Volume 42 Issue 10
Oct.  2021
Turn off MathJax
Article Contents
Abstract
References
Relative Articles
Article Navigation >  Journal of Kunming Medical University  > 2021  >  42(10): 61-67
Jing-mei LUO, Can-hui LI, Lan LI, Jie LIN, Jin-rong ZHANG, Li-hua ZHANG. Expression and Significance of Plasma miR-1181 in Patients with Type 2 Diabetes Mellitus[J]. Journal of Kunming Medical University, 2021, 42(10): 61-67. doi: 10.12259/j.issn.2095-610X.S20211019
Citation: Jing-mei LUO, Can-hui LI, Lan LI, Jie LIN, Jin-rong ZHANG, Li-hua ZHANG. Expression and Significance of Plasma miR-1181 in Patients with Type 2 Diabetes Mellitus[J]. Journal of Kunming Medical University, 2021, 42(10): 61-67. doi: 10.12259/j.issn.2095-610X.S20211019
shu

Expression and Significance of Plasma miR-1181 in Patients with Type 2 Diabetes Mellitus

doi: 10.12259/j.issn.2095-610X.S20211019
  • 1.

    Dept. of General Medicine

  • 2.

    Dept. of Diabetes，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

  • Received Date: 2021-07-25
    Available Online: 2021-10-29
  • Publish Date: 2021-10-30
    Abstract
  •   Objective  To verify that miRNA can be used as biomarkers to predict the occurrence and development of diabetes mellitus （DM）, and to explore the expression of miR-1181 in patients with type 2 diabetes mellitus （Type 2 Diabetes Mellitus）.   Methods  Agilent miRNA chip was used to screen plasma samples from patients with T2DM （n = 5） and normal control group （n = 5）. The differentially expressed miRNA were verified by fluorescence quantitative PCR （qRT-PCR）. The target genes were predicted by bioinformatics methods, and the relationship network diagram of miRNA - target gene - metabolic pathway was drawn. The differentially expressed miRNA: miR-1181, MAP2K2 and MAPK12 in plasma were detected by expanding samples.  Results  miRNA chip screening and qRT-PCR verified the differentially expressed miRNA associated with T2DM: miR-1181.Bioinformatics analysis showed that the target genes of miR-1181 were CCND1, PI3KR2, MAP2K2 and MAPK12. The expression of miR-1181 in the plasma of T2DM was significantly decreased （P < 0.001）. While expression of MAPK12 mRNA was increased （P < 0.01）.  Conclusion  The decrease of plasma miR-1181 in T2DM patients may be related to inhibiting effect of target gene MAPK12 on MAPK pathway.
    • FullText(HTML)
    • References(15)
  • [1]
    叶莺,刘小华,严延生. 2型糖尿病患者血浆microRNA差异表达及生物信息学分析[J]. 中华医学遗传学杂志,2021,38(6):536-540.
    [2]
    Ying S Y,Chang D C,Lin S L. The microRNA[J]. Methods MolBiol,2018,1733(10):1-25.
    [3]
    Zhang N. Protocols for the analysis of microRNA expression,biogenesis,and function in immune cells[J]. Curr Protoc Immunol,2019,126(1):e78.
    [4]
    周建萍,毛振彪,吴信华. 微RNA-9对胃癌细胞内CDX2基因表达的影响[J]. 国际消化病杂志,2018,38(5):314-319. doi: 10.3969/j.issn.1673-534X.2018.05.009
    [5]
    郭青松,王鹏,黄龑,等. 微小RNA-30b对胰腺癌干细胞迁移和侵袭的调控作用[J]. 中华医学杂志,2019,99(38):3019-3023. doi: 10.3760/cma.j.issn.0376-2491.2019.38.011
    [6]
    Hua X,Fan K C. Down-regulation of miR-1181 indicates a dismal prognosis for nasopharyngeal carcinoma and promoted cell proliferation and metastasis by modulating Wnt/beta-catenin signaling[J]. Eur Rev Med Pharmacol Sci,2019,23(3):1077-1086.
    [7]
    Ruan L. Serum miR-1181 and miR-4314 associated with ovarian cancer:MiRNA microarray data analysis for a pilot study[J]. Eur J Obstet Gynecol Reprod Biol,2018,222(1):31-38.
    [8]
    Jiang J. MiR-1181 inhibits stem cell-like phenotypes and suppresses SOX2 and STAT3 in human pancreatic cancer[J]. Cancer Lett,2015,356(2PtB):962-70.
    [9]
    Wang J. miR-1181 inhibits invasion and proliferation via STAT3 in pancreatic cancer[J]. World J Gastroenterol,2017,23(9):1594-1601. doi: 10.3748/wjg.v23.i9.1594
    [10]
    Nam E J. MicroRNA profiling of a CD133(+) spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line[J]. BMC Med Genomics,2012,5(18):1-9.
    [11]
    Ruan L. Serum miR-1181 and miR-4314 associated with ovarian cancer:MiRNA microarray data analysis for a pilot study[J]. Eur J Obstet Gynecol Reprod Biol,2018,222(10):31-38.
    [12]
    王杰. miRNA-1181通过抑制STAT3影响人胰腺癌细胞的侵袭转移能力[J]. 贵州医科大学学报,2016,41(12):1387-1392.
    [13]
    Van Allen EM,Wagle N,Sucker A,et al. The genetic landscape of clinical resistance to RAF inhibition in metastatic melanoma[J]. Cancer Discov,2014,4(1):94-109. doi: 10.1158/2159-8290.CD-13-0617
    [14]
    Zhong W,Zhu H,Sheng F,et al. Activation of the MAPK11/12/13/14 (p38 MAPK) pathway regulates the transcription of autophagy genes in response to oxidative stress induced by a novel copper complex in HeLa cells[J]. Autophagy,2014,10(7):1285-1300. doi: 10.4161/auto.28789
    [15]
    窦艳,邱鹏,王丽. NEK2及ERK2在不同病理类型乳腺癌中的表达及意义[J]. 临床合理用药杂志,2019,12(14):120-121.
    • Relative Articles(20)

