miR-145 Regulates the Expression of OCT4 in Human Endometrial Stromal Cells and Promotes the Development of Endometriosis

Suxian ZHANG; Limei FENG; Lu WU; Shaoyan YANG; Qinghua ZHAO

doi:10.12259/j.issn.2095-610X.S20220220

Volume 43 Issue 2

Mar. 2022

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Suxian ZHANG, Limei FENG, Lu WU, Shaoyan YANG, Qinghua ZHAO. miR-145 Regulates the Expression of OCT4 in Human Endometrial Stromal Cells and Promotes the Development of Endometriosis[J]. Journal of Kunming Medical University, 2022, 43(2): 23-33. doi: 10.12259/j.issn.2095-610X.S20220220

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Suxian ZHANG, Limei FENG, Lu WU, Shaoyan YANG, Qinghua ZHAO. miR-145 Regulates the Expression of OCT4 in Human Endometrial Stromal Cells and Promotes the Development of Endometriosis[J]. Journal of Kunming Medical University, 2022, 43(2): 23-33. doi: 10.12259/j.issn.2095-610X.S20220220

Citation:

Suxian ZHANG, Limei FENG, Lu WU, Shaoyan YANG, Qinghua ZHAO. miR-145 Regulates the Expression of OCT4 in Human Endometrial Stromal Cells and Promotes the Development of Endometriosis[J]. Journal of Kunming Medical University, 2022, 43(2): 23-33. doi: 10.12259/j.issn.2095-610X.S20220220

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miR-145 Regulates the Expression of OCT4 in Human Endometrial Stromal Cells and Promotes the Development of Endometriosis

doi: 10.12259/j.issn.2095-610X.S20220220

Suxian ZHANG¹,
Limei FENG²,
Lu WU¹,
Shaoyan YANG^{1
,
,},
Qinghua ZHAO^{1
,
,}

1.
Dept. of Gynecology
2.
Dept. of Pharmacy，The Second Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

Received Date: 2021-12-07
Available Online: 2022-03-04
Publish Date: 2022-03-04

Abstract

Abstract

Objective To further explore the regulatory mechanism of miR-145 in Endometriosis （EMs） through cell experiments. Methods miR-145 or octamer-binding transcription factor 4 （OCT4）gene and protein expression levels in cells were examined using reverse transcription-quantitative PCR and western blotting, respectively. Correlation analyses and dualluciferase reporter assays were performed to assess the association between miR-145 and OCT4. A Cell Counting Kit-8 assay was performed to test cell viability, while cell apoptosis was measured using ﬂow cytometry. Moreover, the migration of hESCs was measured via Transwell assays. Results In cell assays, overexpression of miR-145 signifcantly promoted proliferation and migration （P < 0.01）, but inhibited the apoptosis of hESCs （P < 0.05）. Furthermore, the transfection of hESCs with miR-145 mimics decreased the protein expression levels of OCT4, Bax and MMP1 （P < 0.01）, as well as increased the protein expression of Bcl-2. However, knockdown of miR-145 reversed these results and significantly inhibited the proliferation of hESCs by targeting OCT4 （P < 0.05）. Conclusion The present results suggested that knockdown of miR-145 signifcantly suppressed the development of EMs by targeting OCT4, which may serve as a potential target for the treatment of EMs.
- Endometriosis,
- miRNA-145,
- OCT4,
- EMT,
- Human endometrial stromal cells

