miR-142-5p Regulates the Proliferation and Metastasis of Gallbladder Carcinoma Cells through CCND1

Baoquan WANG; Wei ZHANG; Yuan TIAN; Xifeng LEI; Xu WANG

doi:10.12259/j.issn.2095-610X.S20220223

Volume 43 Issue 2

Mar. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(2): 47-53

Baoquan WANG, Wei ZHANG, Yuan TIAN, Xifeng LEI, Xu WANG. miR-142-5p Regulates the Proliferation and Metastasis of Gallbladder Carcinoma Cells through CCND1[J]. Journal of Kunming Medical University, 2022, 43(2): 47-53. doi: 10.12259/j.issn.2095-610X.S20220223

Citation:

Baoquan WANG, Wei ZHANG, Yuan TIAN, Xifeng LEI, Xu WANG. miR-142-5p Regulates the Proliferation and Metastasis of Gallbladder Carcinoma Cells through CCND1[J]. Journal of Kunming Medical University, 2022, 43(2): 47-53. doi: 10.12259/j.issn.2095-610X.S20220223

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miR-142-5p Regulates the Proliferation and Metastasis of Gallbladder Carcinoma Cells through CCND1

doi: 10.12259/j.issn.2095-610X.S20220223

Dept. of General Surgery，Weinan Central Hospital，Weinan Shanxi 714000，China

Received Date: 2021-12-20
Available Online: 2022-02-24
Publish Date: 2022-03-04

Abstract

Abstract

Objective To explore the effect of miR-142-5p on the proliferation and metastasis of gallbladder cancer through CCND1 and to clarify the special mechanism of action. Methods RT-qPCR was performed to identify the differential expression of miR-142-5p in human gallbladder epithelial cells HGBEC and human gallbladder cell lines. Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-142-5p and CCND1. CCK-8 was used for detecting human gallbladder cell proliferation. Transwell and wound healing assays were carried out to detect gallbladder cell migration. Western blot was performed to detect the marker expression of epithelial mesenchymal transition. Results miR-142-5p was significantly low expressed in gallbladder cancer cell lines, and lowest expressed in GBC-SD cells. Dual luciferase reporter gene assays revealed that miR-142-5p was able to inhibit the luciferase activity of wild-type CCND1. Conclusion Overexpressing of miR-142-5p inhibited the proliferation and metastasis of GBC-SD cells, simultaneous overexpression of miR-142-5p and CCDN1 partially reversed the effect of overexpressing CCDN1 on the proliferation and metastasis of GBC-SD cells. We demonstrate that miR-142-5p can inhibit the proliferation and metastasis of gallbladder cancer cells by suppressing CCND1 expression.
- Gallbladder,
- miR-142-5 p,
- CCND1,
- Proliferation,
- Metastasis

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References(20)

References

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