Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women

Xiaohong LI; Lin ZHAO; Xinwen ZHANG; Zhuo CHEN; Runmei MA

doi:10.12259/j.issn.2095-610X.S20220140

Volume 43 Issue 1

Jan. 2022

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Xiaohong LI, Lin ZHAO, Xinwen ZHANG, Zhuo CHEN, Runmei MA. Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women[J]. Journal of Kunming Medical University, 2022, 43(1): 48-52. doi: 10.12259/j.issn.2095-610X.S20220140

Citation:

Xiaohong LI, Lin ZHAO, Xinwen ZHANG, Zhuo CHEN, Runmei MA. Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women[J]. Journal of Kunming Medical University, 2022, 43(1): 48-52. doi: 10.12259/j.issn.2095-610X.S20220140

Citation:

Xiaohong LI, Lin ZHAO, Xinwen ZHANG, Zhuo CHEN, Runmei MA. Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women[J]. Journal of Kunming Medical University, 2022, 43(1): 48-52. doi: 10.12259/j.issn.2095-610X.S20220140

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Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women

doi: 10.12259/j.issn.2095-610X.S20220140

Dept. of Obstetrics and Gynecology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2021-12-06
Available Online: 2022-01-15
Publish Date: 2022-01-15

Abstract

Abstract

Objective To investigate the relationship between chemerin and insulin resistance in GDM by detecting the expression of insulin receptor substrate-1 and tyrosine phosphorylation in omental adipose tissue. Methods Subjects were divided into the GDM group 24 and the normal control group with 22 NGT. Two grams of omental adipocytes were collected during the cesarean section. The expression of chemerin and IRS-1 in placental tissues was detected with Western blot and qPCR, and the tyrosine phosphorylation of IRS-1 was detected with the immunoprecipitation. Results The expression of chemerin protein in retinal adipocytes in GDM group was significantly increased （2.99 （0.04, 7.90） vs 2.21 （0.02, 13.30）, P = 0.010）. The protein expression of IRS-1 （3.52（2.51, 4.49） vs 5.47 （4.04, 8.98）, P = 0.024） was significantly decreased in GDM group. The phosphorylation of IRS-1 tyrosine was significantly decreased in GDM group （0.60（0.01, 1.74） vs 4.06 （0.06, 54.41）, P = 0.012）. Correlation analysis showed that chemerin protein expression was closely related to OGTT2h glucose. Chemerin mRNA was closely correlated with prenatal BMI. Chemerin protein expression was positively correlated with IRS-1 protein. IRS-1 tyrosine phosphorylation was negatively correlated with OGTT 0 h PG and OGTT 2 h PG. Conclusion The expression of chemerin in omental adipose tissue is closely related to IRS-1 signaling, which is one of the factors causing insulin resistance in GDM.
- GDM,
- Chemerin,
- IRS-1,
- Tyrosine phosphorylation,
- Insulin resistance

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References(17)

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