  • Relative Articles

    [1] Yu CHANG, Li GUI, Ruonan LI. Observation of Liraglutide's Effect on Function of Pancreatic Islets β-Cell in Type 2 Diabetes. Journal of Kunming Medical University, 2025, 46(2): 134-140.  doi: 10.12259/j.issn.2095-610X.S20250219
    [2] Dan MA, Yajuan ZHANG, Binbin MA, Qiaoning YUE, Jianping LIU. Genomic Analysis of Cellular Senescence and Osteoporosis Target Genes and Small Molecule Screening. Journal of Kunming Medical University, 2025, 46(2): 141-150.  doi: 10.12259/j.issn.2095-610X.S20250220
    [3] Xinlin ZHU, Yanan WU, Qi MENG, Qianzi YANG, Yuanbiao LI, Junheng TAO, Mengyang HE. Influence of Medication Compliance of Type 2 Diabetes Chronic Disease Management Patients in Two Communities of Kunming on Disease Control. Journal of Kunming Medical University, 2024, 45(3): 79-83.  doi: 10.12259/j.issn.2095-610X.S20240312
    [4] Ling NIU, Rong MA, Cheng ZHANG, Cuijuan MIAO, Yan TANG, Fang LIU, Boyi LI. Analysis of PTH and ER Gene Polymorphisms in Patients with Type 2 Diabetes Mellitus Complicated with Osteoporosis. Journal of Kunming Medical University, 2024, 45(6): 106-112.  doi: 10.12259/j.issn.2095-610X.S20240614
    [5] Hong TAN, Hejia YIN, Yan LI, Qin HU, Lan LI, Zundan REN, Rou SHI. Correlation between DDAH1 Gene 836A/T Polymorphism and Type 2 Diabetic Kidney Disease in Yunnan Han Population. Journal of Kunming Medical University, 2023, 44(6): 26-32.  doi: 10.12259/j.issn.2095-610X.S20230621
    [6] Boyi LI, Ling NIU, Rong MA, Xian ZHANG, Fang LIU, Yan TANG, Cuijuan MIAO, Cheng ZHANG, Zhujun HAN. The Relationship Between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis. Journal of Kunming Medical University, 2022, 43(3): 67-73.  doi: 10.12259/j.issn.2095-610X.S20220306
    [7] Jie CHEN, Wei-chang GUO, He-jia YIN, Hong-xia YAN, Fan ZHANG, Yao-yi WANG, Hui-fang LI. The Interaction among Gene Polymorphisms of RANTES and Its Receptor CCR5 and Environmental Factors in the Development of T2DM in Kunming Han Nationality. Journal of Kunming Medical University, 2021, 42(6): 38-44.  doi: 10.12259/j.issn.2095-610X.S20210606
    [8] Bo-yi LI, Ling NIU, Rong MA, Xian ZHANG, Fang LIU, Yan TANG, Cui-juan MIAO, Zhu-jun HAN, Cheng ZHANG. Association between Calcitonin Receptor Gene Polymorphism and Type 2 Diabetes with Osteoporosis in Kunming. Journal of Kunming Medical University, 2021, 42(7): 57-63.  doi: 10.12259/j.issn.2095-610X.S20210709
