Preparation and Stability of Influenza Vaccine Liposomes Modified by PEG6000

Piao LI; Xiaolin LIU; Ai YANG; Qian ZHANG; Weidong LU

doi:10.12259/j.issn.2095-610X.S20220307

Volume 43 Issue 3

Mar. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(3): 32-36

Piao LI, Xiaolin LIU, Ai YANG, Qian ZHANG, Weidong LU. Preparation and Stability of Influenza Vaccine Liposomes Modified by PEG6000[J]. Journal of Kunming Medical University, 2022, 43(3): 32-36. doi: 10.12259/j.issn.2095-610X.S20220307

Citation:

Piao LI, Xiaolin LIU, Ai YANG, Qian ZHANG, Weidong LU. Preparation and Stability of Influenza Vaccine Liposomes Modified by PEG6000[J]. Journal of Kunming Medical University, 2022, 43(3): 32-36. doi: 10.12259/j.issn.2095-610X.S20220307

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Preparation and Stability of Influenza Vaccine Liposomes Modified by PEG6000

doi: 10.12259/j.issn.2095-610X.S20220307

School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-01-12
Available Online: 2022-02-18
Publish Date: 2022-03-22

Abstract

Abstract

Objective To screen out the best method of PEG6000 modified liposomes for influenza vaccine and evaluate its stability. Methods After 7 d of immunization, the optimal molar percentage of PEG6000 and the optimal preparation method were assumed by MTT method. The PEG-modified influenza vaccine liposomes were prepared by freeze-thaw freeze-drying method. The samples were stored at different temperatures （4 ℃, 25±2 ℃, 37±2 ℃）, and the encapsulation rate and thymus index and antibody titer were measured at a fixed time. So the physical and biological stability of the samples were investigated. Results The MTT experiment showed that PEG6000 was the best dosage when the molar percentage of phospholipid was 4% （P < 0.01）, and the best process was post-modification method.The entrapment rate of PEG6000 modified liposome for influenza vaccine was still higher than 80% when stocked at 37±2 ℃ for 6 months.At 25±2 ℃ for 3 months, the antibody titer was more than 4, indicating that the antibody still had nice immune effection. Conclusions PEG6000 best dosage of phospholipid when the molar percentage of PEG6000 is 4%, and post-modification method is the best preparation process. PEG6000 liposome has nice physical stability and biological stability.
- Liposomes,
- PEG6000,
- Influenza vaccine,
- Stability

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References(26)

