Study on Microbiome of Supragingival Plaque of College Students with Gingivitis

Xinyi LI; Yuexiao LI; Lixian LI; Yongping LI; Ying GAO; Yanhong LI

doi:10.12259/j.issn.2095-610X.S20220207

Volume 43 Issue 2

Mar. 2022

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Xinyi LI, Yuexiao LI, Lixian LI, Yongping LI, Ying GAO, Yanhong LI. Study on Microbiome of Supragingival Plaque of College Students with Gingivitis[J]. Journal of Kunming Medical University, 2022, 43(2): 102-106. doi: 10.12259/j.issn.2095-610X.S20220207

Citation:

Xinyi LI, Yuexiao LI, Lixian LI, Yongping LI, Ying GAO, Yanhong LI. Study on Microbiome of Supragingival Plaque of College Students with Gingivitis[J]. Journal of Kunming Medical University, 2022, 43(2): 102-106. doi: 10.12259/j.issn.2095-610X.S20220207

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Study on Microbiome of Supragingival Plaque of College Students with Gingivitis

doi: 10.12259/j.issn.2095-610X.S20220207

Xinyi LI¹,
Yuexiao LI¹,
Lixian LI²,
Yongping LI¹,
Ying GAO^{2
,
,},
Yanhong LI^{1
,
,}

1.
The Affiliated Stomatology Hospital of Kunming Medical University，Kunming Yunnan 650101
2.
Yunnan Baikal Group Health products Co. Ltd.，Kunming Yunnan 650217，China

Received Date: 2021-12-16
Available Online: 2022-02-24
Publish Date: 2022-03-04

Abstract

Abstract

Objective To compare the microbial differences of supragingival plaque between healthy gingiva group and gingivitis group of college students by high-throughput sequencing technology. Methods According to inclusion and exclusion criteria, 30 college students aged 18-25 with healthy gingiva and 33 with gingivitis were recruited from Kunming Medical University. Sequencing of 16S rDNA gene amplicon was performed using MiSeq system, and the diversity, structure and composition of supragingival plaque were compared between the two groups. Results A total of 87, 595 sequences were detected, including 14 phyla and 216 genera. Compared with the healthy gingiva group, the α diversity index in the gingivitis group was higher, and the difference was statistically significant （P < 0.001）. The result of similarity analysis （Anosim） and principal component analysis （PCA） showed that there were significant differences in the structure and composition of the microflora of supragingival plaque between the healthy gingiva group and the gingivitis group （P < 0.001）. The relative abundance of Prevotella, Porphyromonas and Bacillus in gingivitis group was higher, while the relative abundance of Actinomycetes in healthy gingiva group was higher. Conclusion There are significant differences in microbial community composition and structure of supragingival plaque between the healthy gingiva group and the gingivitis group of college students, and the diversity of the gingivitis group is higher.
- Gingivitis,
- High-throughput sequencing,
- Microbiome,
- College students

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References(22)

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