Analysis of Triterpene Chemical Constituents in <i>Poria cocos</i> 95% Ethanol Extract via UPLC-IT-TOF/MS

Xing-long CHEN; Min ZHANG; Qi-yao YANG; Yong-mou LUO; Ting-ting CAO; Li-ping TANG; Yong LI

doi:10.12259/j.issn.2095-610X.S20211101

Volume 42 Issue 11

Nov. 2021

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Xing-long CHEN, Min ZHANG, Qi-yao YANG, Yong-mou LUO, Ting-ting CAO, Li-ping TANG, Yong LI. Analysis of Triterpene Chemical Constituents in Poria cocos 95% Ethanol Extract via UPLC-IT-TOF/MS[J]. Journal of Kunming Medical University, 2021, 42(11): 1-8. doi: 10.12259/j.issn.2095-610X.S20211101

Citation:

Xing-long CHEN, Min ZHANG, Qi-yao YANG, Yong-mou LUO, Ting-ting CAO, Li-ping TANG, Yong LI. Analysis of Triterpene Chemical Constituents in Poria cocos 95% Ethanol Extract via UPLC-IT-TOF/MS[J]. Journal of Kunming Medical University, 2021, 42(11): 1-8. doi: 10.12259/j.issn.2095-610X.S20211101

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Analysis of Triterpene Chemical Constituents in Poria cocos 95% Ethanol Extract via UPLC-IT-TOF/MS

doi: 10.12259/j.issn.2095-610X.S20211101

1.
College of Traditional Chinese Medicine & Yunnan Key Laboratory of Southern Medicine Utilization，Yunnan University of Chinese Medicine，Kunming Yunnan 650504
2.
Dept. of Ophthalmology，The First Hospital of Kunming，Kunming Yunnan 650224
3.
School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology of Natural Products，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2021-09-25
Available Online: 2021-11-15
Publish Date: 2021-11-30

Abstract

Abstract

Objective To evaluate the triterpene chemical constituents in Poria cocos 95% ethanol extract by ultra-performance liquid chromatography ion trap time-of-flight mass spectrometry （UPLC-IT-TOF/MS） method. Methods UPLC was performed with the UHPLC XB-C18 and methanoic acid/water （0.05/100, v/v）-acetonitrile solution （gradient elution） was employed with a flow rate of 0.2 mL·min^-1. The detection wavelength range was 190～400 nm. Triterpene chemical compositions in Poria cocos were qualitatively analyzed using ESI ion with electrospray positive and negative ions simultaneous detection, sodium trifluoroacetace correction and Shimadzu Composition Formula Predictor software. Results Through the analysis of the multistage tandem mass spectrometry, combined with the application of nitrogen rule and the Scifinder database search, 22 triterpenes with lanostan-8-en, lanostan-7, 9（11）-diene type and other lanostane triterpene were identified. Conclusion This study quickly analyzed the triterpenes in the Poria cocos ethanol extract via LC-MS, which provided the basis for the pharmacodyamic material basis and quality control.
- Poria cocos,
- Triterpene,
- UPLC-IT-TOF/MS

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References(20)

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