Clinical Features of Tuberculosis with Negative T Cell Spot Test in Peripheral Blood

Rong WAN; Guangmei LI; Meng MA; Minghong LAI; Cai LIU; Mingwu LI

doi:10.12259/j.issn.2095-610X.S20220420

Volume 43 Issue 4

Apr. 2022

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Rong WAN, Guangmei LI, Meng MA, Minghong LAI, Cai LIU, Mingwu LI. Clinical Features of Tuberculosis with Negative T Cell Spot Test in Peripheral Blood[J]. Journal of Kunming Medical University, 2022, 43(4): 132-136. doi: 10.12259/j.issn.2095-610X.S20220420

Citation:

Rong WAN, Guangmei LI, Meng MA, Minghong LAI, Cai LIU, Mingwu LI. Clinical Features of Tuberculosis with Negative T Cell Spot Test in Peripheral Blood[J]. Journal of Kunming Medical University, 2022, 43(4): 132-136. doi: 10.12259/j.issn.2095-610X.S20220420

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Clinical Features of Tuberculosis with Negative T Cell Spot Test in Peripheral Blood

doi: 10.12259/j.issn.2095-610X.S20220420

Dept. of Tuberculosis，The 3rd People’s Hospital of Kunming，Kunming Yunnan 650041， China

Received Date: 2022-02-21
Available Online: 2022-03-15
Publish Date: 2022-04-25

Abstract

Abstract

Objective To analyze the clinical features of active tuberculosis cases with negative T cell spot assay （T-SPOT.TB） in peripheral blood. Methods A total of 83 active tuberculosis patients admitted to the Second Department of Tuberculosis in Kunming Third People’ s Hospital from January to December 2019 who tested negative T-SPOT.TB were selected as the study subjects; another 82 active tuberculosis patients who tested positive T-SPOT.TB were selected as the control. The clinical characteristics of the two groups were compared, and the positive rates of several conventional experimental detection methods were compared. Results There was no difference in age and gender between the T-spot. TB negative group and the T-spot. TB positive group （P > 0.05）, however, there were more patients over 60 years old in the T-spot TB negative group （22.9%）, but there was no difference, in 2 groups （P > 0.05）. There was no difference in the comorbidity of the 2 groups, and diabetes was more common （ P > 0.05）. The incidence of extrapulmonary tuberculosis was about half in both groups; tuberculosis pleurisy was more common in the T-SPOT.TB negative group, and tracheal tuberculosis was more common in the positive group. Cellular immune related examination showed nearly 90% of patients have low cellular immune function. All cases were routinely tested for acid-fast bacilli smear, TB-DNA, XpertMtb/RIF and TB culture. In the 2 groups, the positive rate of XpertMtb/RIF was the highest （77.1% and 64.6%, respectively）, which was significantly higher than the other 3 detection methods. There was statistical difference （P < 0.001）. Conclusion Old age, complicating extrapulmonary tuberculosis, and low immune function may be the causes of T-SPOt-TB negative in active tuberculosis. XpertMtb/RIF test can be used for rapid early diagnosis of TB.
- Active,
- Tuberculosis,
- Tuberculosis infection,
- T cell spot assay,
- Negative

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