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References

[1]	Zhou C F,Liu M J,Wang W,et al. miR-205-5p inhibits human endometriosis progression by targeting ANGPT2 in endometrial stromal cells[J]. Stem Cell Research & Therapy,2019,10(1):287.
[2]	Cipollini M,Luisi S,Piomboni P,et al. Functional polymorphism within NUP210 encoding for nucleoporin GP210 is associated with the risk of endometriosis[J]. Fertility and Sterility,2019,112(2):343. doi: 10.1016/j.fertnstert.2019.04.011
[3]	Cheng F,Lu L,Wang H,Cheng H,et al. Expression and signifcance of miR-126 and miR-145 in infertility due to endometriosis[J]. J Coll Physicians Surg Pak,2019,29:585-587.
[4]	Sanchez A M,Pagliardini L,Cermisoni g C,et al. Does endometriosis influence the embryo quality and/or development? Insights from a large retrospective matched cohort study[J]. Diagnostics (Basel),2020,10:83. doi: 10.3390/diagnostics10020083
[5]	Wang D,Luo Y,Wang g,et al. Circular RNA expression profles and bioinformatics analysis in ovarian endometriosis[J]. Mol genet genomic Med,2019,7:e00756.
[6]	Zhang Y, Yan J and Pan X: miR-141-3p affects apoptosis and migration of endometrial stromal cells by targeting KLF-12[J]. Pflugers Arch, 2019, 471: 1055-1063,
[7]	Tang W,Chen O,Yao F,et al. miR455 targets FABP4 to protect human endometrial stromal cells from cytotoxicity induced by hydrogen peroxide[J]. Mol Med Rep,2019,20:4781-4790.
[8]	Marí-Alexandre J,Carcelén A P,Agababyan C,et al. Interplay between microRNAs and oxidative stress in ovarian conditions with a focus on ovarian cancer and endometriosis[J]. Int J Mol Sci,2019,20:5322. doi: 10.3390/ijms20215322
[9]	Petracco R,Dias A C O,Taylor H,et al. Evaluation of miR-135a/b expression in endo-metriosis lesions[J]. Biomed Rep,2019,11:181-187.
[10]	Nagai T,Ishida C,Nakamura T,et al. Focal adhesion kinase-mediated sequences,including cell adhesion,inflammatory response,and fbrosis,as a therapeutic target in Endometriosis[J]. Reprod Sci,2020,27:1400-1410. doi: 10.1007/s43032-019-00044-1
[11]	Saare M,Rekker K,Laisk-Podar T,et al. Challenges in endometriosis miRNA studies-from tissue heterogeneity to disease specifc miRNAs[J]. Biochim Biophys Acta Mol Basis Dis,2017,1863:2282-2292. doi: 10.1016/j.bbadis.2017.06.018
[12]	Dai Y,Lin X,Xu W,et al. MiR-210-3p protects endometriotic cells from oxidative stress-induced cell cycle arrest by targeting BARD1[J]. Cell Death Dis,2019,10:144. doi: 10.1038/s41419-019-1395-6
[13]	Moga M A,Bălan A,Dimienescu O G,et al. Circulating miRNAs as biomarkers for endo-metriosis and endometriosis-related ovarian cancer-an overview[J]. J Clin Med,2019,8:735. doi: 10.3390/jcm8050735
[14]	Zhang M,Wang S,Tang L,et al. Downregulated circular RNA hsa_circ_0067301 regulates epithelial-mesenchymal transition in endometriosis via the miR-141/Notch signaling pathway[J]. Biochem Biophys Res Commun,2019,514:71-77. doi: 10.1016/j.bbrc.2019.04.109
[15]	Liu Y,Feng W,Liu W,et al. Circulating lncRNA ABHD11-AS1 serves as a biomarker for early pancreatic cancer diagnosis[J]. J Cancer,2019,10:3746-3756. doi: 10.7150/jca.32052
[16]	Bashti O, Noruzinia M, garshasbi M, et al. miR-31and miR-145 as potential non-invasive regulatory biomarkers in patients with endometriosis[j]. Cell J, 2018, 20: 293.
