Impact of Acute Non-normovolemic Hemodilution on Perioperative NK Cell, NKT cell and Th1/Th2 in Elderly Patients Undergoing Rectal Carcinoma Radical Resection

Yiyin QU; Siyuan ZHENG; Chunyu DAI; Yi ZHANG

doi:10.12259/j.issn.2095-610X.S20220225

Volume 43 Issue 2

Mar. 2022

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Zhang Hong Shan . Correlation between Serum Uric Acid Levels and Brachial Ankle Pulse Wave Velocity in Essential Hypertension with Ischemic Stroke Patients[J]. Journal of Kunming Medical University, 2016, 37(03).

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Yiyin QU, Siyuan ZHENG, Chunyu DAI, Yi ZHANG. Impact of Acute Non-normovolemic Hemodilution on Perioperative NK Cell, NKT cell and Th1/Th2 in Elderly Patients Undergoing Rectal Carcinoma Radical Resection[J]. Journal of Kunming Medical University, 2022, 43(2): 128-133. doi: 10.12259/j.issn.2095-610X.S20220225

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Impact of Acute Non-normovolemic Hemodilution on Perioperative NK Cell, NKT cell and Th1/Th2 in Elderly Patients Undergoing Rectal Carcinoma Radical Resection

doi: 10.12259/j.issn.2095-610X.S20220225

1.
Dept. of Anesthesiology，The Third Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650118
2.
Dept. of Anesthesiology，Chengdu Second People’s Hospital，Chengdu Sichuan 610017，China

Received Date: 2021-12-06
Available Online: 2022-02-24
Publish Date: 2022-03-04

Abstract

Abstract

Objective To investigate the impact of acute non-normovolemic hemodilution （ANIH） on the numbers of NK cell, the NK cell cytotoxicity, numbers of NKT cell and the ratio of Th1 /Th2 cytokines in elderly patients undergoing rectal carcinoma radical resection. Methods Forty patients undergoing rectal carcinoma radical resection in the final date analysis were divided into two groups: Conventional Infusion （Group I, n = 20） and ANIH （Group II, n = 20）. The venous blood samples were taken at 1 day before surgery （T1）, at the first day after surgery （T2）, the third day after surgery （T3） and the seventh day after surgery （T4） and measured the numbers of NK cell, NKT cell, Th1 and Th2 cytokines as well as the function of NK cell cytotoxicity by flow cytometry. Then we calculated the rate of Th1/Th2 to observe its variation trend. Results Compared with groups at T1, the NK cell numbers of the two groups at T2 and T3 both increased and the growth of cell numbers from group II had significant statistical deference with that of group I at T3 （P < 0.05）. Oppositely the data of both groups reduced at T4 （P < 0.05） and the reduction from group I was obviously greater than group II （P < 0.05）. Compared with T1, the function of NK cell cytotoxicity of both groups decreased at T2, and the change of group I was more obviously （P < 0.01）. At T3 both groups increased markedly and compared with T1 and T2 it had statistical difference （P < 0.05）. The data from group II rised higher than group I （P < 0.05）. The data from both groups decreased at T4, which almost restore to T1 level. Compared with T2, the NKT cell number of both groups elevated at T3 （P < 0.05）, and the augment of group II was more than group I （P < 0.05）. At T4 the number of both groups went lower than at T3, and there was no significant statistical difference between of two groups. The change of Th1/Th2 made no sense from statistical perspective between two groups at any time point （P > 0.05）. Conclusion Compared with conventional infusion, ANIH can protect and improve the perioperation cellular immunity in elderly patients undergoing rectal carcinoma radical resection as an effective measure of blood protection.
- Hemodilution,
- Rectal neoplasms,
- NK cell,
- NKT cell,
- Th1/Th2

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