Volume 42 Issue 10
Oct.  2021
Turn off MathJax
Article Contents
Abstract
References
Relative Articles
Article Navigation >  Journal of Kunming Medical University  > 2021  >  42(10): 14-21
Keyan WANG, Bingting LIN, Xiaolin WENG, Bin HUANG, Lie WANG. Effectiveness and Safety Analysis of Cyclopol Combined with Remifentanil in Anesthesia for Endoscopic Gastric Cancer Radical Surgery[J]. Journal of Kunming Medical University, 2024, 45(6): 154-160. doi: 10.12259/j.issn.2095-610X.S20240621
Citation: Jun-jie LI, Hai-yan JIANG, Wen-ya BAI, Si-ying HUO, Zhi-sheng SUN, Jian-lin SHAO. Effects of Silencing RND3 Expression on the Inflammatory Response and Apoptosis of Hippocampal Neurons Injured by Oxygen Glucose Deprivation/Reoxygenation[J]. Journal of Kunming Medical University, 2021, 42(10): 14-21. doi: 10.12259/j.issn.2095-610X.S20211012
shu

Effects of Silencing RND3 Expression on the Inflammatory Response and Apoptosis of Hippocampal Neurons Injured by Oxygen Glucose Deprivation/Reoxygenation

doi: 10.12259/j.issn.2095-610X.S20211012
  • 1.

    Dept. of Anesthesiology, The 1st Affiliated Hospital of Kunming Medical University, Kunming Yunnan 650032

  • 2.

