Analysis of Differential Gene Expression and Function in Peripheral Blood of Patients with Coronary Heart Disease and Acute Myocardial Infarction

Wenqing MIAO; Yu WANG; Xiaoli ZHAO; Nini TIAN; Liying YOU

doi:10.12259/j.issn.2095-610X.S20220614

Volume 43 Issue 6

Jun. 2022

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Wenqing MIAO, Yu WANG, Xiaoli ZHAO, Nini TIAN, Liying YOU. Analysis of Differential Gene Expression and Function in Peripheral Blood of Patients with Coronary Heart Disease and Acute Myocardial Infarction[J]. Journal of Kunming Medical University, 2022, 43(6): 25-34. doi: 10.12259/j.issn.2095-610X.S20220614

Citation:

Wenqing MIAO, Yu WANG, Xiaoli ZHAO, Nini TIAN, Liying YOU. Analysis of Differential Gene Expression and Function in Peripheral Blood of Patients with Coronary Heart Disease and Acute Myocardial Infarction[J]. Journal of Kunming Medical University, 2022, 43(6): 25-34. doi: 10.12259/j.issn.2095-610X.S20220614

Citation:

Wenqing MIAO, Yu WANG, Xiaoli ZHAO, Nini TIAN, Liying YOU. Analysis of Differential Gene Expression and Function in Peripheral Blood of Patients with Coronary Heart Disease and Acute Myocardial Infarction[J]. Journal of Kunming Medical University, 2022, 43(6): 25-34. doi: 10.12259/j.issn.2095-610X.S20220614

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Analysis of Differential Gene Expression and Function in Peripheral Blood of Patients with Coronary Heart Disease and Acute Myocardial Infarction

doi: 10.12259/j.issn.2095-610X.S20220614

Wenqing MIAO^{1, 2},
Yu WANG^{1
,
,},
Xiaoli ZHAO³,
Nini TIAN¹,
Liying YOU²

1.
Dept. of Cardiovascular Medicine，Kunming First People’s Hospital
2.
Dept. of General Medicine，Kunming First People’s Hospital
3.
Clinical Laboratory，Kunming First People’s Hospital，Kunming Yunnan 650011，China

Received Date: 2022-03-06
Available Online: 2022-06-07
Publish Date: 2022-06-30

Abstract

Abstract

Objective To investigate the differential gene expression and function of peripheral blood monocytes in patients with coronary heart disease and acute myocardial infarction. Methods Patients receiving coronary angiography in Kunming First People’s Hospital from September 2020 to September 2021 were collected and total RNA of peripheral blood monocytes was extracted. DNBSEQ platform was used for second-generation high-throughput sequencing. We tested and screened differentially expressed genes, and made KEGG and GO enrichment analysis. Results There were 89 differentially expressed genes, 67 up-regulated genes and 22 down-regulated genes in acute myocardial infarction group compared with normal coronary artery group. Among them, there were 14 differential lncRNAs, 72 differential mRNAs, and 3 differential circRNAs. KEGG pathway enrichment analysis, KEGG disease enrichment analysis and KEGG molecular enrichment analysis were completed. GO cell composition enrichment analysis, GO cell function enrichment analysis and GO biological process molecular enrichment analysis were completed. Conclusions 10 mRNAs, 6 lncRNAs, and 2 circRNAs with significant differences were screened. The enrichment analysis involves infection, transcriptional disorder, PI3K Akt signaling pathway, lipid synthesis, phagocytic vesicle cavity, extracellular matrix, lipopolysaccharide binding, etc., which may be related to the occurrence and development of coronary atherosclerosis and the occurrence of acute thrombotic events.
- Coronary heart disease,
- Acute myocardial infarction,
- Differential gene expression analysis,
- mRNA, lncRNA,circRNA,
- KEGG enrichment analysis,
- GO enrichment analysis

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References(22)

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