Expression and Significance of Plasma miR-1181 in Patients with Type 2 Diabetes Mellitus

Jing-mei LUO; Can-hui LI; Lan LI; Jie LIN; Jin-rong ZHANG; Li-hua ZHANG

doi:10.12259/j.issn.2095-610X.S20211019

Volume 42 Issue 10

Oct. 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(10): 61-67

Jing-mei LUO, Can-hui LI, Lan LI, Jie LIN, Jin-rong ZHANG, Li-hua ZHANG. Expression and Significance of Plasma miR-1181 in Patients with Type 2 Diabetes Mellitus[J]. Journal of Kunming Medical University, 2021, 42(10): 61-67. doi: 10.12259/j.issn.2095-610X.S20211019

Citation:

Jing-mei LUO, Can-hui LI, Lan LI, Jie LIN, Jin-rong ZHANG, Li-hua ZHANG. Expression and Significance of Plasma miR-1181 in Patients with Type 2 Diabetes Mellitus[J]. Journal of Kunming Medical University, 2021, 42(10): 61-67. doi: 10.12259/j.issn.2095-610X.S20211019

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Expression and Significance of Plasma miR-1181 in Patients with Type 2 Diabetes Mellitus

doi: 10.12259/j.issn.2095-610X.S20211019

1.
Dept. of General Medicine
2.
Dept. of Diabetes，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2021-07-25
Available Online: 2021-10-29
Publish Date: 2021-10-30

Abstract

Abstract

Objective To verify that miRNA can be used as biomarkers to predict the occurrence and development of diabetes mellitus （DM）, and to explore the expression of miR-1181 in patients with type 2 diabetes mellitus （Type 2 Diabetes Mellitus）. Methods Agilent miRNA chip was used to screen plasma samples from patients with T2DM （n = 5） and normal control group （n = 5）. The differentially expressed miRNA were verified by fluorescence quantitative PCR （qRT-PCR）. The target genes were predicted by bioinformatics methods, and the relationship network diagram of miRNA - target gene - metabolic pathway was drawn. The differentially expressed miRNA: miR-1181, MAP2K2 and MAPK12 in plasma were detected by expanding samples. Results miRNA chip screening and qRT-PCR verified the differentially expressed miRNA associated with T2DM: miR-1181.Bioinformatics analysis showed that the target genes of miR-1181 were CCND1, PI3KR2, MAP2K2 and MAPK12. The expression of miR-1181 in the plasma of T2DM was significantly decreased （P < 0.001）. While expression of MAPK12 mRNA was increased （P < 0.01）. Conclusion The decrease of plasma miR-1181 in T2DM patients may be related to inhibiting effect of target gene MAPK12 on MAPK pathway.
- miR-1181,
- Type 2 Diabetes,
- Target Genes,
- MAPK12

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References(15)

References

[1]	叶莺,刘小华,严延生. 2型糖尿病患者血浆microRNA差异表达及生物信息学分析[J]. 中华医学遗传学杂志,2021,38(6):536-540.
[2]	Ying S Y,Chang D C,Lin S L. The microRNA[J]. Methods MolBiol,2018,1733(10):1-25.
[3]	Zhang N. Protocols for the analysis of microRNA expression,biogenesis,and function in immune cells[J]. Curr Protoc Immunol,2019,126(1):e78.
[4]	周建萍,毛振彪,吴信华. 微RNA-9对胃癌细胞内CDX2基因表达的影响[J]. 国际消化病杂志,2018,38(5):314-319. doi: 10.3969/j.issn.1673-534X.2018.05.009
[5]	郭青松,王鹏,黄龑,等. 微小RNA-30b对胰腺癌干细胞迁移和侵袭的调控作用[J]. 中华医学杂志,2019,99(38):3019-3023. doi: 10.3760/cma.j.issn.0376-2491.2019.38.011
[6]	Hua X,Fan K C. Down-regulation of miR-1181 indicates a dismal prognosis for nasopharyngeal carcinoma and promoted cell proliferation and metastasis by modulating Wnt/beta-catenin signaling[J]. Eur Rev Med Pharmacol Sci,2019,23(3):1077-1086.
[7]	Ruan L. Serum miR-1181 and miR-4314 associated with ovarian cancer:MiRNA microarray data analysis for a pilot study[J]. Eur J Obstet Gynecol Reprod Biol,2018,222(1):31-38.
[8]	Jiang J. MiR-1181 inhibits stem cell-like phenotypes and suppresses SOX2 and STAT3 in human pancreatic cancer[J]. Cancer Lett,2015,356(2PtB):962-70.
[9]	Wang J. miR-1181 inhibits invasion and proliferation via STAT3 in pancreatic cancer[J]. World J Gastroenterol,2017,23(9):1594-1601. doi: 10.3748/wjg.v23.i9.1594
[10]	Nam E J. MicroRNA profiling of a CD133(+) spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line[J]. BMC Med Genomics,2012,5(18):1-9.
[11]	Ruan L. Serum miR-1181 and miR-4314 associated with ovarian cancer:MiRNA microarray data analysis for a pilot study[J]. Eur J Obstet Gynecol Reprod Biol,2018,222(10):31-38.
[12]	王杰. miRNA-1181通过抑制STAT3影响人胰腺癌细胞的侵袭转移能力[J]. 贵州医科大学学报,2016,41(12):1387-1392.
[13]	Van Allen EM,Wagle N,Sucker A,et al. The genetic landscape of clinical resistance to RAF inhibition in metastatic melanoma[J]. Cancer Discov,2014,4(1):94-109. doi: 10.1158/2159-8290.CD-13-0617
[14]	Zhong W,Zhu H,Sheng F,et al. Activation of the MAPK11/12/13/14 (p38 MAPK) pathway regulates the transcription of autophagy genes in response to oxidative stress induced by a novel copper complex in HeLa cells[J]. Autophagy,2014,10(7):1285-1300. doi: 10.4161/auto.28789
[15]	窦艳,邱鹏,王丽. NEK2及ERK2在不同病理类型乳腺癌中的表达及意义[J]. 临床合理用药杂志,2019,12(14):120-121.

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