Clinical Analysis on the Detection of Human Cytomegalovirus DNA in 2403 Samples of Different Types

Jinyu ZHOU; Juding GUO; Yuming WANG; Qian ZHANG

doi:10.12259/j.issn.2095-610X.S20220326

Volume 43 Issue 3

Mar. 2022

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Jinyu ZHOU, Juding GUO, Yuming WANG, Qian ZHANG. Clinical Analysis on the Detection of Human Cytomegalovirus DNA in 2403 Samples of Different Types[J]. Journal of Kunming Medical University, 2022, 43(3): 94-97. doi: 10.12259/j.issn.2095-610X.S20220326

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Jinyu ZHOU, Juding GUO, Yuming WANG, Qian ZHANG. Clinical Analysis on the Detection of Human Cytomegalovirus DNA in 2403 Samples of Different Types[J]. Journal of Kunming Medical University, 2022, 43(3): 94-97. doi: 10.12259/j.issn.2095-610X.S20220326

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Clinical Analysis on the Detection of Human Cytomegalovirus DNA in 2403 Samples of Different Types

doi: 10.12259/j.issn.2095-610X.S20220326

1.
Dept. of Clinical Laboratory, Kunming City Maternal and Child Health Hospital，Kunming Yunnan 650031
2.
Dept. of Clinical Laboratory，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

Received Date: 2022-02-12
Available Online: 2022-02-18
Publish Date: 2022-03-22

Abstract

Abstract

Objective To analyze the detection of human cytomegalovirus （HCMV） DNA in different types of samples from pregnant women and infants. Methods From May 2021 to October, a total of 2403 clinical samples were collected. Fluorescence quantitative PCR to test the HCMV-DNA, and we analyzed positive rate of HCMV-DNA in different samples sources and types retrospectively. Results Among different types of samples, the positive rate of HCMV-DNA in maternal milk was 31.70%, and that in urine was 0.85%. The positive rate of maternal milk and urine samples increased with the increase of age, and the difference was statistically significant （P < 0.001）. Among 221 urine samples from infants as well as their maternal milk samples, the positive rate of maternal milk samples was higher than that of urine or serum. When the maternal milk samples were negative, 0.58% of the children’ s urine samples were positive; When the maternal milk samples were positive, the children’ s urine were negative. Conclusions The positive rate of HCMV-DNA in maternal milk samples is significantly higher than that in other types of samples. Therefore, for pregnant women or infants with suspected infected HCMV, it’ s recommended to collect milk samples for the test. However, HCMV-DNA detected in maternal milk can’ t fully reflect the detection of infant body fluid samples. As age goes on, the positive rate of infant urine will increase. It is necessary to collect fluid samples at different periods for the test, and analyze comprehensively the test results.
- Human cytomegalovirus （HCMV）,
- Fluorescence quantitative PCR,
- Specimens of body fluid

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References

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