The Spation-temporal Expression of SOX9 during the Development of Mandibular Condylar Cartilage and Tibial Growth Plate Cartilage in SD Rats

Rui MAO; Ying HE; Zichao DAI; Song LI

doi:10.12259/j.issn.2095-610X.S20220210

Volume 43 Issue 2

Mar. 2022

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Rui MAO, Ying HE, Zichao DAI, Song LI. The Spation-temporal Expression of SOX9 during the Development of Mandibular Condylar Cartilage and Tibial Growth Plate Cartilage in SD Rats[J]. Journal of Kunming Medical University, 2022, 43(2): 1-6. doi: 10.12259/j.issn.2095-610X.S20220210

Citation:

Rui MAO, Ying HE, Zichao DAI, Song LI. The Spation-temporal Expression of SOX9 during the Development of Mandibular Condylar Cartilage and Tibial Growth Plate Cartilage in SD Rats[J]. Journal of Kunming Medical University, 2022, 43(2): 1-6. doi: 10.12259/j.issn.2095-610X.S20220210

Citation:

Rui MAO, Ying HE, Zichao DAI, Song LI. The Spation-temporal Expression of SOX9 during the Development of Mandibular Condylar Cartilage and Tibial Growth Plate Cartilage in SD Rats[J]. Journal of Kunming Medical University, 2022, 43(2): 1-6. doi: 10.12259/j.issn.2095-610X.S20220210

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The Spation-temporal Expression of SOX9 during the Development of Mandibular Condylar Cartilage and Tibial Growth Plate Cartilage in SD Rats

doi: 10.12259/j.issn.2095-610X.S20220210

Rui MAO¹,
Ying HE^{1, 2},
Zichao DAI¹,
Song LI^{1
,
,}

1.
School of Stomatology，Kunming Medical University，Kunming Yunnan 650500
2.
Dept. of Outpatient，Yunnan Province Corps Hospital，Chinese People’s Armed Police Forces， Kunming Yunnan 650100，China

Received Date: 2021-12-18
Available Online: 2022-02-24
Publish Date: 2022-03-04

Abstract

Abstract

Objective To explore the spation-temporal expression changes of SOX9during the development of condylar cartilage and tibial growth plate cartilage in SD rats. Methods Thirty adult SD rats were caged at the female-male ratio of 2∶1. The vaginal plug appeared was designated as gestation day 0 （GD0） and the rats were isolated then and raised in the routine way. The embryos were obtained from day 13 to day 21 respectively and the condylar cartilage and tibial growth plate cartilage were harvested. Serial section was performed to obtain the slices of the two kinds of cartilages at different development stages （E13 d-E21 d）. HE staining and immunohistochemical detection were employed to compare the structure distinguish between two kinds of cartilages and examine the expressional changes of SOX9. The data were analyzed by applying single factor analysis of variance and two independent sample t test in SPSS17.0 software package. Results Some morphological differences, mainly in the forms, stratification and arrangements of cells, were observed between the condylar cartilage and the tibial growth plate cartilage during E14 d-E21 d indicated by HE staining. In tibial growth plate cartilage, the SOX9 positive expression in E15 d, E17 d and E20 d were stronger than those in condylar cartilage （P < 0.05）. The distribution of positive cells in tibial growth plate and cartilage condylar cartilage was basically identical. SOX9 was mainly positive in the proliferation layer of the two kinds of cartilage. Conclusions The main structures of condylar cartilage and tibial growth plate cartilage during the development process are similar. The minority distinguished part is the layered form, which indicates different growth characteristics during embryonic development. During embryonic development, SOX9 is expressed in the two kinds of cartilages above and mainly distributes in the proliferative zone, which shows SOX9 is involved in the cell proliferation and differentiation associated with the differentiation of cartilage cell and development of cartilage.
- Condylar cartilage,
- Tibial growth plate cartilage,
- Embryonic development,
- SOX9

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References(13)

References

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