The Rapid Detection of Nucleic Acid in SARS-CoV-2 by Reverse Transcription Loop Mediated Isothermal Amplification

Huan LI; Qiu-yue XU; Yun-juan WANG; Yang SU; Rui-zhu TANG

doi:10.12259/j.issn.2095-610X.S20210904

Volume 42 Issue 9

Sep. 2021

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2021 > 42(9): 25-31

Huan LI, Qiu-yue XU, Yun-juan WANG, Yang SU, Rui-zhu TANG. The Rapid Detection of Nucleic Acid in SARS-CoV-2 by Reverse Transcription Loop Mediated Isothermal Amplification[J]. Journal of Kunming Medical University, 2021, 42(9): 25-31. doi: 10.12259/j.issn.2095-610X.S20210904

Citation:

Huan LI, Qiu-yue XU, Yun-juan WANG, Yang SU, Rui-zhu TANG. The Rapid Detection of Nucleic Acid in SARS-CoV-2 by Reverse Transcription Loop Mediated Isothermal Amplification[J]. Journal of Kunming Medical University, 2021, 42(9): 25-31. doi: 10.12259/j.issn.2095-610X.S20210904

Citation:

Huan LI, Qiu-yue XU, Yun-juan WANG, Yang SU, Rui-zhu TANG. The Rapid Detection of Nucleic Acid in SARS-CoV-2 by Reverse Transcription Loop Mediated Isothermal Amplification[J]. Journal of Kunming Medical University, 2021, 42(9): 25-31. doi: 10.12259/j.issn.2095-610X.S20210904

PDF( 907 KB)

The Rapid Detection of Nucleic Acid in SARS-CoV-2 by Reverse Transcription Loop Mediated Isothermal Amplification

doi: 10.12259/j.issn.2095-610X.S20210904

Dept. of Clinical Laboratory，The 1st Affiliated Hospital of Kunming Medical University，Yunnan Institute of Experimental Diagnosis，Yunnan Key Laboratory of Laboratory Medicine，Kunming Yunnan 650032，China

Received Date: 2021-07-27
Available Online: 2021-09-09
Publish Date: 2021-09-30

Abstract

Abstract

Objective To establish a rapid detection method for the nucleic acid of severe acute respiratory syndrome coronavirus 2 （SARS-CoV-2） based on the reverse transcription loop mediated isothermal amplification （RT-LAMP）. Method The RT-LAMP primers were designed according to the conservative region of N and ORF1ab gene of SARS-CoV-2. And the specificity, the minimum detection limit, the anti-interference ability and the best reaction time of the system were evaluated. Result Artificial synthesized SARS-CoV-2 nucleic acid samples were tested, and the results were consistent with the expected results.It confirmed the feasibility of the detection system. Conclusion The reaction system using RT-LAMP for the rapid detection of SARS-CoV-2 nucleic acid has the high specificity and sensitivity, and is suitable for the border areas with limited conditions.

FullText(HTML)

References(14)

References

[1]	Zhou P,Yang X L,Wang X G,et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin[J]. Nature,2020,579(7798):270-273. doi: 10.1038/s41586-020-2012-7
[2]	Gorbalenya A E,Baker S C,Baric R S,et al. The species severe acute respiratory syndrome-related coronavirus:classifying 2019-nCoV and naming it SARS-CoV-2[J]. Nature Microbiology,2020,5(5):536-544.
[3]	Corman V M,Landt O,Kaiser M,Molenkamp R,et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR[J]. Euro Surveill,2020,25(3):2000045.
[4]	Wang Y,Kang H,Liu X,et al. Combination of RT-qPCR testing and clinical features for diagnosis of COVID-19 facilitates management of SARS-CoV-2 Outbreak[J]. J Med Virol,2020,92(6):538-539. doi: 10.1002/jmv.25721
[5]	Craw P,Balachandran W. Isothermal nucleic acid amplification technologies for point-of-care diagnostics:A critical review[J]. Lab Chip,2012,12(14):2469-2486. doi: 10.1039/c2lc40100b
[6]	Asiello P J,Baeumner A J. Miniaturized isothermal nucleic acid amplification,a review[J]. Lab Chip,2011,11(8):1420-1430. doi: 10.1039/c0lc00666a
[7]	匡慧慧,于梅,于帅,等. 新型冠状病毒实验室核酸检测方法及实践[J]. 中华医院感染学杂志,2020,30(6):830-833.
[8]	To K K,Tsang O T,Yip C C,et al. Consistent detection of 2019 novel coronavirus in saliva[J]. Clin Infect Dis,2020,71(15):841-843. doi: 10.1093/cid/ciaa149
[9]	Tomita N,Mori Y,Kanda H,et al. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products[J]. Nat Protoc,2008,3(5):877-882. doi: 10.1038/nprot.2008.57
[10]	Notomi T,Mori Y,Tomita N,et al. Loop-mediated isothermal amplification (LAMP):principle,features,and future prospects[J]. Journal of Microbiology,2015,53(1):1-5. doi: 10.1007/s12275-015-4656-9
[11]	匡燕云,李思光,罗玉萍. 环介导等温扩增核酸技术及其应用[J]. 微生物学通报,2007,34(3):557-560. doi: 10.3969/j.issn.0253-2654.2007.03.039
[12]	肖斌,朱永红,邹全明. 简便敏感的环介导等温扩增基因诊断新技术[J]. 中华检验医学杂志,2005,28(7):761-763. doi: 10.3760/j:issn:1009-9158.2005.07.034
[13]	Wang H,Li X,Li T,et al. The genetic sequence,origin,and diagnosis of SARS-CoV-2[J]. European Journal of Clinical Microbiology & Infectious Diseases,2020,39(9):1629-1635.
[14]	Hongwarittorrn I,Chaichanawongsaroj N,Laiwattanapaisal W. Semi-quantitative visual detection of loop mediated isothermal amplification (LAMP)-generated DNA by distance-based measurement on a paper device[J]. Talanta,2017,175(12):135-142.

