The Expression and Clinical Significance of Non-coding RNA in Cervical Cancer

Fangyuan GUO; Wenliang LI; Fan XU; Xiangqun WEI

doi:10.12259/j.issn.2095-610X.S20220527

Volume 43 Issue 5

May 2022

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Fangyuan GUO, Wenliang LI, Fan XU, Xiangqun WEI. The Expression and Clinical Significance of Non-coding RNA in Cervical Cancer[J]. Journal of Kunming Medical University, 2022, 43(5): 108-116. doi: 10.12259/j.issn.2095-610X.S20220527

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Fangyuan GUO, Wenliang LI, Fan XU, Xiangqun WEI. The Expression and Clinical Significance of Non-coding RNA in Cervical Cancer[J]. Journal of Kunming Medical University, 2022, 43(5): 108-116. doi: 10.12259/j.issn.2095-610X.S20220527

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The Expression and Clinical Significance of Non-coding RNA in Cervical Cancer

doi: 10.12259/j.issn.2095-610X.S20220527

1.
Dept.of Gynaecology，The Affiliated Hospital of Yunnan University， Kunming Yunnan 650021
2.
Dept. of Gynaecology，The 3rd Affiliated Hospital of Kunming Medical University， Kunming Yunnan 650118，China

Received Date: 2022-01-01
Available Online: 2022-05-12
Publish Date: 2022-05-27

Abstract

Abstract

Objective To analyze the expression of long non-coding RNA （LINC00982） and microrNA （hsa-miR-1246） in cervical cancer tissues, so as to provide a new biomarker for the diagnosis of cervical cancer and a potential and effective therapeutic target for its treatment. Methods The normal cervical tissue samples （as the control group） were collected from 21 patients undergoing the total hysterectomy for benign lesions such as uterine fibroids and the cervical cancer tissue samples （as the experimental group） were collected from 39 patients diagnosed as cervical cancer by pathology. All the patients above were admitted to the Affiliated Hospital of Yunnan University from January 2021 to October 2021. The expressions of LINC00982 and miR-1246 were detected and compared between these two groups by real-time fluorescence quantitative PCR （RT-QPCR）. Results （1） Compared with the normal human cervical tissues, the expression of LINC00982-1/2 was down-regulated in cervical cancer tissues （P < 0.05）, while the expression of hsa-miR-1246 was up-regulated （P < 0.05）; （2）The expression levels of LINC00982-1/2 and miR-1246 in highly differentiated cervical cancer tissues were lower than those in medium and low differentiated cervical cancer tissues （P < 0.05）; （3） The expression levels of LINC00982-1/2 and hsa-miR-1246 in stage Ⅲ/Ⅳ of the cervical carcinoma were higher than those in stage Ⅰ/Ⅱ （P < 0.05）. （4） The expression level of LINC00982-1 in adenocarcinoma tissues was lower than that in squamous cell carcinoma （P < 0.05）, but there was no significant difference between adenocarcinoma and squamous cell carcinoma （P > 0.05）, while the expression of hsa-mir-1246 was higher than that in squamous cell carcinoma （P < 0.05）; （5） The expression of LINC00982-1/2 decreased with the increase of SCCA （P < 0.05）, while the expression of hsa-miR-1246 increased with the increase of SCCA; （6） The expression levels of LINC00982-1/2 and hsa-miR-1246 in tissues with the tumor diameter > 4 cm were higher than those with tumor diameter ≤4 cm. Conclusion LINC00982 is low expressed in cervical cancer tissues while hsa-miR-1246 is highly expressed in cervical cancer tissues. Furthermore, the expressions of LINC00982-1/2 and hsa-miR-1246 in cervical cancer tissues are closely related to the degree of tissue differentiation, pathological stage, pathological type, SCCA level and tumor diameter.
- Cervical cancer,
- Long non-coding RNA,
- micro RNA

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References(15)

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