Changes of Intestinal Inflammatory Injury Induced by DSS in GC-C<sup>-/-</sup> Mice

Guili LIANG; Canmei LI; Chengfeng XING; Yunlong DONG; Juan WANG; Zi LEI; Yinglei MIAO; Danfeng LAN

doi:10.12259/j.issn.2095-610X.S20220712

Volume 43 Issue 7

Jul. 2022

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Guili LIANG, Canmei LI, Chengfeng XING, Yunlong DONG, Juan WANG, Zi LEI, Yinglei MIAO, Danfeng LAN. Changes of Intestinal Inflammatory Injury Induced by DSS in GC-C-/- Mice[J]. Journal of Kunming Medical University, 2022, 43(7): 1-8. doi: 10.12259/j.issn.2095-610X.S20220712

Citation:

Guili LIANG, Canmei LI, Chengfeng XING, Yunlong DONG, Juan WANG, Zi LEI, Yinglei MIAO, Danfeng LAN. Changes of Intestinal Inflammatory Injury Induced by DSS in GC-C^-/- Mice[J]. Journal of Kunming Medical University, 2022, 43(7): 1-8. doi: 10.12259/j.issn.2095-610X.S20220712

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Changes of Intestinal Inflammatory Injury Induced by DSS in GC-C^-/- Mice

doi: 10.12259/j.issn.2095-610X.S20220712

1.
Dept. of Gastroenterology，The 1st Affilited Hospital of Kunming Medical University，Yunnan Province Clinical Research Center for Digestive Diseases，Kunming Yunnan 650032
2.
Dept. of Oncology，Dali Bai Autonomous Prefecture People’s Hospital，Dali Yunnan 671000
3.
Dept. of Clinical Laboratory
4.
Dept. of Pathology，The 1st Affilited Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2022-04-05
Available Online: 2022-07-01
Publish Date: 2022-07-14

Abstract

Abstract

Objective To investigate the role of guanylate cyclase C （GC-C） signaling pathway in the intestinal inflammatory injury of mice induced by sodium dextran sulfate （DSS）. Methods The colitis models were constructed using DSS in the GC-C gene knockout （GC-C^-/-） mice and wild-type （WT） mice. The mice were divided into the colitis groups which were given drinking 3% DSS solution for one week, and the control groups which were given normal diet. The expressions of GC-C mRNA and protein in colon tissues were detected by the methods of qRT-PCR and western blot. The inflammatory damage of colon tissues was observed by the HE staining and the histopathological score was performed by the light microscope. The levels of inflammatory factors （IL-8 and TNF-α） in the peripheral blood as well as intestinal mucus of mice were determined by ELISA. Results 1. Compared with the WT mice, the disease activity index （DAI） scores of the GC-C^-/- mice induced by DSS increased significantly （P < 0.05）, and the shortening of colon, intestinal thickening, hyperemia and edema were more obvious; 2. The inflammatory damage of colon tissues was more serious and the histopathological score was higher in the GC-C^-/-+DSS group than that of the WT+DSS group significantly （P < 0.05）; 3. The levels of IL-8 and TNF-αin the peripheral blood and intestinal mucus were higher in the GC-C^-/-+DSS group than that of the WT+DSS group significantly （P < 0.05, respectively）. Conclusions The intestinal inflammatory injury is more serious in the GC-C^-/- mice induced by chemical substances. GC-C signaling pathway may be involved in the occurrence and development of ulcerative colitis （UC）.
- Guanylate cyclase C,
- Gene knockout,
- Intestinal inflammation,
- Ulcerative colitis

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