References

[1]	Wang N,Chen M,Wang T. Liposomes used as a vaccine adjuvant-delivery system:From basics to clinical immunization[J]. Journal of Controlled Release,2019,303(4):130-150.
[2]	Chen L,Wu Z,Wu X,et al. The application of coarse-grained molecular dynamics to the evaluation of liposome physical stability[J]. AAPS Pharm Sci Tech,2020,21(5):2-8.
[3]	Shao X,Wei X,Zhang S,et al. Effects of micro-environmental pH of liposome on chemical stability of loaded drug[J]. Nanoscale Research Letters,2017,12(1):2-8. doi: 10.1186/s11671-016-1772-3
[4]	余荧蓝,郑智元,伊宸辰,等. 青蒿素长循环脂质体的制备及体外性质评价[J]. 药学学报,2018,53(6):1002-1008.
[5]	王甫,李芳,李艳春,等. 聚乙二醇6000合成工艺的热危险性[J]. 爆破器材,2021,50(3):1-6. doi: 10.3969/j.issn.1001-8352.2021.03.001
[6]	Harandi A M. Systems analysis of human vaccine adjuvants[J]. Seminars in Immunology,2018,39(1):30-34.
[7]	Yoshizawa Y,Kono Y,Ogawara K,et al. PEG liposomalization of paclitaxel improved its in vivo disposition and anti-tumor efficacy[J]. International Journal of Pharmaceutics,2011,412(1-2):132-141. doi: 10.1016/j.ijpharm.2011.04.008
[8]	Ibaraki H,Takeda A,Arima N,et al. In vivo fluorescence imaging of passive inflammation site accumulation of liposomes via intravenous administration focused on their surface charge and PEG modification[J]. Pharmaceutics,2021,13(1):104. doi: 10.3390/pharmaceutics13010104
[9]	张勇,李娟,肖波,等. PEG修饰水飞蓟素脂质体的制备及体外释放研究[J]. 药学与临床研究,2010,18(3):239-242. doi: 10.3969/j.issn.1673-7806.2010.03.012
[10]	陈涛,王昭,傅经国,商澎. 聚乙二醇修饰脂质体研究[J]. 世界最新医学信息文摘,2003,2(5):801-805.
[11]	鲁卫东,林意菊,马波,等. 流感疫苗脂质体干粉的稳定性[J]. 中国药科大学学报,2010,41(4):360-362. doi: 10.11665/j.issn.1000-5048.20100413
[12]	Yokoe J,Sakuragi S,Yamamoto K,et al. Albumin-conjugated PEG liposome enhances tumor distribution of liposomal doxorubicin in rats[J]. International Journal of Pharmaceutics,2008,353(1-2):28-34. doi: 10.1016/j.ijpharm.2007.11.008
[13]	鲁卫东,林意菊,代云波,等. 流感疫苗脂质体冻干粉的制备及其免疫原性[J]. 中国药科大学学报,2009,40(3):218-221. doi: 10.3321/j.issn:1000-5048.2009.03.006
[14]	乔建斌,陈娜,李强,等. 流感疫苗壳聚糖脂质体的制备工艺[J]. 昆明医科大学学报,2014,35(4):38-40.
[15]	国家药典委员会. 中华人民共和国药典[M]. 三部, 北京: 中国医药科技出版社, 2020: 1088
[16]	吴晶晶,吴楠,岳华,等. 壳聚糖季铵盐微球作为注射疫苗佐剂的安全性评价[J]. 过程工程学报,2016,16(4):647-653. doi: 10.12034/j.issn.1009-606X.215427
[17]	高文慧,陈美华,曹雨酥,等. 长循环吗啡脂质体的制备及质量评价[J]. 中国药师,2020,23(4):643-647. doi: 10.3969/j.issn.1008-049X.2020.04.008
[18]	王宏宇,古金元,彭涛,等. 脂质体作为免疫增强剂在动物疾病防治方面的应用现状[J]. 动物医学进展,2019,40(5):106-109.
[19]	Sadzuka Y,Kishi K,Hirota S,et al. Effect of Polyethyleneglycol (PEG) chain on cell uptake of PEG-modified liposomes[J]. Journal of Liposome Research,2003,13(2):157-172.
[20]	Zhao Y, Zheng H, Wang X, et al. Preparation and biological property evaluation of novel cationic lipid-based liposomes for efficient gene delivery[J]. AAPS PharmSciTech, 2021, 22（1）: DOI: 10.1208/s12249-020-01868-w.
[21]	李雨欣,陈琳,李娟,等. 聚乙二醇修饰白藜芦醇脂质体的特性及其抗肿瘤实验研究[J]. 陕西师范大学学报(自然科学版),2020,48(1):92-98.
[22]	Dan N. Effect of liposome charge and PEG polymer layer thickness on cell–liposome electrostatic interactions[J]. Biochimica et Biophysica Acta. Biomembranes,2002,1564(2):343-348. doi: 10.1016/S0005-2736(02)00468-6
[23]	Lee J S,Hwang S Y,Lee E K. Imaging-based analysis of liposome internalization to macrophage cells:Effects of liposome size and surface modification with PEG moiety[J]. Colloids and Surfaces B:Biointerfaces,2015,136(2):786-790.
[24]	张壮丽,汪婉莹,赵璐丹,等. 聚乙二醇修饰鱼腥草挥发油脂质体的制备与评价[J]. 中药材,2020,43(11):2763-2767.
[25]	Pasquale A,Preiss S,Silva F,et al. Vaccine adjuvants:from 1920 to 2015 and beyond[J]. Vaccines,2015,3(2):320-343.
[26]	Yan Zhuang A Y M A C. PEGylated cationic liposomes robustly augment vaccine-induced immune responses:Role of lymphatic trafficking and biodistribution[J]. Journal of Controlled Release,2012(159):135-142.

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