Methamphetamine Alters Methylation Modifications of Synaptic Plasticity Genes in Addicted Mice

Junhong MAO; Danfeng LU; Yu XU; Yicong DAI; Zherui ZHANG; Yue LI; Kunhua WANG

doi:10.12259/j.issn.2095-610X.S20220121

Volume 43 Issue 1

Jan. 2022

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Junhong MAO, Danfeng LU, Yu XU, Yicong DAI, Zherui ZHANG, Yue LI, Kunhua WANG. Methamphetamine Alters Methylation Modifications of Synaptic Plasticity Genes in Addicted Mice[J]. Journal of Kunming Medical University, 2022, 43(1): 1-7. doi: 10.12259/j.issn.2095-610X.S20220121

Citation:

Junhong MAO, Danfeng LU, Yu XU, Yicong DAI, Zherui ZHANG, Yue LI, Kunhua WANG. Methamphetamine Alters Methylation Modifications of Synaptic Plasticity Genes in Addicted Mice[J]. Journal of Kunming Medical University, 2022, 43(1): 1-7. doi: 10.12259/j.issn.2095-610X.S20220121

Citation:

Junhong MAO, Danfeng LU, Yu XU, Yicong DAI, Zherui ZHANG, Yue LI, Kunhua WANG. Methamphetamine Alters Methylation Modifications of Synaptic Plasticity Genes in Addicted Mice[J]. Journal of Kunming Medical University, 2022, 43(1): 1-7. doi: 10.12259/j.issn.2095-610X.S20220121

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Methamphetamine Alters Methylation Modifications of Synaptic Plasticity Genes in Addicted Mice

doi: 10.12259/j.issn.2095-610X.S20220121

Junhong MAO^{1, 2},
Danfeng LU¹,
Yu XU^{1, 2},
Yicong DAI^{1, 2},
Zherui ZHANG^{1, 2},
Yue LI^{1, 2},
Kunhua WANG^{1, 3
,
,}

1.
NHC Key Laboratory of Drug Addiction Medicine，Kunming Medical University，Kunming Yunnan 650500
2.
Dept. of Gastrointestinal and Hernia Surgery，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
3.
Party and Administration Office of Yunnan University，Kunming Yunnan 650032，China

Received Date: 2021-12-10
Available Online: 2021-12-20
Publish Date: 2022-01-15

Abstract

Abstract

Objective To investigate the expression changes of synaptic plasticity genes in chronic methamphetamine （MA） addicted mice. Methods C57BL/6J mice were selected to simulate the human drug addiction model, and the drugs were injected intraperitoneally with 5 mg/kg, 10 mg/kg or normal saline. The cerebral cortex and hippocampus of mice were selected, and the gene expression level of synaptic plasticity was detected by bisulfite treated genomic DNA and methylation specific PCR （MethylmionSpecificPCR, MSP）. The sequencing results were compared and DNA methylation was analyzed by BiQ-Analyzer software. Results Compared to mice in the saline-treated group, MA addiction group mice showed increased methylation modification of the Egr2 （P = 0.064） gene promoter CpG site and decreased methylation modification of the Eln （P = 0.083） gene promoter in the cerebral cortex; in hippocampal tissue, methylation modification of the Arc （P = 0.025） and Egr2 （P = 0.034） genes was increased, while Eln （P = 0.063） gene methylation modifications were decreased. Conclusion Methylation of synaptic plasticity genes may be involved in the formation of MA addiction mechanism.
- Methamphetamine,
- Synaptic plasticity,
- Immediate early gene,
- DNA Methylation.

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References(25)

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