Determination of Norfloxacin Glucose Injection in EDTA by RP-HPLC Method

Na-na RONG; YU-feng LI; Li-yue REN; Yue-xin HU

doi:10.12259/j.issn.2095-610X.S20210807

Volume 42 Issue 8

Aug. 2021

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Na-na RONG, YU-feng LI, Li-yue REN, Yue-xin HU. Determination of Norfloxacin Glucose Injection in EDTA by RP-HPLC Method[J]. Journal of Kunming Medical University, 2021, 42(8): 35-39. doi: 10.12259/j.issn.2095-610X.S20210807

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Determination of Norfloxacin Glucose Injection in EDTA by RP-HPLC Method

doi: 10.12259/j.issn.2095-610X.S20210807

1.
Yunnan Institute for Food and Drug Control，Kunming Yunnan 650106
2.
Center for Food and Drug Inspection of Yunnan Province，Kunming Yunnan 650106
3.
Science and Technology Achievement Incubation Center，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2021-06-08
Available Online: 2021-08-04
Publish Date: 2021-08-04

Abstract

Abstract

Objective To establish a method for determination of norfloxacin glucose injection in compound EDTA injection. Methods The chromatographic column was Shiseido C18-MGⅡ5 μm 4.6×250 mm, with the mobile phase of acetonitrile: 0.05 mol/L ammonium acetate: water, mobile phase ratio of 70∶15∶15, flow rate of 1.0 mL/min, injection volume of 20 L, column temperature of 35 ℃, detection wavelength of 254 nm. The chelate from ferric chloride and EDTA disodium reaction were determined. Results EDTA-2Na was detected without any interference. In the range and peak area of 2.0326 µg/mL-200.3260 µg/mL, EDTA-2Na showed a good linear relation （R² = 0.9997）, the average recovery was 98.11% （n = 9）. Conclusion The method is rapid, simple, specific, reproducible, accurate and reliable, and is suitable for determination of EDTA in norfloxacin glucose injection.
- High performance liquid chromatography,
- Norfloxacin glucose injection,
- EDTA-2Na,
- Determination of content

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References(20)

References

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