    [9] Ling NIU, Bo-yi LI, Jing-qiu MAO, Yan TANG, Rong MA, Fang LIU, Chen ZHANG, Zhu-jun HAN, Cui-juan MIAO, Xian ZHANG. The Relationship between Vitamin D Receptor Gene Polymorphism and Type 2 Diabetes Mellitus with Osteoporosis in Kunming Area. Journal of Kunming Medical University, 2021, 42(7): 70-76.  doi: 10.12259/j.issn.2095-610X.S20210711
    [10] Bo-yi LI, Ling NIU, Rong MA, Xian ZHANG, Fang LIU, Yan TANG, Cui-juan MIAO, Zhu-jun HAN, Cheng ZHANG. The Relationship between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis in Kunming Area. Journal of Kunming Medical University, 2021, 43(): 1-8.  doi: 10.12259/j.issn.2095-610X.S20211112
    [11] Cong MEI, Xiao-chun WENG, Bao-kun PENG, Sui-jun YAN, Chun LI, Qiong ZHOU, Zhe TANG. Correlation between rs4580704 Polymorphism of CLOCL Gene and Type 2 Diabetes and Sleep Quality. Journal of Kunming Medical University, 2021, 42(3): 135-139.  doi: 10.12259/j.issn.2095-610X.S20210332
    [12] Ling NIU, Bo-yi LI, Cheng ZHANG, Rong MA, Yan TANG, Fang LIU, Li-min YIN, Zhu-jun HAN, Cui-juan MIAO, Xian ZHANG. Relationship between Calcitonin Receptor，Vitamin D Receptor Gene Polymorphism and Type 2 Diabetes Mellitus with Osteoporosis in Kunming Area. Journal of Kunming Medical University, 2021, 42(11): 74-80.  doi: 10.12259/j.issn.2095-610X.S20211114
    [13] Xiang Xi . The Correlation between Vitamin D Receptor Gene FokI Single Nucleotide Polymorphism and Diabetic Kidney Disease. Journal of Kunming Medical University,
    [14] Nian Xin . Difference of Metallothionein Gene G-201A Polymorphism in Type 2 Diabetes and Essential Hypertension. Journal of Kunming Medical University,
    [15] Du Juan . . Journal of Kunming Medical University,
    [16] Deng De Yao . . Journal of Kunming Medical University,
    [17] Chen Bo . . Journal of Kunming Medical University,
    [18] Li Xian Li . . Journal of Kunming Medical University,
    [19] Wang Yan . . Journal of Kunming Medical University,
    [20] Nian Xin . The Preliminary Analysis of the FOXC2 C-1702T Polymorphism is Associated with Cholesterol. Journal of Kunming Medical University, 2012, 33(02): 1-1.
    • Supplements(0)
    • Cited By

  • Cited by

    Periodical cited type(4)

    1. 朱珊珊，祁荣，杨丽琴. 正向反馈式心理护理对间质性肺炎患者疾病认知及健康行为的改善作用. 心理月刊. 2024(12): 173-175+186 .
    2. 陶梅梅，李菲，胡家欣，朱光发. 具有自身免疫特征的间质性肺炎的临床特点及支气管肺泡灌洗液细胞分类的应用. 国际呼吸杂志. 2024(05): 585-591 .
    3. 岩碧琼. 补肺活血胶囊联合低分子肝素治疗间质性肺炎的效果及对肺功能、运动耐力的影响. 吉林医学. 2023(07): 1884-1887 .
    4. 张明波. 乙酰半胱氨酸联合甲泼尼龙琥珀酸钠治疗特发性间质性肺炎的临床效果. 临床合理用药杂志. 2022(14): 60-62 .

