Effects of Different Accumulation Culture Methods on the Titer of Phage PEf771

Rongyu YANG; Fei SONG; Yunlin WEI; Xianghong YANG; Kaiwen Duan; Yingying XIANG

doi:10.12259/j.issn.2095-610X.S20220218

Volume 43 Issue 2

Mar. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(2): 7-11

Rongyu YANG, Fei SONG, Yunlin WEI, Xianghong YANG, Kaiwen Duan, Yingying XIANG. Effects of Different Accumulation Culture Methods on the Titer of Phage PEf771[J]. Journal of Kunming Medical University, 2022, 43(2): 7-11. doi: 10.12259/j.issn.2095-610X.S20220218

Citation:

Rongyu YANG, Fei SONG, Yunlin WEI, Xianghong YANG, Kaiwen Duan, Yingying XIANG. Effects of Different Accumulation Culture Methods on the Titer of Phage PEf771[J]. Journal of Kunming Medical University, 2022, 43(2): 7-11. doi: 10.12259/j.issn.2095-610X.S20220218

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Effects of Different Accumulation Culture Methods on the Titer of Phage PEf771

doi: 10.12259/j.issn.2095-610X.S20220218

1.
Dept. of Stomatology，Yan’an Hospital of Kunming City，Kunming Yunnan 650031
2.
Dept. of Minimally Invasive Intervention ，The Third Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650106
3.
Faculty of Life Science and Technology, Kunming University of Science and Technology，Kunming Yunnan 650500，China

Received Date: 2021-12-15
Available Online: 2022-02-24
Publish Date: 2022-03-04

Abstract

Abstract

Objective To compare the effects of three different enriching culture methods on the titer of phage PEf771, and discuss the methods to effectively improve the titer of phage PEf771. Methods Through phage PEf771/E. faecalis YN771 proportional method, spot digging method and mitomycin C induction method, phage PEf771 stock solution was added at E. faecalis YN771 different growth stages for enrichment to obtain a higher titer of phage PEf771. Results The titer of PEf771 obtained by PEf771/E. faecalis YN771 = 1∶3 and spot digging method was higher, there was no significant difference between the two methods after expanded culture （P > 0.05）, and the titer reached 10⁸ pfu/mL in 24 hours. Mitomycin C induction method could not significantly improve the titer of PEf771.PEf771 was added in the early growth stage （OD600 = 0.3） and logarithmic stage （OD600 = 1） of E. faecalis YN771 for enrichment culture. The titer of the former was 10¹⁰ pfu/mL after 6 hours of culture, and remained 10¹⁰ pfu/mL after 24 hours, reaching the ideal titer. Conclusion Adding PEf771 in the early stage of E. faecalis YN771 culture （OD600 = 0.3） and enriching culture with PEf771/E. faecalis YN771 = 1∶3 or spot digging method can effectively improve the titer of PEf771. It provides experimental materials and basis for the study of phage PEf771 of E. faecalis YN771 in the treatment of infection caused by E. faecalis YN771.
- Bacteriophage,
- Enrichment,
- Titer

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References(15)

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