Effects of Micafungin on the Activity of Candida Smooth in Macrophages

Weijia ZHAO; Hongbin LI; Zonghan CHEN

doi:10.12259/j.issn.2095-610X.S20220142

Volume 43 Issue 1

Jan. 2022

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Weijia ZHAO, Hongbin LI, Zonghan CHEN. Effects of Micafungin on the Activity of Candida Smooth in Macrophages[J]. Journal of Kunming Medical University, 2022, 43(1): 14-19. doi: 10.12259/j.issn.2095-610X.S20220142

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Weijia ZHAO, Hongbin LI, Zonghan CHEN. Effects of Micafungin on the Activity of Candida Smooth in Macrophages[J]. Journal of Kunming Medical University, 2022, 43(1): 14-19. doi: 10.12259/j.issn.2095-610X.S20220142

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Effects of Micafungin on the Activity of Candida Smooth in Macrophages

doi: 10.12259/j.issn.2095-610X.S20220142

1.
Dept. of Dermatology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan，650032
2.
Higher Education Research and Evaluation Center，Yunnan University of Chinese Medicine，Kunming Yunnan 650504，China

Received Date: 2021-12-12
Available Online: 2022-01-15
Publish Date: 2022-01-15

Abstract

Abstract

Objective To investigate the effects of micafungin on the activity of Candida glabra in macrophages. Methods Macrophages were selected as the research object, and samples were isolated from the skin, vaginal secretion, pus, blood and body cavity fluid. Candida smooth strain and a standard strain ATCC2001 （purchased from the national strain collection center） were identified by biochemistry and molecular biology. Different concentrations of micafungin were used to treat Candida smooth. They were divided into the control group, model group, low concentration group, medium concentration group and high concentration group respectively. SOD activity, MDA content and no content were measured at the different times, and NF was measured- Κ B protein expression levels of p65, BKCa, NLRP3 and ATG5, IL-6, IL-10 and IL-1 β, TNF- α Level. Results Compared with the control group, the activity of SOD stimulated by macrophages in the modeling group was significantly decreased; Compared with the modeling group, the activity of SOD stimulated by macrophages in the low concentration group, medium concentration group and high concentration group was significantly increased, reaching the maximum value at 5 h, with the low concentration group > medium concentration group > high concentration group, and the difference was statistically significant （P < 0.05）. Compared with the control group, the secretion of MDA and NO by macrophages in the modeling group was significantly increased; Compared with the modeling group, the levels of MDA and NO in the low concentration group, medium concentration group and high concentration group were significantly increased, and the order of secretion content was as follows: medium concentration group > high concentration group > low concentration group, the difference was statistically significant （P < 0.05）. The protein expressions of NF- κ B P65, BKca, NLRP3 and Atg5 were significantly increased in the modeling group compared with the control group, and the protein expressions of NF- κ B P65, BKca, NLRP3 and Atg5 were significantly increased in the low-concentration, medium-concentration and high-concentration groups compared with the modeling group. Low concentration group > medium concentration group > high concentration group, the difference was statistically significant （P < 0.05）.Compared with the control group, the level of serum inflammatory factors in macrophages in the model group was significantly increased; Compared with the modeling group, the levels of IL-6, IL-10, IL-1β and TNF-α in macrophages of low concentration group, medium concentration group and high concentration group were significantly increased, and the secretion of inflammatory factors was in the order of low concentration group > medium concentration group > high concentration group, the difference was statistically significant （P < 0.05）. Conclusion Micafengin can effectively stimulate the stress response of macrophages, regulate the function of immune system, inhibit the activity of Candida glabra, and play a better antibacterial effect.
- Micafenjing,
- Activity of Candida smooth,
- Macrophages,
- Protein expression

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