[17]	Cosar E,Mamillapalli R,Ersoy G S,et al. Serum microRNAs as diagnostic markers of endometriosis:A comprehensive array-based analysis[J]. FertilSteril,2016,106:402-;409.
[18]	Michael A K,grand R S,Isbel L,et al. Mechanisms of OCT4-SOX2 motif readout on nucleosomes[J]. Science,2020,368:1460-1465. doi: 10.1126/science.abb0074
[19]	Harpelunde Poulsen K,Nielsen J E,grønkær Toft B,et al. Inﬂuence of Nodal signalling on pluripotency factor expression,tumour cell proliferation and cisplatin-sensitivity in testicular germ cell tumours[J]. BMC Cancer,2020,20:349. doi: 10.1186/s12885-020-06820-6
[20]	Sadeghi F,Peymaeei F,Falahati M,et al. The effect of Candida cell wall beta-glucan on treatment-resistant LL/2 cancer cell line:In vitro evaluation[J]. Mol Biol Rep,2020,47:3653-3661. doi: 10.1007/s11033-020-05459-7
[21]	Chang Y C,Yang Y F,Chiou J,et al. Nonenzymatic function of Aldolase Adownregulates miR-145 to promote the Oct4/DUSP4/TRAF4 axis and the acquisition of lung cancer stemness[J]. Cell Death Dis,2020,11:195. doi: 10.1038/s41419-020-2387-2
[22]	Cao H,Wei Y X,Zhou Q,et al. Inhibitory effect of curcumin in human endometriosis endometrial cells via downregulation of vascular endothelial growth factor[J]. Mol Med Rep,2017,16:5611-5617. doi: 10.3892/mmr.2017.7250
[23]	Vanhie A,O D,Peterse D,Beckers A,et al. Plasma miRNAs as biomarkers for endometriosis[J]. Hum Reprod,2019,34:1650-1660. doi: 10.1093/humrep/dez116
[24]	Wen J,Wang H,Dong T,et al. STAT3-induced upregulation of lncRNA ABHD11-AS1 promotes tumour progression in papillary thyroid carcinoma by regulating miR-1301-3p/STAT3 axis and PI3K/AKT signalling pathway[J]. Cell Prolif,2019,52:e12569. doi: 10.1111/cpr.12569
[25]	He D,Yue Z,Liu L,et al. Long noncoding RNA ABHD11-AS1 promote cells proliferation and invasion of colorectal cancer via regulating the miR-1254-WNT11 pathway[J]. J Cell Physiol,2019,234:12070-12079. doi: 10.1002/jcp.27877
[26]	Coutinho L M,Ferreira M C,Rocha A L L,et al. New biomarkers in endometriosis[J]. Adv Clin Chem,2019,89:59-77.
[27]	Zhu H,Cao X X,Liu J,et al. MicroRNA-488 inhibits endometrial glandular epithelial cell proliferation,migration,and invasion in endometriosis mice via Wnt by inhibiting FZD7[J]. J Cell Mol Med,2019,23:2419-2430. doi: 10.1111/jcmm.14078
[28]	Bjorkman S and Taylor H S. MicroRNAs in endometriosis:Biological function and emerging biomarker candidatesdagger[J]. Biol Reprod,2019,100:1135-1146.
[29]	Zhang H,Li g,Sheng X,et al. Upregulation of miR33b promotes endometriosis via inhibition of Wnt/β-catenin signaling and ZEB1 expression[J]. Mol Med Rep,2019,19:2144-2152.
[30]	Li D,Jiang W,Jiang Y,et al. Preliminary functional inquiryof lncRNA ENST00000433673 in embryo implantation using bioinformatics analysis[J]. Syst Biol Reprod Med,2019,65:164-173. doi: 10.1080/19396368.2018.1563844
[31]	Hsu C Y,Hsieh T H,Er T K,et al. MiR-381 regulates cell motility,growth and colony formationthrough PIK3CA in endometriosis-associated clear cell and endometrioid ovarian cancer[J]. Oncol Rep,2018,40:3734-3742.
