EGCG Improves Myocardial Fibrosis Induced by High Fat and High Sugar Diet in Obese Rats by Inhibiting TGF-β1/Smads Signaling Pathway

Cong LIU; Guishuai WU; Rui PU; Shude LI; Jianping TAO; Renfa ZHANG

doi:10.12259/j.issn.2095-610X.S20220506

Volume 43 Issue 5

May 2022

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Cong LIU, Guishuai WU, Rui PU, Shude LI, Jianping TAO, Renfa ZHANG. EGCG Improves Myocardial Fibrosis Induced by High Fat and High Sugar Diet in Obese Rats by Inhibiting TGF-β1/Smads Signaling Pathway[J]. Journal of Kunming Medical University, 2022, 43(5): 18-26. doi: 10.12259/j.issn.2095-610X.S20220506

Citation:

Cong LIU, Guishuai WU, Rui PU, Shude LI, Jianping TAO, Renfa ZHANG. EGCG Improves Myocardial Fibrosis Induced by High Fat and High Sugar Diet in Obese Rats by Inhibiting TGF-β1/Smads Signaling Pathway[J]. Journal of Kunming Medical University, 2022, 43(5): 18-26. doi: 10.12259/j.issn.2095-610X.S20220506

Citation:

Cong LIU, Guishuai WU, Rui PU, Shude LI, Jianping TAO, Renfa ZHANG. EGCG Improves Myocardial Fibrosis Induced by High Fat and High Sugar Diet in Obese Rats by Inhibiting TGF-β1/Smads Signaling Pathway[J]. Journal of Kunming Medical University, 2022, 43(5): 18-26. doi: 10.12259/j.issn.2095-610X.S20220506

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EGCG Improves Myocardial Fibrosis Induced by High Fat and High Sugar Diet in Obese Rats by Inhibiting TGF-β1/Smads Signaling Pathway

doi: 10.12259/j.issn.2095-610X.S20220506

1.
Dept. of Anesthesiology，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101
2.
School of Basic Medicine, Kunming Medical University
3.
Dept. of Biochemistry and Molecular Biology，School of Basic Medicine
4.
Dept. of Physical Education，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-02-18
Available Online: 2022-05-07
Publish Date: 2022-05-27

Abstract

Abstract

Objective To investigate the effects of epigallocatechin gallate （EGCG） on myocardial fibrosis induced by high fat and high sugar diet in obese rats and to explore whether EGCG can reduce the extent of myocardial fibrosis through TGF-β1/Smads signaling pathway. Methods A obese rat model was formed by long-term high fat and high sugar diet. After 4 weeks of EGCG intragastric administration daily in obese rats, the body weight of every group rats was examined. The extent of myocardial fibrosis was detected by Masson staining. The contents of fasting blood glucose （FBG）, triglyceride （TG）, total cholesterol （TC）, malondialdehyde （MDA） and glutathione （GSH） in serum were determined by biochemical methods. Western blotting was used to detect the expression levels of Collagen Ⅰ, Collagen Ⅲ, TGF-β1, Smad2/3, p-Smad2/3 and Smad7 in heart tissues of the rats in each group. Results The body weight of the model group rats was incremental than those in the control group and the body weight of EGCG treatment was lower. The extent of myocardial fibrosis was more severe in the model group to the control group, and decreased after EGCG treatment. The contents of FBG, TC, TG and MDA in model group were higher than those in the control group, but decreased after EGCG treatment. The activity of GSH in the model group was lower than that in the control group, but increased after EGCG treatment. Collagen Ⅰ, Collagen Ⅲ, TGF-β1, Smad2/3 and p-Smad2/3 were increased in the model group, but decreased after EGCG treatment. Smad7 protein expression in the model group was lower than that in the control group, but increased after EGCG treatment. Conclusion EGCG can improve the lipid metabolism disorder and reduce MF degree in obese rats, which can be related to TGF-β1/Smads signaling pathway mediated by oxidative stress.
- Epigallocatechin gallate,
- Obesity,
- Myocardial fibrosis,
- TGF-β1/Smads signaling pathway

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