Expression of ICAM-1, MMP-9 and NF-κB in Cholangiocarcinoma and Their Relationship with Immunosuppressive Cells in Peripheral Blood

Zhi-qiang LI; Hong-qiang GAO; Jing-feng LI; Jie-qi YU; Dong-dong WANG; Gang CHEN; Guang CHU

doi:10.12259/j.issn.2095-610X.S20211117

Volume 42 Issue 11

Nov. 2021

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Article Navigation > Journal of Kunming Medical University > 2021 > 42(11): 93-98

Zhi-qiang LI, Hong-qiang GAO, Jing-feng LI, Jie-qi YU, Dong-dong WANG, Gang CHEN, Guang CHU. Expression of ICAM-1, MMP-9 and NF-κB in Cholangiocarcinoma and Their Relationship with Immunosuppressive Cells in Peripheral Blood[J]. Journal of Kunming Medical University, 2021, 42(11): 93-98. doi: 10.12259/j.issn.2095-610X.S20211117

Citation:

Zhi-qiang LI, Hong-qiang GAO, Jing-feng LI, Jie-qi YU, Dong-dong WANG, Gang CHEN, Guang CHU. Expression of ICAM-1, MMP-9 and NF-κB in Cholangiocarcinoma and Their Relationship with Immunosuppressive Cells in Peripheral Blood[J]. Journal of Kunming Medical University, 2021, 42(11): 93-98. doi: 10.12259/j.issn.2095-610X.S20211117

Citation:

Zhi-qiang LI, Hong-qiang GAO, Jing-feng LI, Jie-qi YU, Dong-dong WANG, Gang CHEN, Guang CHU. Expression of ICAM-1, MMP-9 and NF-κB in Cholangiocarcinoma and Their Relationship with Immunosuppressive Cells in Peripheral Blood[J]. Journal of Kunming Medical University, 2021, 42(11): 93-98. doi: 10.12259/j.issn.2095-610X.S20211117

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Expression of ICAM-1, MMP-9 and NF-κB in Cholangiocarcinoma and Their Relationship with Immunosuppressive Cells in Peripheral Blood

doi: 10.12259/j.issn.2095-610X.S20211117

Dept. of Hepatobiliary and Pancreatic Surgery，The Calmette Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650224，China

Received Date: 2021-09-11
Publish Date: 2021-11-25

Abstract

Abstract

Objective To investigate the expression of intercellular adhesion molecule-1 （ICAM-1）, matrix metalloproteinase-9(MMP-9） and NF-κB in cholangiocarcinoma and the relationship between CD14⁺CD11b⁺HAL-DR^-MDSC in peripheral blood. Methods From December 2018 to December 2019, 60 patients with cholangiocarcinoma were selected. Immunohistochemistry was used to detect ICAM-1 protein, MMP-9 protein and NF-κB protein in cholangiocarcinoma and adjacent normal tissues, and flow cytometry was used to detect the ratio of CD14⁺CD11b⁺HAL-DR^-MDSC in patients’peripheral blood. The expression of ICAM-1 protein, MMP-9 protein and NF-κB protein in cholangiocarcinoma and adjacent tissues were compared; the expression of ICAM-1 protein, MMP-9 protein and NF-κB protein in different pathological features and the ratio of CD14⁺CD11b⁺HAL-DR^-MDSC in peripheral blood were compared; the expression of ICAM-1 protein, MMP-9 protein and NF-κB protein was correlated with the ratio of CD14⁺CD11b⁺HAL-DR^-MDSC in peripheral blood. Results The positive rates of ICAM-1 protein, MMP-9 protein and NF-κB protein in cholangiocarcinoma were higher than those in normal group （P < 0.05）. There was no significant difference in the expression of ICAM-1 protein in different gender, age, pathological grade and lymph node metastasis （ P > 0.05）; the positive rate of ICAM-1 protein expression in stage III-IV was higher than that in stage I-II （ P < 0.05）. There was no significant difference in the expression of MMP-9 between different genders, ages, pathological grades and clinical stages （ P > 0.05）; the positive rate of MMP-9 in lymph node metastasis was higher than that in non lymph node metastasis （ P < 0.05）. There was no significant difference in the expression of NF-κB protein between different genders, ages, pathological grades and lymph node metastasis （ P > 0.05）; the positive rate of NF-κB protein expression in stage III-IV was higher than that in stage I-II （ P < 0.05）. There was no significant difference in the proportion of CD14 ⁺CD11b⁺HAL-DR^-MDSC in different sex, age, pathological grade and lymph node metastasis （P > 0.05）; the proportion of CD14 ⁺CD11b⁺HAL-DR^-MDSC in stage III-IV was higher than that in stage I-II （P < 0.05）. The expression of ICAM-1, MMP-9 and NF-κB was positively correlated with the ratio of CD14 ⁺CD11b⁺HAL-DR^-MDSC. Conclusion ICAM-1 protein, MMP-9 protein and NF-κB protein are highly expressed in cholangiocarcinoma, the ratio of CD14⁺CD11b⁺HAL-DR^-MDSC in peripheral blood is increased, and the expression of ICAM-1 protein, MMP-9 protein and NF-κB protein is positively correlated with the ratio of CD14⁺CD11b⁺HAL-DR^-MDSC.
- Intercellular adhesion molecule-1,
- Matrix metalloproteinase-9,
- NF - κ B,
- Cholangiocarcinoma,
- CD14⁺CD11b⁺HAL-DR⁻ myelogenous suppressor cell

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