[32]	Mashayekhi P,Noruzinia M,Zeinali S,et al. Endometriotic mesenchymal stem cells epigenetic pathogenesis:Deregulation of miR-200b,miR-145,and let7b in a functional imbalanced epigenetic disease[J]. Cell J,2019,21:179-185.
[33]	GE R and GAO G. Anti-antioxidant impacts of circZNF609 silence in HaCaT cells through regulating miR-145[J]. Artif Cells Nanomed Biotechnol,2020,48:384-392. doi: 10.1080/21691401.2019.1709863
[34]	Adammek M,greve B,Kässens N,Schneider C,et al. MicroRNA miR-145 inhibits proliferation,invasiveness,and stem cell phenotype of an in vitro endometriosis model by targeting multiple cytoskeletal elements and pluripotency factors[J]. Fertil Steril,2013,99:1346-1355.e5. doi: 10.1016/j.fertnstert.2012.11.055
[35]	Shan W,Chen W,Zhao X,et al. Long noncoding RNA TUg1 contributes to cerebral ischaemia/reperfusion injury by sponging mir-145 to up-regulate AQP4 expression[J]. J Cell Mol Med,2020,24:250-259. doi: 10.1111/jcmm.14712
[36]	Zhuang X,Tong H,Ding Y,et al. Long noncoding RNA ABHD11-AS1 functions as a competing endogenous RNA to regulate papillary thyroid cancer progression by miR-199a-5p/SLC1A5 axis[J]. Cell Death Dis,2019,10:620. doi: 10.1038/s41419-019-1850-4
[37]	Liu Y,Zhang Z,Yang F,et al. The role of endometrial stem cells in the pathogenesis of endometriosis and their application to its early diagnosis[J]. Biology of Reproduction,2020,102(6):1153-1159.
[38]	Tiwari D,Ray Das C,Sultana R,et al. Impact of modulation of telomerase and cancer stem-cell marker OCT4 axis incervical cancer pathogenesis with underlying HPV16 infection[J]. J Cell Biochem,2019,121:2782-2791.
[39]	Proestling K,Birner P,Balendran S,et al. Enhanced expression of the stemness-related factors OCT4,SOX15 and TWIST1 in ectopic endometrium of endometriosis patients[J]. Reprod Biol Endocrinol,2016,14:81. doi: 10.1186/s12958-016-0215-4
[40]	Chang J H,Au H K,Lee W C,et al. Expression of the pluripotent transcription factor OCT4 promotes cell migration in endometriosis[J]. Fertil Steril,2013,99:1332-1339.e5. doi: 10.1016/j.fertnstert.2012.11.033
[41]	Au H K,Chang J H,Wu Y C,et al. TgF-βI regulates cell migration through pluripotent transcription factor OCT4 in endometriosis[J]. PLoS One,2015,10:e0145256. doi: 10.1371/journal.pone.0145256
[42]	Kang K,Niu B,Wu C,et al. The construction and application of lentiviral overexpression vector of goat miR-204 in testis[J]. Res Vet Sci,2020,130:52-58. doi: 10.1016/j.rvsc.2020.02.014
[43]	Channakkar A S,Singh T,Pattnaik B,et al. MiRNA-137-mediated modulation of mitochondrial dynamics regulates human neural stem cell fate[J]. Stem Cells,2020,38:683-697. doi: 10.1002/stem.3155
[44]	Shariati F,Favaedi R,Ramazanali F,et al. Increased expression of stemness genes REX-1,OCT-4,NANOG,and SOX-2 in women with ovarian endometriosis versus normal endometrium:A case-control study[J]. International Journal of Reproductive Biomedicine,2018,16(12):783-791.
[45]	Jiang Z,Zhang C,Liu X,et al. Dexamethasone inhibits stemness maintenance and enhances chemosensitivity of hepatocellular carcinoma stem cells by inducing deSUMOylation of HIF-1α and Oct4[J]. Int J Oncol,2020,57(3):780-790. doi: 10.3892/ijo.2020.5097

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