Expression and Significance of Plasma miR-1181 in Patients with Type 2 Diabetes Mellitus

Jing-mei LUO; Can-hui LI; Lan LI; Jie LIN; Jin-rong ZHANG; Li-hua ZHANG

doi:10.12259/j.issn.2095-610X.S20211019

Volume 42 Issue 10

Oct. 2021

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Jing-mei LUO, Can-hui LI, Lan LI, Jie LIN, Jin-rong ZHANG, Li-hua ZHANG. Expression and Significance of Plasma miR-1181 in Patients with Type 2 Diabetes Mellitus[J]. Journal of Kunming Medical University, 2021, 42(10): 61-67. doi: 10.12259/j.issn.2095-610X.S20211019

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Expression and Significance of Plasma miR-1181 in Patients with Type 2 Diabetes Mellitus

doi: 10.12259/j.issn.2095-610X.S20211019

1.
Dept. of General Medicine
2.
Dept. of Diabetes，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2021-07-25
Available Online: 2021-10-29
Publish Date: 2021-10-30

Abstract

Abstract

Objective To verify that miRNA can be used as biomarkers to predict the occurrence and development of diabetes mellitus （DM）, and to explore the expression of miR-1181 in patients with type 2 diabetes mellitus （Type 2 Diabetes Mellitus）. Methods Agilent miRNA chip was used to screen plasma samples from patients with T2DM （n = 5） and normal control group （n = 5）. The differentially expressed miRNA were verified by fluorescence quantitative PCR （qRT-PCR）. The target genes were predicted by bioinformatics methods, and the relationship network diagram of miRNA - target gene - metabolic pathway was drawn. The differentially expressed miRNA: miR-1181, MAP2K2 and MAPK12 in plasma were detected by expanding samples. Results miRNA chip screening and qRT-PCR verified the differentially expressed miRNA associated with T2DM: miR-1181.Bioinformatics analysis showed that the target genes of miR-1181 were CCND1, PI3KR2, MAP2K2 and MAPK12. The expression of miR-1181 in the plasma of T2DM was significantly decreased （P < 0.001）. While expression of MAPK12 mRNA was increased （P < 0.01）. Conclusion The decrease of plasma miR-1181 in T2DM patients may be related to inhibiting effect of target gene MAPK12 on MAPK pathway.
- miR-1181,
- Type 2 Diabetes,
- Target Genes,
- MAPK12

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References(15)

References

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