Studies on Moisture-absorption-retention and Antioxidant Activity of <i>Panax Notoginseng</i> Polysaccharides in Vitro

Xiaoping WU; Yuling DAI; Ling ZHANG; Hong WANG; Qiong ZOU; Jinji WANG; Yanhong LIU; Tong CHEN

doi:10.12259/j.issn.2095-610X.S20220514

Volume 43 Issue 5

May 2022

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Xiaoping WU, Yuling DAI, Ling ZHANG, Hong WANG, Qiong ZOU, Jinji WANG, Yanhong LIU, Tong CHEN. Studies on Moisture-absorption-retention and Antioxidant Activity of Panax Notoginseng Polysaccharides in Vitro[J]. Journal of Kunming Medical University, 2022, 43(5): 1-6. doi: 10.12259/j.issn.2095-610X.S20220514

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Xiaoping WU, Yuling DAI, Ling ZHANG, Hong WANG, Qiong ZOU, Jinji WANG, Yanhong LIU, Tong CHEN. Studies on Moisture-absorption-retention and Antioxidant Activity of Panax Notoginseng Polysaccharides in Vitro[J]. Journal of Kunming Medical University, 2022, 43(5): 1-6. doi: 10.12259/j.issn.2095-610X.S20220514

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Studies on Moisture-absorption-retention and Antioxidant Activity of Panax Notoginseng Polysaccharides in Vitro

doi: 10.12259/j.issn.2095-610X.S20220514

1.
School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunnan 650500
2.
Dept. of Gerontology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
3.
Yunnan Maternal and Child Health Hospital，Kunming Yunnan 650051，China

Received Date: 2022-01-15
Available Online: 2022-05-06
Publish Date: 2022-05-27

Abstract

Abstract

Objective To investigate hygroscopic, moisturizing properties and antioxidant activities in vitro by extracting and separating Panax notoginseng polysaccharides from industrial medicinal residues extracted from Panax notoginseng total saponins. Methods The crude polysaccharide from Panax notoginseng （CPPN） was extracted by water extraction and alcohol precipitation with industrial Panax notoginseng residues andCPPN was separated by DEAE Sepharose Fast Flow. Compared with glycerol and sodium alginate, the hygroscopic properties of Panax notoginseng polysaccharide under the relative humidity （RH） of 43% and 81% and the moisturizing properties under dry silica gel environment were measured. The antioxidant activity of Panax notoginseng polysaccharide in vitro was investigated by DPPH radical, ABTS⁺ radical and hydroxyl radical scavenging test. Results CPPN was separated by DEAE Sepharose Fast Flow to obtain a neutral polysaccharide from Panax notoginseng （NPPN） and three acidic polysaccharides from Panax notoginseng （APPN）, named as NPPN, APPNⅠ, APPNII, APPNⅢ, respectively. The rates were 27.68%, 11.89%, 15.41% and 21.04%, respectively. When the RH was 43%, the moisture absorption rate was glycerol>APPNⅢ>sodium alginate>CPPN>APPNⅡ>APPNⅠ>NPPN. When the RH was 81%, the moisture absorption rate was glycerol >APPNⅢ>sodium alginate>APPNⅡ>APPNⅠ>CPPN>NPPN. APPNⅢ had better hygroscopicity than sodium alginate. In dry silica gel environment, the moisturizing rate was APPNⅢ>sodium alginate>CPPN>APPNⅡ>glycerin>APPNⅠ>NPPN. The scavenging sequence for DPPH radical and ABTS radical was CPPN>APPNⅠ>APPNⅢ>APPNⅡ>NPPN. The scavenging sequence for hydroxyl radical was CPPN>APPNⅢ>APPNⅡ>APPNⅠ>NPPN. Conclusion CPPN and its isolated polysaccharides have certain moisture absorption, moisturizing properties and antioxidant activity in vitro. Among them, APPN Ⅲ has the strongest moisture absorption and moisturizing performance, and CPPN has the strongest antioxidant activity in vitro.
- Panax notoginseng polysaccharides,
- Moisture aborsption,
- Moisture retention,
- Antioxidant

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