Long Non-coding RNA-p21 Regulates the MicroRNA-9 / Sirtuin-1 Signaling Pathway to Reverse Oxaliplatin Resistance in Colorectal Cancer Cells

Liju ZHANG; Xiaoming JIANG; Changxian CHEN; Xi WU; Zhenyong ZHANG; Weijun LIU

doi:10.12259/j.issn.2095-610X.S20220519

Volume 43 Issue 5

May 2022

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Article Navigation > Journal of Kunming Medical University > 2022 > 43(5): 27-32

Liju ZHANG, Xiaoming JIANG, Changxian CHEN, Xi WU, Zhenyong ZHANG, Weijun LIU. Long Non-coding RNA-p21 Regulates the MicroRNA-9 / Sirtuin-1 Signaling Pathway to Reverse Oxaliplatin Resistance in Colorectal Cancer Cells[J]. Journal of Kunming Medical University, 2022, 43(5): 27-32. doi: 10.12259/j.issn.2095-610X.S20220519

Citation:

Liju ZHANG, Xiaoming JIANG, Changxian CHEN, Xi WU, Zhenyong ZHANG, Weijun LIU. Long Non-coding RNA-p21 Regulates the MicroRNA-9 / Sirtuin-1 Signaling Pathway to Reverse Oxaliplatin Resistance in Colorectal Cancer Cells[J]. Journal of Kunming Medical University, 2022, 43(5): 27-32. doi: 10.12259/j.issn.2095-610X.S20220519

Citation:

Liju ZHANG, Xiaoming JIANG, Changxian CHEN, Xi WU, Zhenyong ZHANG, Weijun LIU. Long Non-coding RNA-p21 Regulates the MicroRNA-9 / Sirtuin-1 Signaling Pathway to Reverse Oxaliplatin Resistance in Colorectal Cancer Cells[J]. Journal of Kunming Medical University, 2022, 43(5): 27-32. doi: 10.12259/j.issn.2095-610X.S20220519

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Long Non-coding RNA-p21 Regulates the MicroRNA-9 / Sirtuin-1 Signaling Pathway to Reverse Oxaliplatin Resistance in Colorectal Cancer Cells

doi: 10.12259/j.issn.2095-610X.S20220519

1.
Faculty of Medicine，Yunnan University，Kunming Yunnan 650091
2.
Dept. of Anorectal Diseases，The 1st People’s Hospital of Yunnan Province，The Affiliated Hospital to Kunming University of Science and Technology，Kunming Yunnan 650032
3.
Faculty of Medicine，Kunming University of Science and Technology，Kunming Yunnan 650031，China

Received Date: 2022-01-01
Available Online: 2022-05-12
Publish Date: 2022-05-27

Abstract

Abstract

Objectives To discuss the molecular mechanism of long non－coding RNA(lncRNA）-P21 regulating （miR-9） /Sirtuin-1(SIRT1） signaling pathway in reversing colorectal cancer （CRC） cell autophagy and drug resistance. Methods Real-time fluorescent quantitative PCR （RT-qPCR） was employed to assess the expression level of LncRNA-p21 in cell lines. Western blotting was performed to measure the expression of SIRT1, miR-9 and autophagy related proteins in CRC cell. Clone formation assay was used to detect the cell activity. Results (1） LncRNA-p21 was highly expressed in oxaliplatin - resistant CRC cells HCT116 and SW620. （2） The knockdown of LncRNA-p21was able to inhibit autophagy and improve the chemotherapy sensitivity of HCT116 cells to oxaliplatin. （3） After LncRNA-p21 was knocked down, the expression of miR-9 was up-regulated, while the overexpression of miR-9 could enhance the chemotherapy sensitivity of HCT116 cells to oxaliplatin. Meanwhile, the expression of SIRT1 was regulated by LncRNA-p21 and miR-9. Conclusion CRC resistance to chemotherapy drug oxaliplatin may be caused by autophagy induced by LncRNA-P21, which may be realized through the regulation of the miR-9 /SIRT1 signaling pathway by LncRNA-P21.
- Oxaliplatin,
- LncRNA-p21,
- miR-9/SIRT1 signaling pathways,
- Tolerance,
- Autophagy,
- Colorectal cancer

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References(15)

References

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