Preparation and Stability of Influenza Vaccine Liposomes Modified by PEG6000

Piao LI; Xiaolin LIU; Ai YANG; Qian ZHANG; Weidong LU

doi:10.12259/j.issn.2095-610X.S20220307

Volume 43 Issue 3

Mar. 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(3): 32-36

Piao LI, Xiaolin LIU, Ai YANG, Qian ZHANG, Weidong LU. Preparation and Stability of Influenza Vaccine Liposomes Modified by PEG6000[J]. Journal of Kunming Medical University, 2022, 43(3): 32-36. doi: 10.12259/j.issn.2095-610X.S20220307

Citation:

Piao LI, Xiaolin LIU, Ai YANG, Qian ZHANG, Weidong LU. Preparation and Stability of Influenza Vaccine Liposomes Modified by PEG6000[J]. Journal of Kunming Medical University, 2022, 43(3): 32-36. doi: 10.12259/j.issn.2095-610X.S20220307

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Preparation and Stability of Influenza Vaccine Liposomes Modified by PEG6000

doi: 10.12259/j.issn.2095-610X.S20220307

School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-01-12
Available Online: 2022-02-18
Publish Date: 2022-03-22

Abstract

Abstract

Objective To screen out the best method of PEG6000 modified liposomes for influenza vaccine and evaluate its stability. Methods After 7 d of immunization, the optimal molar percentage of PEG6000 and the optimal preparation method were assumed by MTT method. The PEG-modified influenza vaccine liposomes were prepared by freeze-thaw freeze-drying method. The samples were stored at different temperatures （4 ℃, 25±2 ℃, 37±2 ℃）, and the encapsulation rate and thymus index and antibody titer were measured at a fixed time. So the physical and biological stability of the samples were investigated. Results The MTT experiment showed that PEG6000 was the best dosage when the molar percentage of phospholipid was 4% （P < 0.01）, and the best process was post-modification method.The entrapment rate of PEG6000 modified liposome for influenza vaccine was still higher than 80% when stocked at 37±2 ℃ for 6 months.At 25±2 ℃ for 3 months, the antibody titer was more than 4, indicating that the antibody still had nice immune effection. Conclusions PEG6000 best dosage of phospholipid when the molar percentage of PEG6000 is 4%, and post-modification method is the best preparation process. PEG6000 liposome has nice physical stability and biological stability.
- Liposomes,
- PEG6000,
- Influenza vaccine,
- Stability

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References(26)

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