Potential Value of G6PD Activity Detection in the Peripheral Blood for the Diagnosis and Prognosis of Cervical Cancer Patients with High Risk Human Papillomavirus Infection

Honglan CHEN; Yi WU; Tao HU; Yuming WANG

doi:10.12259/j.issn.2095-610X.S20220203

Volume 43 Issue 2

Mar. 2022

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Honglan CHEN, Yi WU, Tao HU, Yuming WANG. Potential Value of G6PD Activity Detection in the Peripheral Blood for the Diagnosis and Prognosis of Cervical Cancer Patients with High Risk Human Papillomavirus Infection[J]. Journal of Kunming Medical University, 2022, 43(2): 89-95. doi: 10.12259/j.issn.2095-610X.S20220203

Citation:

Honglan CHEN, Yi WU, Tao HU, Yuming WANG. Potential Value of G6PD Activity Detection in the Peripheral Blood for the Diagnosis and Prognosis of Cervical Cancer Patients with High Risk Human Papillomavirus Infection[J]. Journal of Kunming Medical University, 2022, 43(2): 89-95. doi: 10.12259/j.issn.2095-610X.S20220203

Citation:

Honglan CHEN, Yi WU, Tao HU, Yuming WANG. Potential Value of G6PD Activity Detection in the Peripheral Blood for the Diagnosis and Prognosis of Cervical Cancer Patients with High Risk Human Papillomavirus Infection[J]. Journal of Kunming Medical University, 2022, 43(2): 89-95. doi: 10.12259/j.issn.2095-610X.S20220203

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Potential Value of G6PD Activity Detection in the Peripheral Blood for the Diagnosis and Prognosis of Cervical Cancer Patients with High Risk Human Papillomavirus Infection

doi: 10.12259/j.issn.2095-610X.S20220203

Honglan CHEN^{1, 2},
Yi WU³,
Tao HU²,
Yuming WANG^{1
,
,}

1.
Dept. of Laboratory Medicine，Second Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101
2.
Dept. of Laboratory Medicine，The Third People’s Hospital of Yunnan Province，Kunming Yunnan 650200
3.
Science and Technology Achievement Incubation Center，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2021-12-05
Available Online: 2022-02-24
Publish Date: 2022-03-04

Abstract

Abstract

Objective To explore the value of detecting G6PD activity in the peripheral blood for the diagnosis and prognosis of cervical cancer patients with high-risk human papillomavirus （HR-HPV） infection. Methods A total of 53 cervical cancer （CC） patients, 58 cervical intraepithelial neoplasia （CIN） patients and 40 health cohorts （HC）, visiting and attending the clinic of Yunnan Third People’s Hospital during June 2014 to December 2015, were employed in this study. HPV Gene Chip Test Kit was used to detect and screen the genotype HPV infection in each group of population. Pathological evaluation and genotyping were performed on surgical specimens of cervical cancer patients. Serum samples were taken from each group of population, the activity of G6PD was detected by immunofluorescence assay, and the correlation analysis was carried out according to the clinical data and the results of G6PD activity. The results were then analyzed based on evidence. Results The activity of G6PD in peripheral blood was the highest in CC group, and there was significant difference to CIN Group and HC Group （P < 0.01） , and CIN Group to HC Group （P < 0.05）. The change of G6PD activity in peripheral blood of CC patients is closely related to the progression of cervical cancer, and the change of G6PD activity in peripheral blood of CIN patients is closely related to the progression of cervical cancer. In CC group and CIN Group, the activity of G6PD in peripheral blood was consistent with HPV16/18 infection, and the relationship between the OS curve and the activity of G6PD in peripheral blood of CC patients infected with HPV 16/18 was analyzed, the 5-year survival rate decreased significantly in patients with high G6PD activity in peripheral blood （P <0.05） . The validity and reliability of G6PD activity detection and / or HPV16/18 infection test are valuable in evidence-based diagnosis for CIN or cervical cancer. Conclusions The activity of G6PD is related to the progression of cervical cancer. The detection of G6PD activity in peripheral blood can be used as one of the diagnostic and prognostic indicators of CIN and HR-HPV-infected cervical cancer, especially HPV 16/18.
- G6PD,
- High-risk human papillomavirus,
- Cervical cancer,
- Prognosis,
- Evidence-based Diagnosis

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References(18)

