Correlation between Changes of Vaginal Microflora and Premature Rupture of Membranes during Pregnancy

Shan-shan GUO; Ji-hong LIU

doi:10.12259/j.issn.2095-610X.S20211107

Volume 42 Issue 11

Nov. 2021

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2021 > 42(11): 38-44

Shan-shan GUO, Ji-hong LIU. Correlation between Changes of Vaginal Microflora and Premature Rupture of Membranes during Pregnancy[J]. Journal of Kunming Medical University, 2021, 42(11): 38-44. doi: 10.12259/j.issn.2095-610X.S20211107

Citation:

Shan-shan GUO, Ji-hong LIU. Correlation between Changes of Vaginal Microflora and Premature Rupture of Membranes during Pregnancy[J]. Journal of Kunming Medical University, 2021, 42(11): 38-44. doi: 10.12259/j.issn.2095-610X.S20211107

Citation:

PDF( 1313 KB)

Correlation between Changes of Vaginal Microflora and Premature Rupture of Membranes during Pregnancy

doi: 10.12259/j.issn.2095-610X.S20211107

Shan-shan GUO,
Ji-hong LIU^,

Dept. of Obstetrics，The 2nd Affiliated Hospital of Kunming Medical University ， Kunming Yunnan 650101，China

Received Date: 2021-10-08
Publish Date: 2021-11-25

Abstract

Abstract

Objective To elucidate the relationship between vaginal microbiota and premature rupture of membranes by analyzing the vaginal microbiota of normal pregnancy and pregnant women of premature rupture of membranes. Methods Gestational age meeting inclusion criteria was randomly collected （gestational age < 28 weeks; 28 to 34 weeks; 34 and 37 weeks; 37 to 39 weeks; each case was 10 at 39-41 weeks） , a total of 50 cases of vaginal secretions from pregnant women with premature rupture of membranes were used as the experimental group. Vaginal secretions were collected from 6 normal pregnant women at the above relative gestational age, and a total of 30 normal women were selected as the control group. The bacterial DNA of vaginal secretions was extracted by the second generation high throughput sequencing method, and the diversity index, microbial community structure and dominant species of vaginal microbiome were analyzed and compared between premature rupture of membranes and normal pregnant women. Results The P value of Wilcoxon test for Alpha diversity richness and evenness index among each group was > 0.05, The Stress value of nonmetric multidimensional scaling was = 0.02, less than 0.05, the difference was statistically significant. According to Venn diagram, the species diversity of preterm premature rupture of membranes was the highest （529）. The diversity of normal control group before term was the lowest （207）. Conclusions In normal pregnancy, vaginal microflora is stable, with small Alpha diversity and significant Beta diversity. Vaginal microbial community during pregnancy can be divided into five types, mainly L. crispatus , if the increase of L.iners, gardnella, prevotella,sneathia , ureaplasma and atopobium etc in the vagina during pregnancy, bacterial vaginosis and premature rupture of membranes are prone to occur.
- Vaginal microbial community,
- Second generation high-throughput sequencing,
- Premature rupture of membranes,
- Lactobacillus

FullText(HTML)

References(23)