    Dept. of Anesthesiology, Mengzi People’s Hospital, Mengzi Yunnan 661100, China

  • Received Date: 2021-07-26
    Available Online: 2021-10-29
  • Publish Date: 2021-10-30
    Abstract
  •   Objective  To investigate the protective effect of silencing Rho family GTPase3 （RND3） expression against oxygen glucose deprivation/reoxygenation （OGD/R） injury in hippocampal neurons via inhibiting inflammation and apoptosis and its mechanism.   Methods  The OGD/R model was established after transfecting cells with lentiviral vectors containing the short hairpin RNA of silencing RND3 gene expression （shRND3） and its negative control （shRND3-NC） sequence. CCK-8 assay was used to detect cell viability. RT-qPCR was used to detect the gene expression of RND3 and inflammation-related and apoptotic molecules. ELISA was used to detect the protein concentration of inflammatory factors in the supernatant of cell culture. Western blot was used to detect the protein expression of RND3, apoptotic proteins and NF-κB of cell nucleus.Cell apoptosis rate was determined by Annexin V-FITC/PI staining and flow cytometry.   Results  Compared with normal cells, the cell viability was decreased after OGD/R injury, the expression of inflammatory factors and NF-κB was increased, the expression of pro-apoptotic proteins was increased, the expression of anti-apoptotic proteins was decreased, and the apoptosis rate of cells was increased. While compared with the negative RND3 silencing group, the cell viability was increased, the expression of inflammatory factors and NF-κB were decreased, the expression of pro-apoptotic protein was decreased, the expression of anti-apoptotic protein was increased, and the apoptosis rate of cells was decreased.   Conclusion  Silencing RND3 alleviates OGD/R injury in hippocampal neurons via inhibiting inflammatory response and apoptosis and the mechanism of inhibiting the inflammatory response by silencing RND3 may be related to the inhibition of NF-κB pathway.
    • FullText(HTML)
    • References(23)
  • [1]
    Goebel U, Scheid S, Spassov S, et al. Argon reduces microglial activation and inflammatory cytokine expression in retinal ischemia/reperfusion injury[J]. Neural Regen Res,2021,16(1):192-198. doi: 10.4103/1673-5374.290098
    [2]
    Chai Z, Gong J, Zheng P, et al. Inhibition of miR-19a-3p decreases cerebral ischemia/reperfusion injury by targeting IGFBP3 in vivo and in vitro[J]. Biol Res,2020,53(1):17. doi: 10.1186/s40659-020-00280-9
    [3]
    Tian Y, Zhong D, Liu Q, et al. Upregulation of miR-216a exerts neuroprotective effects against ischemic injury through negatively regulating JAK2/STAT3-involved apoptosis and inflammatory pathways[J]. J Neurosurg,2018,130(16):977-988.
    [4]
    Fang R, Zhao N, Zeng K, et al. MicroRNA-544 inhibits inflammatory response and cell apoptosis after cerebral ischemia reperfusion by targeting IRAK4[J]. Eur Rev Med Pharmacol Sci,2018,22(17):5605-5613.
    [5]
    Hao M, Xie L, Leng W, et al. Trim47 is a critical regulator of cerebral ischemia-reperfusion injury through regulating apoptosis and inflammation[J]. Biochem Biophys Res Commun,2019,515(4):651-657. doi: 10.1016/j.bbrc.2019.05.065
    [6]
    Ballester-Lurbe B, Poch E, Mocholí E, et al. RhoE is spatiotemporally regulated in the postnatal mouse CNS[J]. Neuroscience,2009,163(2):586-593. doi: 10.1016/j.neuroscience.2009.06.062
    [7]
