The Application Value of Dry Fluorescent Luminescence in The Diagnosis and Treatment of HBV Infection

Song-qin LV; Xi-ruo SUN; Shan HUANG; Li-ping XV; Juan ZHANG; Yi-na WANG; Xiao-fei LI; Yong-rui YANG

doi:10.12259/j.issn.2095-610X.S20210812

Volume 42 Issue 8

Aug. 2021

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Song-qin LV, Xi-ruo SUN, Shan HUANG, Li-ping XV, Juan ZHANG, Yi-na WANG, Xiao-fei LI, Yong-rui YANG. The Application Value of Dry Fluorescent Luminescence in The Diagnosis and Treatment of HBV Infection[J]. Journal of Kunming Medical University, 2021, 42(8): 65-70. doi: 10.12259/j.issn.2095-610X.S20210812

Citation:

Song-qin LV, Xi-ruo SUN, Shan HUANG, Li-ping XV, Juan ZHANG, Yi-na WANG, Xiao-fei LI, Yong-rui YANG. The Application Value of Dry Fluorescent Luminescence in The Diagnosis and Treatment of HBV Infection[J]. Journal of Kunming Medical University, 2021, 42(8): 65-70. doi: 10.12259/j.issn.2095-610X.S20210812

Citation:

Song-qin LV, Xi-ruo SUN, Shan HUANG, Li-ping XV, Juan ZHANG, Yi-na WANG, Xiao-fei LI, Yong-rui YANG. The Application Value of Dry Fluorescent Luminescence in The Diagnosis and Treatment of HBV Infection[J]. Journal of Kunming Medical University, 2021, 42(8): 65-70. doi: 10.12259/j.issn.2095-610X.S20210812

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The Application Value of Dry Fluorescent Luminescence in The Diagnosis and Treatment of HBV Infection

doi: 10.12259/j.issn.2095-610X.S20210812

1.
The 3rd People’s Hospital of Kunming/Clinical Center for Infectious Diseases in Yunnan Province，Kunming Yunnan 650041
2.
Shanghai Rongsheng Biology Pharmacy Company，Shanghai 201108，China

Received Date: 2021-06-18
Available Online: 2021-08-03
Publish Date: 2021-08-04

Abstract

Abstract

Objective To analyze and evaluate the application value of dry fluorescent luminescence in the diagnosis and treatment of HBV infection. Methods A total of 397 serum samples were collected from patients attending Liver Diseases Department of the Third People’ s Hospital of Kunming from June 1, 2020 to December 31, 2020. Among these samples, 360 were detected for HBsAg, HBsAb, HBeAg, HBeAb and HBcAb by dry fluorescent luminescence method and ELISA, and 37 HBsAg positive samples in HBeAg seroconversion period detected by electrochemiluminescence method were tested for HBsAg, HBeAg and HBeAb by three methods: dry fluorescent luminescence method, enzyme-linked immunosorbent assay and colloidal gold method. The positive coincidence rate, negative coincidence rate and total coincidence rate of each test item by dry fluorescent luminescence method in diagnosis and treatment of HBV infection were compared and analyzed. Results The S/C value of ELISA was consistent with the trend of S/C value of dry fluorescent luminescence method, showing a certain positive correlation （r = 0.819, 0.913, 0.906, 0.732, 0.842, P < 0.001）. The positive coincidence rate, negative coincidence rate and total coincidence rate of the five items of HBV by the two detection techniques were more than 90%, with high consistency （Kappa = 0.949, 0.944, 0.955, 0.842, 0.949）. The results of 37 serum samples from HBsAg positive HBeAg seroconversion period showed that the coincidence rate between dry fluorescent luminescence method and Roche electrochemiluminescence HBeAb was 100% （37/37）, much higher than that of ELISA 18.92% （7/37） and colloidal gold 5.40% （2/37）. Conclusion Dry fluorescent luminescence method can effectively reflect the serological conversion period of HBeAg. Conventional HBV screening test and enzyme-linked immunosorbent assay have a high consistency. At the same time, it has the advantages of short detection time, no frequent manual operation and simple operation, so it has a good application prospect.
- Dry fluorescent luminescence,
- Hepatitis B virus,
- Accuracy rate

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