Iodine-125 Seed Inhibits the Proliferation and Invasion of Gastric Cancer Cells by Regulating MicroRNA-193b-5p Expression

Zhenhuan MA; Zhen LI; Xianglin ZHOU; Guojian LI; Guokai YANG; Jia WAN; Lingjuan DU; Yong YANG

doi:10.12259/j.issn.2095-610X.S20220120

Volume 43 Issue 1

Jan. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(1): 8-13

Zhenhuan MA, Zhen LI, Xianglin ZHOU, Guojian LI, Guokai YANG, Jia WAN, Lingjuan DU, Yong YANG. Iodine-125 Seed Inhibits the Proliferation and Invasion of Gastric Cancer Cells by Regulating MicroRNA-193b-5p Expression[J]. Journal of Kunming Medical University, 2022, 43(1): 8-13. doi: 10.12259/j.issn.2095-610X.S20220120

Citation:

Zhenhuan MA, Zhen LI, Xianglin ZHOU, Guojian LI, Guokai YANG, Jia WAN, Lingjuan DU, Yong YANG. Iodine-125 Seed Inhibits the Proliferation and Invasion of Gastric Cancer Cells by Regulating MicroRNA-193b-5p Expression[J]. Journal of Kunming Medical University, 2022, 43(1): 8-13. doi: 10.12259/j.issn.2095-610X.S20220120

Citation:

Zhenhuan MA, Zhen LI, Xianglin ZHOU, Guojian LI, Guokai YANG, Jia WAN, Lingjuan DU, Yong YANG. Iodine-125 Seed Inhibits the Proliferation and Invasion of Gastric Cancer Cells by Regulating MicroRNA-193b-5p Expression[J]. Journal of Kunming Medical University, 2022, 43(1): 8-13. doi: 10.12259/j.issn.2095-610X.S20220120

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Iodine-125 Seed Inhibits the Proliferation and Invasion of Gastric Cancer Cells by Regulating MicroRNA-193b-5p Expression

doi: 10.12259/j.issn.2095-610X.S20220120

Zhenhuan MA^{1, 2, 3},
Zhen LI^{1, 2},
Xianglin ZHOU³,
Guojian LI^{1, 2},
Guokai YANG^{1, 2},
Jia WAN^{1, 2},
Lingjuan DU^{1, 2},
Yong YANG^{1, 2
,
,}

1.
The 3rd Department. of General Surgery，The Affiliated Hospital of Yunnan University，Kunming Yunnan 650021
2.
The 3rd Department of General Surgery，The 4th Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650021
3.
Clinical Medical College，Dali University，Dali Yunnan 671003，China

Received Date: 2021-12-27
Available Online: 2021-12-20
Publish Date: 2022-01-15

Abstract

Abstract

Objective To investigate the role of microRNA （miRNA, miR）-193b-5p in the process of iodine-125 particle radiotherapy for gastric cancer. Methods Gastric cancer cell line BGC-823 was used as the research object and iodine-125 seed in vitro irradiation model was established, and the expression profile of miRNA was detected by small molecule RNA sequencing. The expression of miR-193b-5p was analyzed by quantitative real time polymerase chain reaction （RT-qPCR）. MiR-193b-5p knockdown cell and control cell lines （knockdown group and control group） were established in gastric cancer cell line BGC-823 by hsa-miR-193b-5p inhibitor and NC inhibitor. Cell proliferation was analyzed by methyl thiazolyl tetrazolium （MTT） assay in the two groups. Cell invasion was analyzed by Transwell. Result Compared with the expression level of miR-193b-5p in the non-irradiated group （0.173±0.045）, the expression of miR-193b-5p in the iodine-125 irradiated group （0.853±0.180） was significantly increased （t = 6.371, P < 0.05）. Compared with the expression of miR-193b-5p in the control group （12.219±2.464）, the expression of miR-193b-5p in the knockdown group （1.264±0.311） was significantly decreased （t = -7.640, P < 0.05）. Compared with the control group （0.424±0.246）, the proliferation of knockdown group （0.574±0.382）was significantly increased （t = 3.927, P < 0.05）. Compared with the control group （40±7）, the number of cell invasion in the knockdown group （222+23） was significantly increased （t = -13.293, P < 0.05）. Conclusion Iodine-125 seed suppresses the proliferation and invasion of gastric cancer cells by regulating the expression of miR-193b-5p in the process of radiotherapy for gastric cancer.
- Iodine-125 seed,
- Gastric cancer,
- microRNA-193b-5p,
- Cell proliferation,
- Cell invasion

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