Relative Articles(20)

Relative Articles

[1]	Songqin LYU, Shan HUANG, Litang MA, Xiu WANG, Hongtao ZHANG, Qin LI, Chunping WAN, Zhengchao LYU. The Investigation and Analysis of the Situation of COVID-19 Vaccination and Vaccination Willingness in HIV/AIDS Population in Yunnan Province. Journal of Kunming Medical University, 2024, 45(1): 48-54. doi: 10.12259/j.issn.2095-610X.S20240108
[2]	Haiping HE. Analysis of Coagulation Function Indexes in Elderly Patients with Novel Coronavirus Pneumonia. Journal of Kunming Medical University, 2024, 45(4): 99-104. doi: 10.12259/j.issn.2095-610X.S20240414
[3]	Ying HUANG, Yun LUO, Yan WU, Bing LIU, Shan JIANG, Hua HE. The Regularity and Clinical Manifestations of Throat Swab and Fecal Detoxification in New Coronavirus Infected Patients. Journal of Kunming Medical University, 2022, 43(1): 96-101. doi: 10.12259/j.issn.2095-610X.S20220112
[4]	Dong-ling LI, Li-hua LI, Dong PU, Hong-ying WANG, Ting-ting YU, Jing BAI. The Detection Results of Viral Nucleic Acid in Various Biological Samples of Patients with COVID-19 in Kunming. Journal of Kunming Medical University, 2022, 43(1): 1-6. doi: 10.12259/j.issn.2095-610X.S20220106
[5]	Hanqing ZHAO, Shibing QIAN, Mengqi WANG, Rongyu YANG, Kaiwen DUAN. Oral Lesions Associated with 2019-nCoV. Journal of Kunming Medical University, 2022, 43(8): 156-160. doi: 10.12259/j.issn.2095-610X.S20220831
[6]	Li WU, Yun-hui YANG, Jing ZHANG, Zhang-cong YANG, Yu SU, Yong-jian WEN, Yu-chun WU, Peng WANG, Zhi-peng LI, Wei ZHAO, Dan HAN, Rui-dong WANG, Bo HE. The Correlation between the Imaging Characteristics of Imported COVID-19 at First Diagnosis and Clinical Severity Grading. Journal of Kunming Medical University, 2021, 42(7): 49-56. doi: 10.12259/j.issn.2095-610X.S20210708
[7]	Yun-hui YANG, Bo HE, Ding-yun YOU, Jun-tao YANG, Qian GAO. Applications of CT in the Early Prediction of Different Clinical Types of COVID -19 Pneumonia. Journal of Kunming Medical University, 2021, 42(5): 131-137. doi: 10.12259/j.issn.2095-610X.S20210524
[8]	Liao Jing , Xu Chang Jun , Sun Chun Yi , Pan Jing , Zhou Hong Lin , Liu Yang . . Journal of Kunming Medical University, 2020, 41(09): 17-20.
[9]	Ting-ting YU, Xiao-fei LI, Hong-ying WANG, Run-wu ZHANG, Cai-mei DING, Li-hua LI, Jing BAI, Dong-ling LI, Dong PU. Application of Viral Load Testing in Diagnosing 102 Cases of Indeterminate HIV Antibody. Journal of Kunming Medical University, 2020, 41(11): 130-133. doi: 10.12259/j.issn.2095-610X.S20201126
[10]	Zhang Bing Jie , Zhao Wei Zhong , Gao Jing Xia , Zhu Wen Yong , Li Wei Dong , Liao Guo Yang . . Journal of Kunming Medical University, 2020, 41(09): 7-11.
[11]	Yuan Yu , Li Xiang . . Journal of Kunming Medical University, 2020, 41(04): 1-5.
[12]	Gu Li Ming , Zeng Yong , Chen Jun Hui , Wang Fu Ping , Hao Ying Lu , Wu Hai Ying , Ruan Ji Yin , Li Yan Jiao , Chen Yun , Xia Jing , Sun Jia , Hu Juan Yuan , Li Wei , Yu Cong Tao , Ma Chao Xia , Qian Chuan Yun , Hu Min . . Journal of Kunming Medical University, 2020, 41(03): 96-100.
[13]	Ye Wen Jun , Wang Rui Hang , Zeng Xiao Ju , Liu Wei Qing , Liu Fang . . Journal of Kunming Medical University, 2020, 41(02): 137-140.
[14]	Ma Zhi Qiang , Wang Lin , Luo Yu , Qu Chun Yan , Li Bao Ping , Ma Yun Shan , Dong Rui , Wu Yan Rong , Zhao Jin Hua , Xu Ya Lin . . Journal of Kunming Medical University, 2020, 41(03): 128-131.
[15]	Zhu Ya Guang , Liu Jing Song , Li Xi , Hu Yuan , Huang Tian Long , Xiao Zhi Yi . . Journal of Kunming Medical University, 2018, 39(10): 63-67.
[16]	Tang Rui Zhu , Luo Zheng Qiong , Xu Qiu Yue , Zhao Ying , Li Huan , Wang Yun Juan , Song Yu , Liu Zi Jie . The Application of Loop-mediated Amplification Chip in Gene Testing of Common Lower Respiratory Pathogens. Journal of Kunming Medical University, 2017, 38(01): 8-12.
[17]	Bai Zhi Yao . . Journal of Kunming Medical University,
[18]	Li Xiao Fei . . Journal of Kunming Medical University,
[19]	He Ying Hong . . Journal of Kunming Medical University,
[20]	Song Xin . . Journal of Kunming Medical University,