References

[1]	Sobel J D. Bacterial vaginosis[J]. Annu Rev Med,2000,51(3):349-356.
[2]	Schwebke J R. Gynecologic consequences of bacterial vaginosis[J]. Obstet Gynecol Clin North Am,2003,30(4):685-694. doi: 10.1016/S0889-8545(03)00086-X
[3]	Romero R,Hassan S S,Gajer P,et al. The composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women[J]. BioMed Central,2014,2(1):32-40.
[4]	Schönfeld A,Feldt T,Tufa T B,et al. Prevalence and impact of sexually transmitted infections in pregnant women in Central Ethiopia[J]. Int J STD AIDS,2018,29(3):251-258. doi: 10.1177/0956462417723545
[5]	于燕,朱元方,夏勇,等. 孕妇及新生儿沙眼衣原体感染的基因型分析[J]. 中华妇产科杂志,2018,53(5):319-322. doi: 10.3760/cma.j.issn.0529-567x.2018.05.007
[6]	Jimenez A,Sanchez A,Rey A,et al. Recovery of aerobic and anaerobic bacteria from patients with acute appendicitis using blood culture bottles[J]. Biomedica,2019,39(4):699-706. doi: 10.7705/biomedica.4774
[7]	Guindo C O,Terrer E,Chabriere E,et al. Culture of salivary methanogens assisted by chemically produced hydrogen[J]. Anaerobe,2020,61(4):102128.
[8]	Caporaso J Gregory,Lauber Christian L,Walters William A,et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample.[J]. Proceedings of the National Academy of Sciences of the United States of America,2011,108(S1):4516-4522.
[9]	MacIntyre David A,Chandiramani Manju,Lee Yun S,et al. The vaginal microbiome during pregnancy and the postpartum period in a European population.[J]. Scientific Reports,2015,5(1):8988. doi: 10.1038/srep08988
[10]	Blaxter Mark,Mann Jenna,Chapman Tom,et al. Defining operational taxonomic units using DNA barcode data[J]. Philosophical Transactions of The Royal Society B,2005,360(1462):1935-1943. doi: 10.1098/rstb.2005.1725
[11]	Liang Xinyuan,Li Zhe,Tye Kian Deng,et al. The effect of probiotic supplementation during pregnancy on the interaction network of vaginal microbiome.[J]. Journal of Obstetrics and Gynaecology Research,2020,47(1):103-113.
[12]	Richard W Hyman,Marilyn Fukushima,Hui Jiang,et al. Diversity of the vaginal microbiome correlates with preterm birth[J]. Reproductive Sciences,2014,21(1):32-40. doi: 10.1177/1933719113488838
[13]	Pawel Gajer,Rebecca M Brotman,Guoyun Bai,et al. Temporal dynamics of the human vaginal microbiota[J]. Science Translational Medicine,2012,4(132):132ra52.
[14]	Buchta V. Vaginal microbiome[J]. Ceska Gynekologie,2018,83(5):371-379.
[15]	Barrientos Dur á n Antonio,Fuentes L ó pez Ana,de Salazar Adolfo,et al. Reviewing the composition of vaginal microbiota:Inclusion of nutrition and probiotic factors in the maintenance of eubiosis[J]. Nutrients,2020,12(2):419. doi: 10.3390/nu12020419
[16]	Spurbeck R R,Arvidson C G. Lactobacilli at the front line of defense against vaginally acquired infections[J]. Future Microbiol,2011,6(5):567-582. doi: 10.2217/fmb.11.36
[17]	Rinku Pramanick,Niranjan Mayadeo,Himangi Warke,et al. Vaginal microbiota of asymptomatic bacterial vaginosis and vulvovaginal candidiasis:Are they different from normal microbiota?[J]. Microbial Pathogenesis,2019,134(2):103599.
[18]	Pendharkar S,Magopane T,Larsson P G,et al. Identification and characterisation of vaginal lactobacilli from South African women.[J]. BMC Infectious Diseases,2013,13(1):43. doi: 10.1186/1471-2334-13-43
[19]	Mario Vaneechoutte. Lactobacillus iners,the unusual suspect[J]. Research in Microbiology,2017,168(9-10):826-836. doi: 10.1016/j.resmic.2017.09.003
[20]	Mariya I Petrova,Gregor Reid,Mario Vaneechoutte,et al. Lactobacillus iners:Friend or foe?[J]. Trends in Microbiology,2017,25(3):182-191. doi: 10.1016/j.tim.2016.11.007
[21]	闻先萍. 结合珠蛋白基因多态性与胎膜早破的相关性研究[D]. 芜湖: 皖南医学院硕士论文, 2015.
[22]	N Tabatabaei,A M Eren,L B Barreiro,et al. Vaginal microbiome in early pregnancy and subsequent risk of spontaneous preterm birth:a case–control study[J]. BJOG:An International Journal of Obstetrics & Gynaecology,2019,126(3):349-358.
[23]	Molly J Stout,Yanjiao Zhou,Kristine M Wylie,et al. Early pregnancy vaginal microbiome trends and preterm birth[J]. American Journal of Obstetrics and Gynecology,2017,217(3):356.e1-356.e18. doi: 10.1016/j.ajog.2017.05.030

Relative Articles(20)

