Salivary Microbial Profiles of Dental Caries among Lisu Preschool Children in Yunnan Province

Xian BAI; Yao CUI; Qing ZHOU; Xiaoli LI; Yuexiao LI; Juan LIU; Shinan ZHANG

doi:10.12259/j.issn.2095-610X.S20220301

Volume 43 Issue 3

Mar. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(3): 86-93

Xian BAI, Yao CUI, Qing ZHOU, Xiaoli LI, Yuexiao LI, Juan LIU, Shinan ZHANG. Salivary Microbial Profiles of Dental Caries among Lisu Preschool Children in Yunnan Province[J]. Journal of Kunming Medical University, 2022, 43(3): 86-93. doi: 10.12259/j.issn.2095-610X.S20220301

Citation:

Xian BAI, Yao CUI, Qing ZHOU, Xiaoli LI, Yuexiao LI, Juan LIU, Shinan ZHANG. Salivary Microbial Profiles of Dental Caries among Lisu Preschool Children in Yunnan Province[J]. Journal of Kunming Medical University, 2022, 43(3): 86-93. doi: 10.12259/j.issn.2095-610X.S20220301

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Salivary Microbial Profiles of Dental Caries among Lisu Preschool Children in Yunnan Province

doi: 10.12259/j.issn.2095-610X.S20220301

1.
Dept. of Pediatric Dentistry，Affiliated Stomatological Hospital of Kunming Medical University，Kunming Yunnan 650106
2.
Shu Hui Dental Clinic，Kunming Yunnan 650032
3.
The First Clinic of Affiliated Stomatological Hospital of Kunming Medical University
4.
Dept. of Preventive Dentistry，Kunming Yunnan 650106，China

Received Date: 2022-01-15
Publish Date: 2022-03-25

Abstract

Abstract

Objective To study the salivary predominant bacteria in 5-year-old Lisu children with and without dental caries in Yunnan Province and to analyze the differences with local Han children. Methods 20 Lisu children with high caries （dmfs≥6） and 20 children without caries （dmfs = 0）were selected from Nujiang Prefecture of Yunnan Province. Local Han children were also selected with the same inclusion criteria as comparison. Saliva samples were collected. The 16SrRNA V4 hyper variable region was sequenced using illumina Hisequencing platform. The community structure and diversity of microorganisms were analyzed using Mothur software. Results A total of 3965 oral microbial species annotations （Operational taxonomic unit , OTU） were obtained based on 97% similarity clustering in Lisu and Han Chinese children with and without dental caries, belonging to 16 phyla, 23 classes, 57 orders, 102 families and 202 genera. The differences in Alpha diversity and richness indices among the four groups were not statistically significant （P > 0.05）, and the differences in principal co-ordinates analysis （PCoA analysis） among the four groups were not statistically significant （ P > 0.05）. The abundance of Streptococcus in the Lisu caries-active group is higher than that of the caries-free group, while the abundance of Haemophilus, Escherichia-Shigella, Fusobacterium, Capnocytophaga, Megasphaera is lower than that of the caries-free group. For caries-active children, the abundance of Gemella was higher in Lisu than in Han, and the abundance of Escherichia-Shigella was lower in Lisu than in Han. Conclusions The diversity, richness and composition of salivary microbial communities were similar among the Lisu and Han children with and without dental caries in Yunnan. However, the dental caries bio-markers of the two ethnic minority were different. The reasons underline the findings need further invesitigation.
- Lisu ethnic minority,
- Children,
- Dental caries,
- 16SrRNA,
- Salivary microorganism

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