    Jie W, Andrade K, Lin X, et al. Pathophysiological Functions of Rnd3/RhoE[J]. Compr Physiol,2015,6(1):169-186.
    [8]
    Guasch R, Blanco A, Pérez-Aragó A, et al. RhoE participates in the stimulation of the inflammatory response induced by ethanol in astrocytes[J]. Exp Cell Res,2007,313(17):3779-3788. doi: 10.1016/j.yexcr.2007.07.018
    [9]
    Xu F, Lv C, Deng Y, et al. Icariside II, a PDE5 Inhibitor, suppresses oxygen-glucose deprivation/reperfusion-induced primary hippocampal neuronal death through activating the PKG/CREB/BDNF/TrkB signaling pathway[J]. Front Pharmacol,2020,11:523. doi: 10.3389/fphar.2020.00523
    [10]
    Huang W, Liu X, Cao J, et al. miR-134 regulates ischemia/reperfusion injury-induced neuronal cell death by regulating CREB signaling[J]. J Mol Neurosci,2015,55(4):821-829. doi: 10.1007/s12031-014-0434-0
    [11]
    Leng J, Liu W, Li L, et al. MicroRNA-429/Cxcl1 Axis Protective Against Oxygen Glucose Deprivation/Reoxygenation-Induced Injury in Brain Microvascular Endothelial Cells[J]. Dose Response,2020,18(2):710604679.
    [12]
    Zhang Z, Liu S, Huang S. Thymosin β4 prevents oxygen-glucose deprivation/reperfusion-induced injury in rat cortical neurons[J]. Neuropsychiatr Dis Treat,2019,15:2385-2393. doi: 10.2147/NDT.S208600
    [13]
    Rao G, Zhang W, Song S. MicroRNA-217 inhibition relieves cerebral ischemia/reperfusion injury by targeting SIRT1[J]. Mol Med Rep,2019,20(2):1221-1229.
    [14]
    Liu A, Zhang W, Wang S, et al. HMGB-1/RAGE signaling inhibition by dioscin attenuates hippocampal neuron damage induced by oxygen-glucose deprivation/reperfusion[J]. Exp Ther Med,2020,20(6):231.
    [15]
    Yang Y, Hu F, Yang G, et al. Lack of sphingomyelin synthase 2 reduces cerebral ischemia/reperfusion injury by inhibiting microglial inflammation in mice[J]. Exp Ther Med,2020,20(6):241.
    [16]
    Li Y, Shen R, Wen G, et al. Effects of ketamine on levels of inflammatory cytokines IL-6, IL-1β, and TNF-α in the hippocampus of mice following acute or chronic administration[J]. Front Pharmacol,2017,8:139.
    [17]
    Zhou J, Zhou Q, Zhang T, et al. C7ORF41 Regulates Inflammation by Inhibiting NF-κB Signaling Pathway[J]. Biomed Res Int,2021,2021:7413605.
    [18]
    Moser B, Hochreiter B, Basílio J, et al. The inflammatory kinase IKKα phosphorylates and stabilizes c-Myc and enhances its activity[J]. Mol Cancer,2021,20(1):16. doi: 10.1186/s12943-021-01308-8
    [19]
    Wang Q, Zhou X, Yang L, et al. Gentiopicroside (GENT) protects against sepsis induced by lipopolysaccharide (LPS) through the NF-κB signaling pathway[J]. Ann Transl Med,2019,7(23):731. doi: 10.21037/atm.2019.11.126
    [20]
    Niu Y, Zhang W, Wu P, et al. Expression of the apoptosis-related proteins caspase-3 and NF-kappaB in the hippocampus of Tg2576 mice[J]. Neurosci Bull,2010,26(1):37-46. doi: 10.1007/s12264-010-6122-3
    [21]
    Jiang D, Sun X, Wang S, et al. Upregulation of miR-874-3p decreases cerebral ischemia/reperfusion injury by directly targeting BMF and BCL2L13[J]. Biomed Pharmacother,2019,117:108941. doi: 10.1016/j.biopha.2019.108941
    [22]
    Ma X, Li X, Tian F, et al. Upregulation of RND3 Affects Trophoblast Proliferation, Apoptosis, and Migration at the Maternal-Fetal Interface[J]. Front Cell Dev Biol,2020,8:153. doi: 10.3389/fcell.2020.00153
    [23]
    Dong H, Sun Q, Zhang Y, et al. Genetic deletion of Rnd3 suppresses apoptosis through NF-κB signaling in the brain[J]. Oncol Rep,2021,45(2):595-605.
    • Relative Articles(20)