Relative Articles

[1]	Danyan SU, Wentian TANG, Jinxu YANG, Hua LIU, Bangsheng LI, Yingding ZHAO, Yunchao HUANG. Research Progress of Indole in E. coli Biofilms on the Surface of Biomaterials. Journal of Kunming Medical University, 2023, 44(12): 184-190. doi: 10.12259/j.issn.2095-610X.S20231229
[2]	Chun LI, Qiong ZHOU, Cong MEI, Jie jie HUANG, Yi peng WANG, Song lan ZHOU, Qian ZHENG, Zhe TANG. Effect of Bifidobacterium trisporus on Intestinal Microbiota in the Mouse Model of Non-alcoholic Fatty Liver Disease. Journal of Kunming Medical University, 2023, 44(10): 77-82. doi: 10.12259/j.issn.2095-610X.S20231022
[3]	Tong XU, Yantuanjin MA, Yuhang ZHANG, Qiuyue HE, Wei HUANG, Mengxin LU, Jian TANG, Jianping HE, Guoqing JIANG, Yuan QIAN. Second-generation Sequencing Study of Trophoblast Cells in vitro Stimulated by Different Concentrations of Vitamin D. Journal of Kunming Medical University, 2022, 43(10): 1-9. doi: 10.12259/j.issn.2095-610X.S20221021
[4]	Siyuan GAO, Guifang HU, Yiqiong ZHANG, Mingchun WANG, Junyi LI, Jiqun YANG. Environmental Sanitation Improvement on Environmental Microbe and Infections of Inpatients. Journal of Kunming Medical University, 2022, 43(10): 131-135. doi: 10.12259/j.issn.2095-610X.S20221007
[5]	Xian BAI, Yao CUI, Qing ZHOU, Xiaoli LI, Yuexiao LI, Juan LIU, Shinan ZHANG. Salivary Microbial Profiles of Dental Caries among Lisu Preschool Children in Yunnan Province. Journal of Kunming Medical University, 2022, 43(3): 86-93. doi: 10.12259/j.issn.2095-610X.S20220301
[6]	Sainan LI, Wenjin QI, Raoyang KE, Rong HUANG, Guangqiong YANG. The Role of Maternal Serum MMPs and SAA in the Pathogenesis of Preterm Premature Rupture of Membranes. Journal of Kunming Medical University, 2022, 43(9): 29-33. doi: 10.12259/j.issn.2095-610X.S20220905
[7]	Xinyi LI, Yuexiao LI, Lixian LI, Yongping LI, Ying GAO, Yanhong LI. Study on Microbiome of Supragingival Plaque of College Students with Gingivitis. Journal of Kunming Medical University, 2022, 43(2): 102-106. doi: 10.12259/j.issn.2095-610X.S20220207
[8]	Min ZHAO, Hui YANG, Wei-hao MA, Shan-shan LI. Effects of Different Ventilation Modes on PaO₂ and Lactic Acid in Arterial Blood of Upper and Lower Limbs in Rectal Cancer Patients under Trendelenburg Posture. Journal of Kunming Medical University, 2021, 42(4): 61-66. doi: 10.12259/j.issn.2095-610X.S20210412
[9]	Qian ZHAO, Xia HE, Ya LING, Rao-yang KE, Sai-nan LI, Rong HUANG, Wen-jin QI. The Clinical Value of Detection of TNF-α、IL-6 and IL-17 in Premature Ruptureof Membranes with Adverse Pregnancy Outcomes Associated with Maternal and Fetal Infection. Journal of Kunming Medical University, 2021, 42(9): 101-107. doi: 10.12259/j.issn.2095-610X.S20210940
[10]	Li Ling Jie , Su Jing , Gao Hai Xia , Wang Pei Pei , Xi Zuo Ming , Zhang Guang Xia . . Journal of Kunming Medical University, 2020, 41(01): 96-99.
[11]	Yin Shun Hui , Zhan Ye Ming , Jia Feng Mei , Ran Li Quan , Zhang Ming Zhu . . Journal of Kunming Medical University, 2019, 40(04): 23-30.
[12]	Zhang An Xing , Wu Jing , Sun Yang , Miao Ying Lei . . Journal of Kunming Medical University, 2018, 39(09): 128-132.
[13]	Zhou Ying . Clinical Application of the Next-generation Sequencing Technology. Journal of Kunming Medical University,
[14]	Yi Hai Ping . Influence of Candida and Aerobic Bacteria Infection on Maternal and Neonatal Outcomes of Premature Rupture of Membranes. Journal of Kunming Medical University,
[15]	Li Yu Hua . The Relationship between the Bacterial Vaginosis and 8 Putative Periodontal Pathogen Infection. Journal of Kunming Medical University,
[16]	Zhang Wen . The Clinical Analysis of Three Kinds of Treatment Regimens for 300 Cases of Bacterial Vaginal Disease. Journal of Kunming Medical University,
[17]	Li Hui . Correlation Analysis between Prenatal Infection and Premature Rupture of Membranes. Journal of Kunming Medical University,
[18]	Cai Yan . . Journal of Kunming Medical University,
[19]	Pan Guo Yan . . Journal of Kunming Medical University,
[20]	Wang Feng . . Journal of Kunming Medical University,