Correlation between CD3<sup>+</sup> HLA-DR Expression and Vita Level in Severe Mycoplasma Pneumoniae Pneumonia

Ling HE; Zehao YU

doi:10.12259/j.issn.2095-610X.S20220528

Volume 43 Issue 5

May 2022

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2022 > 43(5): 103-107

Ling HE, Zehao YU. Correlation between CD3+ HLA-DR Expression and Vita Level in Severe Mycoplasma Pneumoniae Pneumonia[J]. Journal of Kunming Medical University, 2022, 43(5): 103-107. doi: 10.12259/j.issn.2095-610X.S20220528

Citation:

Ling HE, Zehao YU. Correlation between CD3⁺ HLA-DR Expression and Vita Level in Severe Mycoplasma Pneumoniae Pneumonia[J]. Journal of Kunming Medical University, 2022, 43(5): 103-107. doi: 10.12259/j.issn.2095-610X.S20220528

Citation:

PDF( 599 KB)

Correlation between CD3⁺ HLA-DR Expression and Vita Level in Severe Mycoplasma Pneumoniae Pneumonia

doi: 10.12259/j.issn.2095-610X.S20220528

Ling HE,
Zehao YU

Dept. of Pediatrics，The 2nd Affiliated Hospital of Kunming Medical University， Kunming Yunnan 650101，China

Received Date: 2022-01-19
Available Online: 2022-05-12
Publish Date: 2022-05-27

Abstract

Abstract

Objective To investigate the expression of CD3 + HLA-DR in peripheral blood lymphocytes and the level of serum VitA in severe Mycoplasma pneumoniae pneumonia. Methods 24 children who met the diagnostic criteria of severe Mycoplasma pneumoniae pneumonia admitted to the pediatric ward of our hospital from January 2017 to December 2020 were analyzed retrospectively. 47 cases of common Mycoplasma pneumoniae pneumonia hospitalized in the same period were randomly selected as the general group; 23 patients with simple upper respiratory tract infection and short stature were the control group; Fasting venous blood was collected in the morning of the next day after the admission, including non anticoagulant 1ml and anticoagulant 3ml; The antibody titer of Mycoplasma pneumoniae was determined by micro blood passive agglutination method, CD19, CD4, CD8, CD3 + HLA-DR was determined by American Berman flow cytometry and reagent, and serum VA was detected by high performance liquid chromatography; Statistical analysis: one-way ANOVA was used, and LSD-t test was used for pairwise comparison; Pearson analysis was used for correlation analysis; The risk factors were analyzed by logistic analysis. Results 1. There were 24 cases of severe Mycoplasma pneumoniae pneumonia, 14 males and 10 females, aged （4.97 ± 1.79） years; There were 47 cases of Mycoplasma pneumoniae pneumonia, 30 males and 17 females, with an average age of （4.87 ± 1.79） years; In the same period, there were 23 hospitalized children with simple upper respiratory tract infection and short stature, including 12 males and 11 females, with an average age of （4.77 ± 2.11） years. There was no significant difference in age and gender among the three groups （P > 0.05）; 2. Comparison of CD19, CD4, CD8, CD3 + HLA-DR and vita values among the three groups: the expression of CD4, CD3 + HLA-DR and serum Vita levels in lymphocytes of the severe group were significantly lower （34.2 ± 4.54, 11.83 ± 2.57, 0.23 ± 0.10）, CD19 （18.66 ± 2.56） increased significantly, which was significantly higher than that in the general group [38.1 ± 4.04, 17.11 ± 2.71, 0.33 ± 0.14] and the control group （39.35 ± 3.86, 18.78 ± 1.86, 0.40 ± 0.14）（P < 0.05）; Vita was positively correlated with CD4, CD3 + HLA-DR （r = 0.371, 0.688, P < 0.05）. The results of logistic regression analysis showed that the decrease of serum Vita level and CD3 + HLA-DR expression were independent risk factors of SMMP （or = 4.42, 2.38, P < 0.05）. Conclusion lymphocyte activation was involved in the occurrence and development of MPP. Excessive immune response would not only induce excessive immune response, but also lead to autoimmune injury （immune paralysis）. It is speculated that VitA is also involved in regulating lymphocyte activation.
- Mycoplasma pneumoniae pneumonia,
- Severe,
- CD3 + HLA-DR,
- VitA,
- Immune function