  • Relative Articles

    [1] Xiaojuan ZHOU, Libin YANG, Peng YANG, Hao GONG, Xu YANG. Impact of Minimally Invasive Extracorporeal Circulation on Systemic Inflammatory Response and Transfusion Requirements after Coronary Artery Bypass Grafting. Journal of Kunming Medical University, 2025, 46(3): 1-8.
    [2] Zhuohui LIU, Shiyin QIN, Hexiang ZHAO, Fengfeng JIA, Biao RUAN, Ruiqing LONG. Inhibitory Effect of Crocin on Pituitary Adenomas via IRF7/NF-κB Signaling Pathway. Journal of Kunming Medical University, 2024, 45(12): 19-27.  doi: 10.12259/j.issn.2095-610X.S20241203
    [3] Yongjun DENG, Qian CHEN, Jianbin ZOU, Zheng GONG, Huanpeng LIU. Overexpression of ZIC1 Gene Inhibits Proliferation of Pleural Mesothelioma Cells by Activating P53 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(4): 35-40.  doi: 10.12259/j.issn.2095-610X.S20240405
    [4] Man YANG, Xingan ZHAO, Yunna GE, Juan QIN, Xiya WANG, Siming TAO. Identification of Atrial Fibrillation-related Inflammatory Genes and Their Association with Immune Cell Infiltration Based on Comprehensive Bioinformatic Analysis. Journal of Kunming Medical University, 2024, 45(3): 18-29.  doi: 10.12259/j.issn.2095-610X.S20240303
    [5] Yidan FU, Wenting CHEN, Xiaoyang SU, Yan ZHAO, Danfeng LAN, Qiuping YANG. Role of Mertk-mediated NF-κb Pathway in Inflammatory Response of Schwann Cells. Journal of Kunming Medical University, 2023, 44(12): 20-24.  doi: 10.12259/j.issn.2095-610X.S20231203
    [6] Yaling ZHAO, Kun WU, Kai WANG, Rong HUANG, Genya HE, Yuhui NA, Miao HE, Zhenbo DING, Caiying ZHANG. Influence of Intrauterine Hyperglycemia Environment on Peripheral Inflammatory Response in Offspring of Gestational Diabetes Mellitus. Journal of Kunming Medical University, 2023, 44(11): 70-75.  doi: 10.12259/j.issn.2095-610X.S20231110
    [7] Guo-yi LIU, Qing-qing ZHAO, Yan WU, Shan-shan LI, Lian-mei ZHONG, Jia GENG. Effect and Mechanism of Puerarin on Experimental Autoimmune Encephalomyelitis in Mice. Journal of Kunming Medical University, 2021, 42(4): 1-7.  doi: 10.12259/j.issn.2095-610X.S20210401
    [8] Li-yuan AN, Bing LI, Gang SU. Effects of Different Doses of Ulinastatinon Postoperative Cognitive Function and HMGB1 in Elderly Patients. Journal of Kunming Medical University, 2021, 42(7): 121-125.  doi: 10.12259/j.issn.2095-610X.S20210720
    [9] Wang Guang , Yang Tong Xin , Jiang Yong Ming , Fang Ke Wei , Liu Jian He , Li Jiong Ming . . Journal of Kunming Medical University, 2019, 40(06): 23-27.
    [10] Li Fei . Effect of Celebrex on the Hemorheology and Levels of Inflammatory Factors in Patients with Delayed Fracture Healing. Journal of Kunming Medical University,
    [11] Yuan Yong . . Journal of Kunming Medical University,
    [12] Li Yan . . Journal of Kunming Medical University,
    [13] Huang Yun Yan . . Journal of Kunming Medical University,
    [14] Zhang Jian . . Journal of Kunming Medical University,
    [15] Pan Yan Li . . Journal of Kunming Medical University,
    [16] Sun Hai Mei . Effects of Ischemic Postconditioning on Myocardial Apoptosis in Non-infarct Zone in Pigs with Myocardium. Journal of Kunming Medical University,
    [17] Miao Yu Lan . . Journal of Kunming Medical University,
    [18] Li Kun Lun . Effect of Survivin Gene Expression Inhibition by RNAi on Breast Cancer SKBr-3 Cells. Journal of Kunming Medical University,
    [19] Zhao Xue Ying . . Journal of Kunming Medical University,
    [20] Li Guo Dong . . Journal of Kunming Medical University,
    • Supplements(0)
    • Cited By

