the migration rate and the invasive ability of the model group was significantly increased （ P < 0.01）. The protein expression of Col and FN in the model group was significantly increased. Compared with the model group, the cells morphology of norathyriol （10 ^-8, 10^-7, 10^-6 mol/L） and benzbromarone （10^-7 mol/L） groups maintain thecobblestone shape partly, the migration rate （10^-8, 10^-7, 10^-6 mol/L） and the invasive ability （10^-6 mol/L） of norathyriol were significantly decreased （P < 0.01）; The protein expression of Col and FN were significantly down-regulated in the norathyriol （10 ^-6 mol/L） group and the benzbromarone group （P < 0.05, 0.01）. Conclusion Norathyriol can inhibit the migration and invasion of HK-2 cells induced by TGF-β1 and down-regulate the protein expression of fibronectin （FN） and collagen type Ⅰ （ColⅠ）.
- Norathyriol,
- TGF-β1,
- HK-2 cell,
- Migration,
- Invasion,
- Epithelial-mesenchymal transition （EMT）

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References(14)

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