    Other cited types(1)

  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-040255075100125150
    Created with Highcharts 5.0.7Chart context menuAccess Class DistributionFULLTEXT: 21.2 %FULLTEXT: 21.2 %META: 73.6 %META: 73.6 %PDF: 5.2 %PDF: 5.2 %FULLTEXTMETAPDF
    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 11.6 %其他: 11.6 %其他: 1.5 %其他: 1.5 %Falkenstein: 1.3 %Falkenstein: 1.3 %Knoxville: 0.7 %Knoxville: 0.7 %San Jose: 0.6 %San Jose: 0.6 %上海: 1.8 %上海: 1.8 %仁川广域: 0.4 %仁川广域: 0.4 %包头: 0.2 %包头: 0.2 %北京: 0.9 %北京: 0.9 %十堰: 1.3 %十堰: 1.3 %华盛顿州: 0.2 %华盛顿州: 0.2 %南京: 0.4 %南京: 0.4 %南宁: 0.2 %南宁: 0.2 %南通: 0.4 %南通: 0.4 %厦门: 0.4 %厦门: 0.4 %台州: 1.1 %台州: 1.1 %哈尔滨: 0.2 %哈尔滨: 0.2 %哥伦布: 0.9 %哥伦布: 0.9 %嘉兴: 1.1 %嘉兴: 1.1 %墨西哥城: 0.2 %墨西哥城: 0.2 %天津: 2.2 %天津: 2.2 %太原: 0.2 %太原: 0.2 %宁波: 0.4 %宁波: 0.4 %安康: 0.2 %安康: 0.2 %宣城: 1.1 %宣城: 1.1 %常州: 1.1 %常州: 1.1 %平顶山: 0.4 %平顶山: 0.4 %广州: 1.3 %广州: 1.3 %张家口: 4.8 %张家口: 4.8 %张家界: 0.2 %张家界: 0.2 %成都: 0.6 %成都: 0.6 %扬州: 2.8 %扬州: 2.8 %昆明: 2.4 %昆明: 2.4 %朝阳: 0.2 %朝阳: 0.2 %杭州: 2.0 %杭州: 2.0 %武汉: 1.8 %武汉: 1.8 %江门: 0.2 %江门: 0.2 %沈阳: 0.2 %沈阳: 0.2 %淄博: 0.6 %淄博: 0.6 %深圳: 0.2 %深圳: 0.2 %温州: 2.6 %温州: 2.6 %湖州: 0.7 %湖州: 0.7 %湘潭: 0.4 %湘潭: 0.4 %漯河: 12.4 %漯河: 12.4 %玉溪: 4.6 %玉溪: 4.6 %白山: 0.2 %白山: 0.2 %百色: 0.2 %百色: 0.2 %盐城: 0.2 %盐城: 0.2 %石家庄: 1.3 %石家庄: 1.3 %芒廷维尤: 9.4 %芒廷维尤: 9.4 %芝加哥: 0.4 %芝加哥: 0.4 %衡阳: 0.7 %衡阳: 0.7 %襄阳: 0.6 %襄阳: 0.6 %西宁: 10.0 %西宁: 10.0 %西安: 0.9 %西安: 0.9 %诺沃克: 0.7 %诺沃克: 0.7 %赣州: 0.2 %赣州: 0.2 %运城: 0.2 %运城: 0.2 %邯郸: 1.1 %邯郸: 1.1 %郑州: 1.7 %郑州: 1.7 %重庆: 1.3 %重庆: 1.3 %长沙: 1.5 %长沙: 1.5 %青岛: 0.6 %青岛: 0.6 %马鞍山: 0.4 %马鞍山: 0.4 %其他其他FalkensteinKnoxvilleSan Jose上海仁川广域包头北京十堰华盛顿州南京南宁南通厦门台州哈尔滨哥伦布嘉兴墨西哥城天津太原宁波安康宣城常州平顶山广州张家口张家界成都扬州昆明朝阳杭州武汉江门沈阳淄博深圳温州湖州湘潭漯河玉溪白山百色盐城石家庄芒廷维尤芝加哥衡阳襄阳西宁西安诺沃克赣州运城邯郸郑州重庆长沙青岛马鞍山

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(7)  / Tables(3)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (3017) PDF downloads(18) Cited by(5)
    Proportional views
    Related

    Copyright © 《昆明医科大学学报》编辑部  滇ICP备00000000号-0

    Publishing: 昆明医科大学学报编辑部

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return