Studies on the Preparation Method of Influenza Vaccine Liposome and the Effect of Particle Size on Immunogenicity

Xiaolin LIU; Qingxin XIE; Piao LI; Qian ZHANG; Ai YANG; Weidong LU

doi:10.12259/j.issn.2095-610X.S20220508

Volume 43 Issue 5

May 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(5): 7-11

Xiaolin LIU, Qingxin XIE, Piao LI, Qian ZHANG, Ai YANG, Weidong LU. Studies on the Preparation Method of Influenza Vaccine Liposome and the Effect of Particle Size on Immunogenicity[J]. Journal of Kunming Medical University, 2022, 43(5): 7-11. doi: 10.12259/j.issn.2095-610X.S20220508

Citation:

Xiaolin LIU, Qingxin XIE, Piao LI, Qian ZHANG, Ai YANG, Weidong LU. Studies on the Preparation Method of Influenza Vaccine Liposome and the Effect of Particle Size on Immunogenicity[J]. Journal of Kunming Medical University, 2022, 43(5): 7-11. doi: 10.12259/j.issn.2095-610X.S20220508

Citation:

Xiaolin LIU, Qingxin XIE, Piao LI, Qian ZHANG, Ai YANG, Weidong LU. Studies on the Preparation Method of Influenza Vaccine Liposome and the Effect of Particle Size on Immunogenicity[J]. Journal of Kunming Medical University, 2022, 43(5): 7-11. doi: 10.12259/j.issn.2095-610X.S20220508

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Studies on the Preparation Method of Influenza Vaccine Liposome and the Effect of Particle Size on Immunogenicity

doi: 10.12259/j.issn.2095-610X.S20220508

School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2022-01-12
Available Online: 2022-05-12
Publish Date: 2022-05-27

Abstract

Abstract

Objective （1） To prepare the tetravalent influenza vaccine liposome lyophilized powder with the film dispersion method and freeze-thaw lyophilization method, and to screen the best preparation method by investigating its particle size, entrapment efficiency and immunogenicity. （2） To prepare the influenza vaccine liposomes with different particle sizes by using the best preparation method, and to screen the best particle size by comparing the encapsulation efficiency and immunogenicity. Methods Lowry method was used to determine liposome entrapment rate. At given days of mice abdominal immunization, the cellular immunogenicity was conducted by spleen lymphocyte proliferation assay with stimulation index （SI） as indexes. The hemagglutination inhibition test was used to evaluate the humoral immunogenicity after the mice abdominal immunization. Results Freeze-thaw and freeze-drying method was the best method to prepare influenza vaccine liposome freeze-dried powder. Four kinds of liposome freeze-dried powder with average particle size of 3.7 µm, 2.0 µm, 1.0 µm and 0.45 µm were prepared by the best method. In one immune cycle, each experimental group could significantly stimulate the proliferation of spleen lymphocytes and produce the cellular immunity. There was significant difference between 3.7 µm group and other groups（P < 0.05）. The titer ratio of antibody after and before the immunization was always ≥4, and the 3.7 µm group was higher than the other groups, indicating that it could produce the strong humoral immunity. Conclusion The tetravalent influenza vaccine liposomes prepared by freeze-thaw freeze-dried method can produce the strong immunogenicity, and the liposomes with the particle size of 3.7µm have the best immuno-enhancement effect.
- Liposomes,
- Influenza vaccine,
- Particle size,
- Immunogenicity

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References(15)

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