The Relationship between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis in Kunming Area

Bo-yi LI; Ling NIU; Rong MA; Xian ZHANG; Fang LIU; Yan TANG; Cui-juan MIAO; Zhu-jun HAN; Cheng ZHANG

doi:10.12259/j.issn.2095-610X.S20211112

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Bo-yi LI, Ling NIU, Rong MA, Xian ZHANG, Fang LIU, Yan TANG, Cui-juan MIAO, Zhu-jun HAN, Cheng ZHANG. The Relationship between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis in Kunming Area[J]. Journal of Kunming Medical University. doi: 10.12259/j.issn.2095-610X.S20211112

Citation:

Bo-yi LI, Ling NIU, Rong MA, Xian ZHANG, Fang LIU, Yan TANG, Cui-juan MIAO, Zhu-jun HAN, Cheng ZHANG. The Relationship between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis in Kunming Area[J]. Journal of Kunming Medical University. doi: 10.12259/j.issn.2095-610X.S20211112

Citation:

Bo-yi LI, Ling NIU, Rong MA, Xian ZHANG, Fang LIU, Yan TANG, Cui-juan MIAO, Zhu-jun HAN, Cheng ZHANG. The Relationship between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis in Kunming Area[J]. Journal of Kunming Medical University. doi: 10.12259/j.issn.2095-610X.S20211112

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The Relationship between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis in Kunming Area

doi: 10.12259/j.issn.2095-610X.S20211112

Department of Endocrinology，First People’s Hospital of Kunming，Yunnan Kunming 650011，China

Received Date: 2021-10-23

Abstract

Abstract

Objective To study the relationship between the T950C polymorphism in the promoter region of the osteoprotegerin （OPG） gene and type 2 diabetes mellitus （T2DM） with osteoporosis in Kunming, and to explore the genetic susceptibility factors of T2DM with osteoporosis. Methods （1） The polymerase chain reaction （PCR-RFLP） technique was used to detect the OPG gene T950C polymorphism about 61 cases in the T2DM without osteoporosis group （group A）, 111 cases in the T2DM combined with osteopenia group （group B）, and T2DM with osteoporosis group （ Group C） 65 cases. The OPG gene T950C polymorphism was analyzed in the 3 groups of people. （2） We compare the 3 groups of patients' clinical indicators including gender, age, diabetes course, blood pressure, height, weight, body mass index （ BMI）, fasting blood glucose （FPG）, glycosylated hemoglobin （HbA1c）, blood calcium, blood lipids, blood uric acid （UA）, vitamin D concentration, estrogen, testosterone levels, high-sensitivity C-reactive protein （Hs-CRP）, fibrin Original （FIB）, oral glucose tolerance （OGTT） -0h, 2h blood glucose, 0h, 2h insulin （INS） levels and insulin resistance index （HOMA-IR）. （3） We compared the bone mineral density among different genotypes of OPG gene T950C. （4） Multivariate logistic regression analysis was performed on the genotype of OPG gene T950C and the indicators with statistical differences between groups. Results (1） In patients with T2DM and T2DM with osteoporosis in Kunming, the main genotype of OPG gene T950C was TC. （2） There was no statistical difference in the genotype frequency and allele frequency of the three groups of OPG gene T950C （P > 0.05）. （3） The comparison of age, height, weight, and BMI of the three groups of patients, the difference was statistically significant （P < 0.05）, and the differences were statistically significant in each other （P < 0.05）. （4） Comparing the three genotypes of OPG gene T950C, there was no statistically significant difference in bone mineral density （P > 0.05）. （5） Multivariate logistic regression analysis showed that age and somking can enter the regression equation, while OPG gene T950C genotype did not enter the regression equation. Conclusions (1） In patients with T2DM and T2DM with osteoporosis in Kunming, the genotype of OPG gene T950C is mainly TC type. （2） There is no statistical difference in the genotype frequency and allele frequency of the three groups of OPG gene T950C. Comparing the three genotypes of OPG gene T950C, there is no statistically significant difference in bone mineral density. The genotype of OPG gene T950C may have nothing to do with the genetic susceptibility of T2DM and T2DM with osteoporosis in Kunming （3） Age and smoking are independent risk factor for patients with T2DM and osteoporosis in Kunming.
- Osteoprotegerin （OPG）,
- Gene promoter region T950C Polymorphism,
- Type 2 diabetic,
- Osteoporosis

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References(19)

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