Platelet-rich Plasma Promotes the Proliferation of Human Endometrial Mesenchymal Stem Cells (EnMSCs) through the PI3K/AKT/mTOR Signaling Pathway

Xiaohan WANG; Shanmao MOU; Cuifang HAO; Linlin REN; Min WANG; Tong ZHAO

doi:10.12259/j.issn.2095-610X.S20220126

Volume 43 Issue 1

Jan. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(1): 26-32

Xiaohan WANG, Shanmao MOU, Cuifang HAO, Linlin REN, Min WANG, Tong ZHAO. Platelet-rich Plasma Promotes the Proliferation of Human Endometrial Mesenchymal Stem Cells (EnMSCs) through the PI3K/AKT/mTOR Signaling Pathway[J]. Journal of Kunming Medical University, 2022, 43(1): 26-32. doi: 10.12259/j.issn.2095-610X.S20220126

Citation:

Xiaohan WANG, Shanmao MOU, Cuifang HAO, Linlin REN, Min WANG, Tong ZHAO. Platelet-rich Plasma Promotes the Proliferation of Human Endometrial Mesenchymal Stem Cells (EnMSCs) through the PI3K/AKT/mTOR Signaling Pathway[J]. Journal of Kunming Medical University, 2022, 43(1): 26-32. doi: 10.12259/j.issn.2095-610X.S20220126

Citation:

Xiaohan WANG, Shanmao MOU, Cuifang HAO, Linlin REN, Min WANG, Tong ZHAO. Platelet-rich Plasma Promotes the Proliferation of Human Endometrial Mesenchymal Stem Cells (EnMSCs) through the PI3K/AKT/mTOR Signaling Pathway[J]. Journal of Kunming Medical University, 2022, 43(1): 26-32. doi: 10.12259/j.issn.2095-610X.S20220126

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Platelet-rich Plasma Promotes the Proliferation of Human Endometrial Mesenchymal Stem Cells (EnMSCs) through the PI3K/AKT/mTOR Signaling Pathway

doi: 10.12259/j.issn.2095-610X.S20220126

1.
Dept. of Obstetrics
2.
Dept. of Encephalopathy，Rizhao Hospital of Traditional Chinese Medicine，Rizhao Shandong 276800
3.
Center for Reproductive Medicine，Qingdao Women and Children’s Hospital，Qingdao University，Qingdao Shandong 266011
4.
Center for Reproductive Medicine，Yantai Yuhuangding Hospital Affiliated to Qingdao University， Yantai Shandong 264000，China

Received Date: 2021-12-18
Publish Date: 2022-01-25

Abstract

Abstract

Objective To explore the mechanism of PRP promoting EnMSCs proliferation, and to provide a theoretical basis for the expansion and clinical application of EnMSCs. Methods EnMSCs were randomly divided into the control group, 2%PRP group and 2%PRP + LY294002 group. Cell proliferation was detected by CCK-8 method. Cell cycle was monitored by flow cytometry and protein expressions of p-PI3K, AKT, p-AKT, mTOR and p-mTOR were detected by Western Blot. Results （1） CCK-8 results: Compared with the control group, the proliferation level of EnMSCs in 2%PRP group was significantly higher （P < 0.05）. Compared with the 2%PRP group, the proliferation level of EnMSCs in the 2%PRP+LY294002 group was significantly lower （ P < 0.05）. （2） The results of cell cycle analysis by flow cytometry showed that the proportion of cells in G2/M phase in the 2% PRP group was significantly higher than that in the control group and 2% PRP+LY294002 group, and the proportion of cells in G0/G1 phase was significantly lower than that in the control group and 2% PRP+ LY294002 group. All of these had statistically significant differences （ P < 0.05）. （3） The protein expressions of p-PI3K, AKT, p-AKT, mTOR and p-mTOR in EnMSCs in the 2%PRP group were significantly higher than those in the control group and the 2%PRP+LY294002 group, with statistical significance （ P < 0.05）. Conclusion The proliferation of EnMSCs is regulated by the PI3K/Akt/mTOR signaling pathway. PRP activates this pathway by promoting the protein expression and phosphorylation of AKT and mTOR, thereby promoting the proliferation of EnMSCs.
- Platelet-rich plasma,
- Endometrial mesenchymal stem cells,
- PI3K/AKT/mTOR,
- Cell proliferation,
- Cell cycle.

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References(22)

References

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