FullText(HTML)

References(17)

References

[1]	M Miyata,T Hamaguchi. Prospects for the gliding Mechanism of Mycoplasma mobile[J]. Curr Opin Microbil,2016,29(2):15-20.
[2]	Maya W Haaker,Arie B Vaandrager,J Bernd Helms. Retinoids in health and disease:A role for hepatic stellate cells in affecting retinoid levels[J]. BBA-Molecular and Cell Biology of Lipids,2020,1865(6):158674. doi: 10.1016/j.bbalip.2020.158674
[3]	刘金荣, 赵成松, 赵顺英. 儿童社区获得性肺炎诊疗规范（2019年版）解读[J]. 中国实用儿科杂志, 2020, 35（3）: 185-187.
[4]	国家卫生计生委合理用药专家委员会儿童用药专业组. 中国儿童肺炎支原体感染实验室诊断规范和临床实践专家共识[J]. 中华儿科杂志,2020,58(5):366-370.
[5]	中华预防医学会儿童保健分会. 中国儿童维生素A、维生素D临床应用专家共识[J]. 中国儿童保健杂志,2021,29(1):110-116.
[6]	方柯南,王晶,倪婧雯. 重症肺炎支原体肺炎患儿肺炎支原体DNA复制水平与病情的相关性[J]. 中国当代儿科杂志,2019,21(9):876-880. doi: 10.7499/j.issn.1008-8830.2019.09.006
[7]	余丹阳 . 重视支原体肺炎诊治中面临的困难和挑战[J]. 中华结核和呼吸杂志, 2021, 44（1）: 8-10.
[8]	PM Meyer Sauteur,AM Van Rossum,C Vink. Mycoplasma pneumoniae in children:Carriage,pathogenesis,and antibiotic resistance[J]. Curr Opin Infect Dis,2014,27(3):220-227. doi: 10.1097/QCO.0000000000000063
[9]	蔡辰,胡培培,陆敏,等. 肺泡灌洗液中细胞因子及细胞学水平与重症肺炎支原体肺炎相关性[J]. 中华实用儿科临床杂志,2020,35(18):1421-1424. doi: 10.3760/cma.j.cn101070-20190621-00558
[10]	S Vijayan,T Sidiq,S Yousuf,et al. Class I transactivator,NLRC5:A central player in the MHC class I pathway and cancer immune surveillance[J]. Immunogenetics,2019,71(3):273-282. doi: 10.1007/s00251-019-01106-z
[11]	邵晓丽,刘淑梅,周国忠,等. 单核细胞表面抗原HLA-DR及CD14⁺在肺炎支原体肺炎患儿中的变化[J]. 中华医院感染学杂志,2013,23(7):1500-1502.
[12]	Manzoli T F,Troster E J,Ferranti J F,et al. Prolonged suppression of monocytic human leukocyte antigen-DR expression correlates with mortality in pediatric septic patients in a pediatric tertiary intensive care unit[J]. Crit Care,2016,33(6):84-90.
[13]	Koshelev R V,Vatazin A V,Zulkarnayev A B,et al. The state of the immune system in abdominal sepsis[J]. Ter Arkh,2019,91(2):82-86.
[14]	Mestre-Ferrer A,Scholz E,Humet-Alsius J,et al. PRBAM:A new tool to analyze the MHC class 1 and HLA-DR anchor motifs[J]. Immunology,2019,156(2):187-198. doi: 10.1111/imm.13020
[15]	I H Hiemstra,M R Beijer,H Veninga,et al. The identification and developmental requirements of colonic CD169+ macrophages[J]. Immunology,2014,142(2):269-278.
[16]	Danielle Ter Braake,Naomi Benne,Chun Yin Jerry Lau,et al. Retinoic acid-containing liposomes for the induction of antigen-specific regulatory T cells as a treatment for autoimmune diseases[J]. Pharmaceutics,2021,13(11):1949. doi: 10.3390/pharmaceutics13111949
[17]	杨振宇. 中国居民营养与健康状况监测报告（2010-2013）0-5岁儿童营养与健康状况[M]. 北京: 人民卫生出版社, 2020: 12-14

Relative Articles(20)

