The GalR2 Mediated Analgesic Effect of Needle Knife in Nucleus Accumbens of Rats with Neuropathic Pain

Ya-nan LIU; Shuang YANG; Wei DONG; Chong-yang LI; Shi-lian XU

doi:10.12259/j.issn.2095-610X.S20210914

Volume 42 Issue 9

Sep. 2021

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Ya-nan LIU, Shuang YANG, Wei DONG, Chong-yang LI, Shi-lian XU. The GalR2 Mediated Analgesic Effect of Needle Knife in Nucleus Accumbens of Rats with Neuropathic Pain[J]. Journal of Kunming Medical University, 2021, 42(9): 7-12. doi: 10.12259/j.issn.2095-610X.S20210914

Citation:

Ya-nan LIU, Shuang YANG, Wei DONG, Chong-yang LI, Shi-lian XU. The GalR2 Mediated Analgesic Effect of Needle Knife in Nucleus Accumbens of Rats with Neuropathic Pain[J]. Journal of Kunming Medical University, 2021, 42(9): 7-12. doi: 10.12259/j.issn.2095-610X.S20210914

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The GalR2 Mediated Analgesic Effect of Needle Knife in Nucleus Accumbens of Rats with Neuropathic Pain

doi: 10.12259/j.issn.2095-610X.S20210914

1.
Dept. of Physiology，School of Basic Medicine，Kunming Medical University，Kunming Yunnan 650500
2.
Dept. of Orthopedics，The 3rd Affiliated Hospital of Yunnan Traditional Chinese Medical College，Kunming Yunnan 650041
3.
Dept. of Oncology，The 2nd People’s Hospital of Yunnan Province，Kunming Yunnan 650021，China

Received Date: 2021-07-22
Available Online: 2021-09-13
Publish Date: 2021-09-30

Abstract

Abstract

Objective To investigate the mechanism of galanin receptor 2 （GalR2） in nucleus accumbens （NAc） mediated needle knife intervention in neuralgia. Methods A mononeuropathy model was duplicated by chronic constriction injury （CCI） of the left sciatic nerve of rat. The hind paw withdraw latency （HWL） to noxious thermal stimulation and the hind paw withdraw threshold （HWT） to noxious mechanical stimulation were tested. Western blot was used to detect the effect of needle knife therapy on the expression of GalR2 in NAc of CCI rats. Finally, the HWL and HWT were tested by intra-NAc injection of galanin receptor blockers after the needle knife therapy in CCI rats. Results The increases of HWL （left: P < 0.001; right: P < 0.05）and HWT （left: P < 0.01; right: P < 0.05）induced by the needle knife therapy were reduced by intra-NAc injection of 2 nmol galanin receptors blocker galantide in CCI rats, but not 1 nmol galantide （P > 0.05）. The expression of GalR2 in NAc of CCI rats was upregulated by the needle knife therapy （P < 0.01）, and intra-NAc injections of 2 nmol of GalR2 specific blocker M871 weakened the increase of HWL （left: P < 0.001; right: P < 0.001）and HWT （left: P < 0.001; right: P < 0.01）induced by the needle knife therapy in CCI rats. Conclusion GalR2 may mediate the analgesic effect of the needle knife therapy in NAc of CCI rats.
- GalR2,
- Needle knife,
- Neuropathic pain,
- Analgesic effect

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References(23)

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