Establishment of Spinal Cord Ischemia Injury Model in Rats by Thoracic Aorta Occlusion Combined with Aortic Bypass

Ke YANG; Junjie FEI; Yuyin DUAN; Xin LI; Zhongcheng WU; Yingshan HUANG

doi:10.12259/j.issn.2095-610X.S20220217

Volume 43 Issue 2

Mar. 2022

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Ke YANG, Junjie FEI, Yuyin DUAN, Xin LI, Zhongcheng WU, Yingshan HUANG. Establishment of Spinal Cord Ischemia Injury Model in Rats by Thoracic Aorta Occlusion Combined with Aortic Bypass[J]. Journal of Kunming Medical University, 2022, 43(2): 18-22. doi: 10.12259/j.issn.2095-610X.S20220217

Citation:

Ke YANG, Junjie FEI, Yuyin DUAN, Xin LI, Zhongcheng WU, Yingshan HUANG. Establishment of Spinal Cord Ischemia Injury Model in Rats by Thoracic Aorta Occlusion Combined with Aortic Bypass[J]. Journal of Kunming Medical University, 2022, 43(2): 18-22. doi: 10.12259/j.issn.2095-610X.S20220217

Citation:

Ke YANG, Junjie FEI, Yuyin DUAN, Xin LI, Zhongcheng WU, Yingshan HUANG. Establishment of Spinal Cord Ischemia Injury Model in Rats by Thoracic Aorta Occlusion Combined with Aortic Bypass[J]. Journal of Kunming Medical University, 2022, 43(2): 18-22. doi: 10.12259/j.issn.2095-610X.S20220217

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Establishment of Spinal Cord Ischemia Injury Model in Rats by Thoracic Aorta Occlusion Combined with Aortic Bypass

doi: 10.12259/j.issn.2095-610X.S20220217

1.
Dept. of Anesthesiology，Yunnan Fuwai Cardiovascular Hospital，Kunming Yunnan 650032
2.
Dept. of Cardio-Vascular Surgery，The Third People’s Hospital of Chengdu，Chengdu Sichuan 610000
3.
Dept. of Cardiology，Yunnan Fuwai Cardiovascular Hospital， Kunming Yunnan 650032，China

Received Date: 2021-12-07
Available Online: 2022-02-24
Publish Date: 2022-03-04

Abstract

Abstract

Objective To modify the rat model of spinal cord ischemia injury. Methods The rats were divided into three groups. In experimental group, thoracic aorta was cross-clamped between T4 and T11, and aortic bypass circulation was established from proximal thoracic aorta to proximal diaphragmatic aorta. The interval of clamping was 20 minutes. In control group, the thoracic aorta （T4） was cross-clamped for 20 minutes.In the sham operation group, only thoracotomy was performed without thoracic aorta cross-clamping. Thefunction of spinal cord was monitored by transcranial motor evoked potential （TC-MEP）. The pathology of spinal cord were observed 7 days after surgery. The locomotor function of spinal cord was evaluated by BBB score at multiple postoperative time. The survival curves were analyzed 28 days after operation. Results The TC-MEP waveform of the experimental group disappeared after 6 minutes, and that of the control group disappeared after 8 minutes until the end of surgery. The slices of spinal cord showed a large number of degeneration and necrosis of neurons in the experimental group. Multiple neurons were necrotic in the control group. The BBB score of the experimental group decreased more obviously than that of the control group. There was a significant difference in the 28-day survival rate between the experimental group and the control group, and （P < 0.05）. Conclusion Thoracic aortic cross-clamping plus aortic bypass is an effective method, with high long-term survival rate.
- Spinal cord ischemia injury,
- Animal model,
- Aortic bypass,
- Aortic cross-clamping

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References(12)

References

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