Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women

Xiaohong LI; Lin ZHAO; Xinwen ZHANG; Zhuo CHEN; Runmei MA

doi:10.12259/j.issn.2095-610X.S20220140

Volume 43 Issue 1

Jan. 2022

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Xiaohong LI, Lin ZHAO, Xinwen ZHANG, Zhuo CHEN, Runmei MA. Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women[J]. Journal of Kunming Medical University, 2022, 43(1): 48-52. doi: 10.12259/j.issn.2095-610X.S20220140

Citation:

Xiaohong LI, Lin ZHAO, Xinwen ZHANG, Zhuo CHEN, Runmei MA. Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women[J]. Journal of Kunming Medical University, 2022, 43(1): 48-52. doi: 10.12259/j.issn.2095-610X.S20220140

Citation:

Xiaohong LI, Lin ZHAO, Xinwen ZHANG, Zhuo CHEN, Runmei MA. Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women[J]. Journal of Kunming Medical University, 2022, 43(1): 48-52. doi: 10.12259/j.issn.2095-610X.S20220140

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Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women

doi: 10.12259/j.issn.2095-610X.S20220140

Dept. of Obstetrics and Gynecology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2021-12-06
Available Online: 2022-01-15
Publish Date: 2022-01-15

Abstract

Abstract

Objective To investigate the relationship between chemerin and insulin resistance in GDM by detecting the expression of insulin receptor substrate-1 and tyrosine phosphorylation in omental adipose tissue. Methods Subjects were divided into the GDM group 24 and the normal control group with 22 NGT. Two grams of omental adipocytes were collected during the cesarean section. The expression of chemerin and IRS-1 in placental tissues was detected with Western blot and qPCR, and the tyrosine phosphorylation of IRS-1 was detected with the immunoprecipitation. Results The expression of chemerin protein in retinal adipocytes in GDM group was significantly increased （2.99 （0.04, 7.90） vs 2.21 （0.02, 13.30）, P = 0.010）. The protein expression of IRS-1 （3.52（2.51, 4.49） vs 5.47 （4.04, 8.98）, P = 0.024） was significantly decreased in GDM group. The phosphorylation of IRS-1 tyrosine was significantly decreased in GDM group （0.60（0.01, 1.74） vs 4.06 （0.06, 54.41）, P = 0.012）. Correlation analysis showed that chemerin protein expression was closely related to OGTT2h glucose. Chemerin mRNA was closely correlated with prenatal BMI. Chemerin protein expression was positively correlated with IRS-1 protein. IRS-1 tyrosine phosphorylation was negatively correlated with OGTT 0 h PG and OGTT 2 h PG. Conclusion The expression of chemerin in omental adipose tissue is closely related to IRS-1 signaling, which is one of the factors causing insulin resistance in GDM.
- GDM,
- Chemerin,
- IRS-1,
- Tyrosine phosphorylation,
- Insulin resistance

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References(17)

References

[1]	程飞,郭晓蕙,杨慧霞,等. 妊娠糖尿病患者血清脂联素水平及其临床意义[J]. 北京医学,2009,6(31):337-339.
[2]	李晓红,马润玫,陈卓. 肥胖、胰岛素抵抗对妊娠期糖尿病患者血清网膜素-1水平的影响[J]. 昆明医科大学学报,2017,38(5):64-67. doi: 10.3969/j.issn.1003-4706.2017.05.014
[3]	A A Roman,S D Parlee,C J Sinal,et al. Chemerin:A potential endocrine link between obesity and type 2 diabetes[J]. Endocrine,2012,2(42):243-251.
[4]	S S Fatima,R Rehman,M Baig,et al. New roles of the multidimensional adipokine:Chemerin[J]. Peptides,2014,12(62):15-20.
[5]	Ferland D J,Mullick A E,Watts S W. Chemerin as a driver of hypertension:A consideration[J]. American Journal of Hypertension,2020,33(11):975-986. doi: 10.1093/ajh/hpaa084
[6]	Wang D,Wang H,Li M,et al. ,Chemerin levels and its genetic variants are associated with gestational diabetes mellitus:A hospital-based study in a Chinese cohort[J]. Gene,2021,10(8):145888E.
[7]	李晓红,陈卓,马润玫. 血清趋化素与妊娠期肥胖及胰岛素抵抗的关系[J]. 妇女保健,2013,28(36):5941-5944.
[8]	Michiko Takahashi,Yutaka Takahashi,Kenichi Takahashi,et al. Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes[J]. FEBS Letters,2008,582(5):573-578. doi: 10.1016/j.febslet.2008.01.023
[9]	Yashdeep G,Bharti K,Manash PB,et al. Updated guidelines on screening for gestational diabetes[J]. International Journal of Women’s Health,2015,19(7):539-550.
[10]	Jordan S D,Krüger M,Willmes D M,et al. Obesity-induced over-expression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism[J]. Nat Cell Biol,2011,13(4):434-446. doi: 10.1038/ncb2211
[11]	Hyun-Young Shin,Duk C Lee,Sang H Chu,et al. Chemerin levels are positively correlated with abdominal visceral fat accumulation[J]. Clinical Endocrinology,2012,77(1):47-50. doi: 10.1111/j.1365-2265.2011.04217.x
[12]	Anna Szpakowicz,Malgorzata Szpakowicz,Magda Lapinska,et al. Serum chemerin concentration is associated with proinflammatory status in chronic coronary syndrome[J]. Biomolecules,2021,11(8):1149. doi: 10.3390/biom11081149
[13]	Sabrina Bauer,Margarita Bala,Andrea Kopp,et al. Adipocyte chemerin release is induced by insulin without being translated to higher levels in vivo[J]. Eur J Clin Invest,2012,42(11):1213-1220. doi: 10.1111/j.1365-2362.2012.02713.x
[14]	Sabrina Bauer,Josef Wanninger,Sandra Schmidhofer,et al. Sterol regulatory element-binding protein 2 (SREBP2) activation after excess triglyceride storage induces chemerin in hypertrophic adipocytes[J]. Endocrinology,2011,152(1):26-35. doi: 10.1210/en.2010-1157
[15]	Kennedy A J,Davenport A P. Chemerin receptors CMKLR1 (Chemerin1) and GPR1 (Chemerin2) nomenclature,pharmacology,function[J]. Pharmacol,2018,70(1):174-196.
[16]	Koelman L,Reichmann R,Börnhorst C,et al. Determinants of elevated chemerin as a novel biomarker of immunometabolism:Data from a large population-based cohort[J]. Endocrine Connections,2021,20(9):1200-1211.
[17]	Ays, Bulut ,Gülçin Akca ,et al. The significance of blood and salivary oxidative stress markers and chemerin in gestational diabetes mellitus[J]. Taiwan residents Journal of Obstetrics & Gynecology,2021,4(60):695-699.

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