References

[1]	Berman T A,Schiller J T. Human papillomavirus in cervical cancer and oropharyngeal cancer:One cause,two diseases[J]. Cancer,2017,123(12):2219-2229. doi: 10.1002/cncr.30588
[2]	Joseph A N,Bhatla N. Population-based human papillomavirus testing:The new paradigm for cervical cancer screening[J]. Natl Med J India,2021,34:36-37. doi: 10.4103/0970-258X.323447
[3]	Mammas I N,Sourvinos G,Spandidos D A. Human papilloma virus (HPV) infection in children and adolescents[J]. European Journal of Pediatrics,2009,168(3):267-273. doi: 10.1007/s00431-008-0882-z
[4]	Margaret,E,Cruickshank. The role of human papillomavirus in risk management[J]. Reviews in Gynaecological Practice,2003,3(4):229-233. doi: 10.1016/S1471-7697(03)00057-1
[5]	Munoz N,Bosch F X,De-Sanjose S,et al. Epidemiologic classification of human papillomavirus types associated with cervical cancer[J]. The New England Journal of Medicine,2003,348(6):518-527. doi: 10.1056/NEJMoa021641
[6]	Hu T , Li Y S , Chen B , et al. Elevated glucose-6-phosphate dehydrogenase expression in the cervical cancer cases is associated with the cancerigenic event of high-risk human papillomaviruses[J]. Experimental Biology & Medicine, 2015, 1287-1297.
[7]	Luo Z,Du D,Liu Y,et al. Discovery and characterization of a novel glucose-6-phosphate dehydrogenase (G6PD) inhibitor via high-throughput screening[J]. Bioorg Med Chem Lett,2021,40:127905. doi: 10.1016/j.bmcl.2021.127905
[8]	Hu,T. ,Zhang,C.,Tang,Q.,et al. Variant G6PD levels promote tumor cell proliferation or apoptosis via the STAT3/5 pathway in the human melanoma xenograft mouse model[J]. Bio Med Central,2013,13(1):251. doi: 10.1186/1471-244X-13-251
[9]	苏琼,黄成双,吴柳松,等. G6PD活性在急性白血病患儿中的变化及其临床意义[J]. 中国实验血液学杂志,2018,26(06):1649-1656.
[10]	樊小群,李欢,朱华雄,等. Mir-335-5p靶向G6PD对结肠癌细胞增殖、凋亡的影响[J]. 中国应用生理学杂,2021,37(04):402-406.
[11]	Polat M F,Taysi S,Gul M,et al. Oxidant/antioxidant status in blood of patients with malignant breast tumour and benign breast disease[J]. Cell Biochem Funct,2002,20(4):327-331. doi: 10.1002/cbf.980
[12]	蒋素贞,方艺川,卢丽娜,等. 6-磷酸葡萄糖脱氢酶在子宫内膜癌中的表达[J]. 广东医学,2016,37(22):3400-3403. doi: 10.3969/j.issn.1001-9448.2016.22.027
[13]	Hu T,Chang Y,Xiao Z,et al. miR-1 inhibits progression of high-risk papillomavirus-associated human cervical cancer by targeting G6PD[J]. Oncotarget,2016,7:86103-86116. doi: 10.18632/oncotarget.13344
[14]	中华医学会病理学分会女性生殖系统疾病学组,中国优生科学协会阴道镜与宫颈病理学会病理学组. 宫颈癌及癌前病变病理诊断规范[J]. 中华病理学杂志,2019,48(4):265-269. doi: 10.3760/cma.j.issn.0529-5807.2019.04.001
[15]	Jiang Z,Albanese J,Kesterson J,et al. Monoclonal Antibodies Against Human Papillomavirus E6 and E7 Oncoproteins Inhibit Tumor Growth in Experimental Cervical Cancer[J]. Translational Oncology,2019,12(10):1289-1295. doi: 10.1016/j.tranon.2019.06.003
[16]	He Z,Xia Y,Liu B,et al. Down-regulation of miR-452 is associated with poor prognosis in the non-small-cell lung cancer[J]. J Thorac Dis,2016,8(5):894-900. doi: 10.21037/jtd.2016.03.51
[17]	Goodman,A. HPV testing as a screen for cervical cancer[J]. Bmj,2015,350:2372. doi: 10.1136/bmj.h2372
[18]	Kontostathi G,Zoidakis J,Anagnou N P,et al. Proteomics approaches in cervical cancer:focus on the discovery of biomarkers for diagnosis and drug treatment monitoring.[J]. Expert Rev Proteomics,2016,13:731-745. doi: 10.1080/14789450.2016.1210514

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