The Expression of C/EBPδ in Colorectal Cancer and Its Effect on the Proliferation of Cancer Cells

Jing-hua LIU; Wei CHANG; Fu-bing YU; Juan SHENG; FENG Cheng-cheng; Ni-shan ZHAO

doi:10.12259/j.issn.2095-610X.S20210913

Volume 42 Issue 9

Sep. 2021

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Jing-hua LIU, Wei CHANG, Fu-bing YU, Juan SHENG, FENG Cheng-cheng, Ni-shan ZHAO. The Expression of C/EBPδ in Colorectal Cancer and Its Effect on the Proliferation of Cancer Cells[J]. Journal of Kunming Medical University, 2021, 42(9): 57-64. doi: 10.12259/j.issn.2095-610X.S20210913

Citation:

Jing-hua LIU, Wei CHANG, Fu-bing YU, Juan SHENG, FENG Cheng-cheng, Ni-shan ZHAO. The Expression of C/EBPδ in Colorectal Cancer and Its Effect on the Proliferation of Cancer Cells[J]. Journal of Kunming Medical University, 2021, 42(9): 57-64. doi: 10.12259/j.issn.2095-610X.S20210913

Citation:

Jing-hua LIU, Wei CHANG, Fu-bing YU, Juan SHENG, FENG Cheng-cheng, Ni-shan ZHAO. The Expression of C/EBPδ in Colorectal Cancer and Its Effect on the Proliferation of Cancer Cells[J]. Journal of Kunming Medical University, 2021, 42(9): 57-64. doi: 10.12259/j.issn.2095-610X.S20210913

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The Expression of C/EBPδ in Colorectal Cancer and Its Effect on the Proliferation of Cancer Cells

doi: 10.12259/j.issn.2095-610X.S20210913

1.
Dept. of Gastroenterology，The Affiliated Hospital of Yunnan University， Kunming Yunnan 650021
2.
School of Public Health，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2021-07-16
Available Online: 2021-09-09
Publish Date: 2021-09-30

Abstract

Abstract

Objective To investigate the expression of C/EBPδ in colorectal cancer and its effect on the proliferation of colorectal cancer cells. Methods The expression of C/EBPδ protein in 40 patients undergoing the radical resection of colon cancer in the Second People’ s Hospital of Yunnan province from 2017 to 2018 was detected by immunohistochemistry. The interaction between C/EBPδ and Siah2 in colorectal cancer tissues was detected by immunoprecipitation. Viral vectors with C/EBPδ overexpression and interference were constructed to infect colorectal cancer caco-2 cells, respectively. The effects of C/EBPδ expression and interference on cell proliferation, apoptosis and cycle were observed through CCK8, flow cytometry detection and plate cloning formation experiments. Chi-square test was used to analyze the correlation factors of positive expression rate. Results Ⅰ The expression of C/EBPδ increased successively from the normal intestinal tissues to paracellular tissues and then to colorectal cancer tissues, and the differences were statistically significant （all P < 0.05）. The expression of C/EBPδ protein was more significant in colorectal cancer patients with liver metastasis; Ⅱ The C/EBPδ increased colorectal cancer tissues were accompanied by the increase of Saih2, and there was an interaction between the two; Ⅲ C/EBPδ overexpression significantly increased the proliferation of caco-2 cells, while the shRNA interference of C/EBP significantly reduced the proliferation of colorectal cancer cells; Ⅳ Compared with the control group, the shRNA interference of C/EBPδ significantly inhibited the in vitro migration and invasion of colorectal cancer cells caco-2. Conclusion C/EBPδ is abnormal in colorectal cancer tissues, and the overexpressed C/EBPδ promotes the invasion and rotation of colorectal cancer cells by interacting with Saih2, which may be related to the poor prognosis of colorectal cancer.
- CCAAT enhancer binding protein,
- Siah2,
- Colorectal cancer,
- Liver metastasis

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