  • Cited by

    Periodical cited type(2)

    1. 张娅，谈玲玲. 瑞芬太尼联合环泊酚对腹腔镜子宫肌瘤剔除术苏醒质量及认知功能的影响. 吉林医学. 2025(02): 434-437 .
    2. 田芳芳，喻倩，李小雅，金淑敏，庄莹莹. 环泊酚在机器人辅助腹腔镜下根治性前列腺癌切除术中的应用. 机器人外科学杂志(中英文). 2025(02): 256-259 .

    Other cited types(0)

  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-040255075100
    Created with Highcharts 5.0.7Chart context menuAccess Class DistributionFULLTEXT: 43.4 %FULLTEXT: 43.4 %META: 56.0 %META: 56.0 %PDF: 0.6 %PDF: 0.6 %FULLTEXTMETAPDF
    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 4.5 %其他: 4.5 %其他: 0.1 %其他: 0.1 %China: 0.2 %China: 0.2 %Falkenstein: 0.3 %Falkenstein: 0.3 %Rochester: 0.1 %Rochester: 0.1 %上海: 14.6 %上海: 14.6 %东莞: 0.4 %东莞: 0.4 %中爪哇省: 0.2 %中爪哇省: 0.2 %保定: 0.2 %保定: 0.2 %六安: 0.2 %六安: 0.2 %北京: 23.3 %北京: 23.3 %十堰: 0.2 %十堰: 0.2 %南通: 0.2 %南通: 0.2 %南阳: 0.1 %南阳: 0.1 %博阿努瓦: 0.1 %博阿努瓦: 0.1 %台北: 0.1 %台北: 0.1 %呼和浩特: 0.1 %呼和浩特: 0.1 %咸阳: 0.1 %咸阳: 0.1 %哥伦布: 0.1 %哥伦布: 0.1 %嘉兴: 0.2 %嘉兴: 0.2 %墨尔本: 0.2 %墨尔本: 0.2 %大同: 0.1 %大同: 0.1 %大连: 0.1 %大连: 0.1 %天津: 0.7 %天津: 0.7 %太原: 0.1 %太原: 0.1 %安那罕: 1.1 %安那罕: 1.1 %安阳: 0.2 %安阳: 0.2 %宣城: 0.1 %宣城: 0.1 %宿迁: 0.1 %宿迁: 0.1 %常州: 0.2 %常州: 0.2 %广安: 0.1 %广安: 0.1 %广州: 0.1 %广州: 0.1 %庆阳: 0.1 %庆阳: 0.1 %张家口: 0.4 %张家口: 0.4 %德罕: 0.2 %德罕: 0.2 %悉尼: 0.1 %悉尼: 0.1 %惠州: 0.1 %惠州: 0.1 %成都: 0.3 %成都: 0.3 %扬州: 0.3 %扬州: 0.3 %拜仁: 0.1 %拜仁: 0.1 %无锡: 0.7 %无锡: 0.7 %日照: 0.1 %日照: 0.1 %昆明: 0.4 %昆明: 0.4 %晋城: 0.1 %晋城: 0.1 %朝阳: 0.2 %朝阳: 0.2 %杭州: 0.5 %杭州: 0.5 %梅州: 0.2 %梅州: 0.2 %武汉: 0.2 %武汉: 0.2 %沈阳: 0.2 %沈阳: 0.2 %沧州: 0.1 %沧州: 0.1 %洛阳: 0.4 %洛阳: 0.4 %济南: 0.1 %济南: 0.1 %淄博: 0.1 %淄博: 0.1 %淮南: 0.1 %淮南: 0.1 %深圳: 0.5 %深圳: 0.5 %温州: 0.3 %温州: 0.3 %湖州: 0.3 %湖州: 0.3 %漯河: 2.2 %漯河: 2.2 %濮阳: 0.1 %濮阳: 0.1 %石家庄: 0.2 %石家庄: 0.2 %芒廷维尤: 14.3 %芒廷维尤: 14.3 %芝加哥: 0.7 %芝加哥: 0.7 %苏州: 0.2 %苏州: 0.2 %西宁: 11.0 %西宁: 11.0 %西安: 0.1 %西安: 0.1 %西雅图: 0.1 %西雅图: 0.1 %许昌: 0.2 %许昌: 0.2 %贵阳: 0.1 %贵阳: 0.1 %资阳: 0.1 %资阳: 0.1 %运城: 0.2 %运城: 0.2 %邯郸: 0.1 %邯郸: 0.1 %郑州: 0.7 %郑州: 0.7 %重庆: 0.4 %重庆: 0.4 %银川: 0.1 %银川: 0.1 %长沙: 1.5 %长沙: 1.5 %长治: 0.1 %长治: 0.1 %青岛: 0.5 %青岛: 0.5 %马赛: 0.2 %马赛: 0.2 %马鞍山: 0.1 %马鞍山: 0.1 %驻马店: 13.8 %驻马店: 13.8 %鹤壁: 0.1 %鹤壁: 0.1 %齐齐哈尔: 0.2 %齐齐哈尔: 0.2 %其他其他ChinaFalkensteinRochester上海东莞中爪哇省保定六安北京十堰南通南阳博阿努瓦台北呼和浩特咸阳哥伦布嘉兴墨尔本大同大连天津太原安那罕安阳宣城宿迁常州广安广州庆阳张家口德罕悉尼惠州成都扬州拜仁无锡日照昆明晋城朝阳杭州梅州武汉沈阳沧州洛阳济南淄博淮南深圳温州湖州漯河濮阳石家庄芒廷维尤芝加哥苏州西宁西安西雅图许昌贵阳资阳运城邯郸郑州重庆银川长沙长治青岛马赛马鞍山驻马店鹤壁齐齐哈尔

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(6)  / Tables(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (3522) PDF downloads(16) Cited by(2)
    Proportional views
    Related

    Copyright © 《昆明医科大学学报》编辑部  滇ICP备00000000号-0

    Publishing: 昆明医科大学学报编辑部

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return