Volume 43 Issue 5
May  2022
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Li ZHANG, Qingcen WANG, Luohui ZHOU, Juan YANG, Hong WANG. Luteolin Inhibits HASMCs Migration by Endoplasmic Reticulum Stress[J]. Journal of Kunming Medical University, 2022, 43(5): 12-17. doi: 10.12259/j.issn.2095-610X.S20220518
Citation: Li ZHANG, Qingcen WANG, Luohui ZHOU, Juan YANG, Hong WANG. Luteolin Inhibits HASMCs Migration by Endoplasmic Reticulum Stress[J]. Journal of Kunming Medical University, 2022, 43(5): 12-17. doi: 10.12259/j.issn.2095-610X.S20220518

Luteolin Inhibits HASMCs Migration by Endoplasmic Reticulum Stress

doi: 10.12259/j.issn.2095-610X.S20220518
  • Received Date: 2021-11-05
    Available Online: 2022-05-12
  • Publish Date: 2022-05-27
  •   Objective  To investigate the regulatory effect and mechanism of luteolin on the migration of human aortic smooth muscle cells (HASMCs).  Methods  HASMCs were cultured in vitro and divided into the blank control group (without any treatment, BC), the negative control group (with the same amount of irrelevant reagent, NC), the luteolin group (25 μmol/L treatment, LUT) and the luteolin+4-PBA treatment group (with the same amout of luteolin added after 4-PBA pretreatment, 4-PBA). Cell scratch test was used to detect the HASMCs migration after 12hs. The expression level of BIP and CHOP about endoplasmic reticulum stress were detected by RT-qPCR and Western blot.   Results   (1) Compared to the BC and NC groups, the cell migration level in the LUT group was significantly reduced, and the expression levels of BIP and CHOP were significantly increased. (2) Compared with the LUT group, the cell migration level of the luteolin+4-PBA co-teatment group increased, while the expression levels of BIP and CHOP decreased.   Conclusion  Luteolin inhibits HASMCs migration by promoting the endoplasmic reticulum stress.
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  • [1]
    Ye J,Wang Y,Wang Z,et al. Roles and mechanisms of interleukin-12 family members in cardiovascular diseases:Opportunities and challenges[J]. Front Pharmacol,2020,11(3):129.
    [2]
    Papoutsis K,Kapelouzou A,Georgiopoulos G,et al. Tissue-specific relaxin-2 is differentially associated with the presence/size of an arterial aneurysm and the severity of atherosclerotic disease in humans[J]. Acta Pharmacol Sin,2020,41(6):745-752. doi: 10.1038/s41401-019-0350-5
    [3]
    Luo Z,Deng H,Fang Z,et al. Ligustilide inhibited rat vascular smooth muscle cells migration via c-Myc/MMP2 and ROCK/JNK signaling pathway[J]. J Food Sci,2019,84(12):3573-3583. doi: 10.1111/1750-3841.14936
    [4]
    Da C F,Waczuk E P,Duarte A E,et al. Cytotoxic and antioxidative potentials of ethanolic extract of Eugenia uniflora L. (Myrtaceae) leaves on human blood cells[J]. Biomed Pharmacother,2016,84(12):614-621.
    [5]
    Yao Y,Rao C,Zheng G,et al. Luteolin suppresses colorectal cancer cell metastasis via regulation of the miR384/pleiotrophin axis[J]. Oncol Rep,2019,42(1):131-141.
    [6]
    Huang L,Jin K,Lan H. Luteolin inhibits cell cycle progression and induces apoptosis of breast cancer cells through downregulation of human telomerase reverse transcriptase[J]. Oncol Lett,2019,17(4):3842-3850.
    [7]
    Hetz C,Chevet E,Harding H P. Targeting the unfolded protein response in disease[J]. Nat Rev Drug Discov,2013,12(9):703-719.
    [8]
    Brooks R L,Dixon A M. Revealing the mechanism of protein-lipid interactions for a putative membrane curvature sensor in plant endoplasmic reticulum[J]. Biochim Biophys Acta Biomembr,2020,1862(3):183160. doi: 10.1016/j.bbamem.2019.183160
    [9]
    Balsa E,Soustek M S,Thomas A,et al. ER and nutrient stress promote assembly of respiratory chain supercomplexes through the PERK-eIF2alpha axis[J]. Mol Cell,2019,74(5):877-890. doi: 10.1016/j.molcel.2019.03.031
    [10]
    Redza-Dutordoir M,Averill-Bates D A. Activation of apoptosis signalling pathways by reactive oxygen species[J]. Biochim Biophys Acta,2016,1863(12):2977-2992. doi: 10.1016/j.bbamcr.2016.09.012
    [11]
    Hsuan C F,Hsu H F,Tseng W K,et al. Glossogyne tenuifolia extract inhibits TNF-alpha-induced expression of adhesion molecules in human umbilical vein endothelial cells via blocking the NF-kB signaling pathway[J]. Molecules,2015,20(9):16908-16923. doi: 10.3390/molecules200916908
    [12]
    Zhang B C,Li Z,Xu W,et al. Luteolin alleviates NLRP3 inflammasome activation and directs macrophage polarization in lipopolysaccharide-stimulated RAW264.7 cells[J]. Am J Transl Res,2018,10(1):265-273.
    [13]
    Hattori S,Kamiya T,Hara H,et al. CoCl2 decreases EC-SOD expression through histone deacetylation in COS7 cells[J]. Biol Pharm Bull,2016,39(12):2036-2041. doi: 10.1248/bpb.b16-00551
    [14]
    Nekohashi M,Ogawa M,Ogihara T,et al. Luteolin and quercetin affect the cholesterol absorption mediated by epithelial cholesterol transporter niemann-pick c1-like 1 in caco-2 cells and rats[J]. PLoS One,2014,9(5):e97901. doi: 10.1371/journal.pone.0097901
    [15]
    Huang C,He C,Ruan P,et al. TSPYL5 activates endoplasmic reticulum stress to inhibit cell proliferation,migration and invasion in colorectal cancer[J]. Oncol Rep,2020,44(2):449-456. doi: 10.3892/or.2020.7639
    [16]
    Fu Y F,Liu X,Gao M,et al. Endoplasmic reticulum stress induces autophagy and apoptosis while inhibiting proliferation and drug resistance in multiple myeloma through the PI3K/Akt/mTOR signaling pathway[J]. Oncotarget,2017,8(37):61093-61106. doi: 10.18632/oncotarget.17862
    [17]
    Zhang S R,Zhang X C,Liang J F,et al. Chalcomoracin inhibits cell proliferation and increases sensitivity to radiotherapy in human non-small cell lung cancer cells via inducing endoplasmic reticulum stress-mediated paraptosis[J]. Acta Pharmacol Sin,2020,41(6):825-834. doi: 10.1038/s41401-019-0351-4
    [18]
    Pu H,Qian Q,Wang F,et al. Schizandrin A induces the apoptosis and suppresses the proliferation,invasion and migration of gastric cancer cells by activating endoplasmic reticulum stress[J]. Mol Med Rep,2021,24(5):787. doi: 10.3892/mmr.2021.12427
    [19]
    Park S H,Park H S,Lee J H,et al. Induction of endoplasmic reticulum stress-mediated apoptosis and non-canonical autophagy by luteolin in NCI-H460 lung carcinoma cells[J]. Food Chem Toxicol,2013,56(6):100-109.
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