Relative Articles

[1]	Zhiye QI, Ying CHEN, Yan LI. Research Progress on Immunotoxicology of Pyrethroid Pesticides Exposures. Journal of Kunming Medical University, 2024, 45(8): 154-160. doi: 10.12259/j.issn.2095-610X.S20240822
[2]	Jihong LIU, Zhiqiang MA, Lin WANG, Xiaoyan WANG, Weiguang TIAN, Baoping LI, Rui DONG, Yujia LV. Correlation Analysis of Immune Function of Tuberculosis Patients Infected with COVID-19. Journal of Kunming Medical University, 2024, 45(4): 157-162. doi: 10.12259/j.issn.2095-610X.S20240423
[3]	Lijuan YIN, Lingyun BAO, Fang WANG, Yuan YUAN. Application Effect of Pulmonary Ultrasound Dynamic Monitoring in Precise Nursing of Severe Neonatal Pneumonia. Journal of Kunming Medical University, 2023, 44(10): 202-206. doi: 10.12259/j.issn.2095-610X.S20231002
[4]	Xiaoli SHANG, Junxue NI, Yeying WANG, Jiao SHAN, Na BAI, Song WANG. Clinical Epidemiological Characteristics of Mycoplasma Pneumoniae and Its Correlation with Climatic Environment. Journal of Kunming Medical University, 2023, 44(6): 97-101. doi: 10.12259/j.issn.2095-610X.S20230618
[5]	Ying ZHANG, Fengxia YANG, Jing GUO, Qingling ZHANG. Effects of Systematic Psychological Intervention Based on S-ABC Framework on Perioperative Psychological Distress and Immune Function in Patients with Primary Liver Cancer. Journal of Kunming Medical University, 2023, 44(11): 170-174. doi: 10.12259/j.issn.2095-610X.S20231126
[6]	Lin WANG, Lingjun SHEN, Haiwen LI, Xiaoyan WANG, Jianpeng GAO, Zhiqiang MA. Analysis of Nutritional Status and Immune Function of Tuberculosis Patients in Yunnan. Journal of Kunming Medical University, 2023, 44(1): 109-115. doi: 10.12259/j.issn.2095-610X.S20230116
[7]	Nihong LU, Honglu LIU, Jiawei XIA, Yangjun CHEN, Linjun SHEN, Yongrui YANG, Jie LI. Clinical Evaluation of Immune Function and SAA, MMP-9 and MMP-14 in Liver Injury Caused by Anti-Tuberculous Drugs. Journal of Kunming Medical University, 2022, 43(4): 50-54. doi: 10.12259/j.issn.2095-610X.S20220410
[8]	Ding Zhen Bo , Duan Jing , Lu Ping , Huang Yong Kun , Zhao Ya Ling , Luo Yan . . Journal of Kunming Medical University, 2020, 41(07): 89-93.
[9]	Sun Yu Xing , Tan Li , Hao Rui , Li Ming , Gan Zheng Yan , Wu Cheng Qing . The Correlation between Exhaled Nitric Oxide and Mycoplasma Pneumoniae Pneumonia in Children. Journal of Kunming Medical University, 2017, 38(09): 79-82.
[10]	Gao Hong . Application Value of Bedside Ultrasound in Diagnosis of Neonatal Severe Pneumonia. Journal of Kunming Medical University,
[11]	Tan Li . Levels of Interleukin-4，Interleukin-6 and Interferon-gamma in Bronchoalveolar Lavaga Fluid of Children with Refractory Mycoplasma Pneumoniae Pneumonia and the Features of Bronchoscopy. Journal of Kunming Medical University,
[12]	Tian Yun Fen . Epidemiological Analysis of Mycoplasma Pneumoniae Infection in Children in Kunming. Journal of Kunming Medical University,
[13]	Hong Xu Hua . Effects of Tuberculosis Drugs Combined with Rifapentine on the Immune Function， Inflammatory Factors， VEGF and MMP-9 of TB Patients. Journal of Kunming Medical University,
[14]	Yang Shao Ling . . Journal of Kunming Medical University,
[15]	Xie Zuo Zhou . . Journal of Kunming Medical University,
[16]	Deng Wei . . Journal of Kunming Medical University,
[17]	Gu Xin . . Journal of Kunming Medical University,
[18]	. Correlation between Pain and Depression and Their Effect on Immune Function in Elderly Cancer Patients. Journal of Kunming Medical University,
[19]	Shen Li Da . . Journal of Kunming Medical University,
[20]	Zhang Yan Li . . Journal of Kunming Medical University,