Effectiveness of Low Intensity Focused Ultrasound Stimulation of Nucleus Accumbens in Mice

Yun-yan LAN; Yu-ran FENG; Yu ZHENG; Zhen-dong YANG; Yi ZHAO; Mei ZHU

doi:10.12259/j.issn.2095-610X.S20210703

Volume 42 Issue 7

Jul. 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(7): 13-19

Yun-yan LAN, Yu-ran FENG, Yu ZHENG, Zhen-dong YANG, Yi ZHAO, Mei ZHU. Effectiveness of Low Intensity Focused Ultrasound Stimulation of Nucleus Accumbens in Mice[J]. Journal of Kunming Medical University, 2021, 42(7): 13-19. doi: 10.12259/j.issn.2095-610X.S20210703

Citation:

Yun-yan LAN, Yu-ran FENG, Yu ZHENG, Zhen-dong YANG, Yi ZHAO, Mei ZHU. Effectiveness of Low Intensity Focused Ultrasound Stimulation of Nucleus Accumbens in Mice[J]. Journal of Kunming Medical University, 2021, 42(7): 13-19. doi: 10.12259/j.issn.2095-610X.S20210703

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Effectiveness of Low Intensity Focused Ultrasound Stimulation of Nucleus Accumbens in Mice

doi: 10.12259/j.issn.2095-610X.S20210703

Dept. of Ultrasound，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2021-04-26
Publish Date: 2021-07-25

Abstract

Abstract

Objective To investigate the effectiveness of ultrasound stimulation for neural regulation from the aspects of behavioral pathology and neurobiochemistry by using low intensity focused ultrasound to stimulate the nucleus accumbens of normal mice. Methods 100 C57BL/6 mice were randomly divided into experimental group and control group with 50 mice in each group. The control group was given sham stimulation, and the experimental group was given transcranial low-intensity focused ultrasound to stimulate the NAc, twice a day, 10 min each time, for 7 consecutive days. After the intervention, 30 mice were randomly assigned to behavioral assessment （forced swimming, open field, and water maze）, 10 mice were assigned to electron microscopy （EM）, and 10 mice were assigned to neurotransmitter test.The depression, anxiety and learning and memory ability of the mice were evaluated by forced swimming, open field and water maze tests.The ultrastructural changes of neurons in the NAcof mice were observed by transmission electron microscopy. The contents of monoamine neurotransmitters （norepinephrine, dopamine, 5-hydroxytryptamine） in NAc of mice were determined by ELISA. Results 1. Behavioral evaluation results: Compared with the control group, the immobile time in the ultrasonic stimulation group was significantly longer （P < 0.01）, the total distance travelled, the average speed travelled, the distancetravelled of the central area, the time of the central area and the number of exits from the central area in the open field all decreased significantly （ P < 0.05, P < 0.01）, In the water maze experiment, the avoidance latency time was significantly longer, the number of crossing the platform was significantly reduced, and the residence time in the target quadrant was significantly shorter （ P < 0.05, P < 0.01）. 2. Electron microscopy results: The ultrastructure ofNAc neurons in the control group was basically normal. In the ultrasound stimulation group, the neurons were swelling, nerve fiber dissolution and necrosis, mitochondrial vacuolation, endoplasmic reticulum dilation, and intracytoplasmic organelles were reduced or disappeared.3. Measurement results of neurotransmitters: Compared with the control group, the contents of NAc monoamine neurotransmitters NE, DA and 5-HT were significantly decreased in the ultrasonic stimulation group （ P < 0.05）. Conclusion Low intensity focused ultrasound can induce inhibitory neuroregulation in mice, and cause morphological and neurobiochemical changes in NAC neurons, which may be one of the mechanisms of ultrasound neuromodulation.
- Low intensity focused ultrasound,
- Neuromodulation,
- Nucleus accumbens,
- Effectiveness

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References(17)

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